Jesus A. Araujo, Ph.D., M.D.

Laboratory Address:
10833 Le Conte Avenue, CHS 43-254
Los Angeles, CA 90095

Work Address:
10833 Le Conte Avenue, CHS 43-264
Los Angeles, CA 90095

Affiliations
Affiliations
Assistant Professor, Medicine
Environmental Cardiology, UCLA
Member, Cardiology, Adult Cardiac Stress Testing and Echocardiographic Imaging Laboratory
Research Interests
The main research interests of the Araujo Lab are focused on two areas of investigation: 1) Environmental Cardiology: We are dissecting the mechanisms by which exposure to air particulate matter promotes atherosclerosis and ischemic heart disease and studying gene-environment interactions of relevance in the development of cardiovascular disease. In recent years, we have found that air pollutant chemicals such as those present in diesel exhaust particles are able to synergize with oxidized phospholipids generated within oxidized LDL, in the promotion of proatherogenic genes in vascular endothelial cells. We have also determined that inhalation of ambient ultrafine particles lead to systemic prooxidant and proinflammatory effects that result in the development of dysfunctional HDL and enhancement of atherosclerotic lesions. 2) Biology of vascular oxidative stress: We are interested in genes and pathways of relevance in the oxidative stress generated in vascular inflammatory entities such as atherosclerosis and ischemia reperfusion. We have determined that heme oxygenase-1 (HO-1) is an important antioxidant and anti-inflammatory protective gene and together with its transcriptional regulator Nrf2, they may play a central role in orchestrating the antioxidant defense of vascular cells. However, while HO-1 is an anti-atherogenic gene, Nrf2 promotes atherosclerosis instead partly due to the regulation of genes involved in lipid metabolism and cholesterol transport. This underscores t]he complexity of reactive oxygen species (ROS) signaling in vascular cells. We are currently studying how HO-1 expression modulates various inflammatory pathways via the use of genetic and biochemical approaches. There is an important cross-talk between our two areas of investigation as it appears that the generation of ROS in the vasculature and the expression of Nrf2-regulated antioxidant genes represent important elements in the systemic effects of air particulate pollutants.
Biography:

The main research interest of our lab are focused on two areas of investigation:

Environmental Cardiology: we are dissecting the mechanism by which exposure to air particulate matter promotes atherosclerosis and ischemic heart disease and studying gene-environment interactions of relevance in the development of cardiovascular disease. In recent years, we have found that air pollutant chemicals such as those present in diesel exhaust particlare are able to synergize with oxidized phospholipids generated within oxidated LDL, in the promtion of proatherogenic genees in vascular endothelial cells. We have also determined that inhalation of ambient ultrafine particle lead to systemic prooxidant and proinflammatory effects that result in the development of dysfunctional HDL and enhancement of atheroscleorotic leisons.

Biology of vascular oxidative stress: we are interested in genes and pathways of relevence in the oxidative stress generated in vascular inflammatory entities such as atherosclerosis and ischemia reperfusion. We have determined that heme oxygenase-1(HO-1) is an important antioxidant and anti-inflammatory protective gene and together with transcriptional regulator Nrf2, they may play a central role in orchestrating the antioxidant defense of vascular cells. However, while HO-1 is anti-atherogenic gene, NrF2 promotes atherosclerosis instead partly due to the regulation of genes involved in lipid metabolism and cholesterol transport. This underscores the complextiy of reactive oxygen species (ROS) signaling in vascular cells. We are currently studying how HO-1 expression modulates variosu inflammatory pathways via the use of genetic and biochemical approaches.

Publications
Araujo Jesus A Are Ultrafine Particles a Risk Factor for Cardiovascular Diseases?. Revista espanola de cardiologia. 2011; 64(8): 642-645.
Barajas Berenice, Che Nam, Yin Fen, Rowshanrad Amir, Orozco Luz D, Gong Ke Wei, Wang Xuping, Castellani Lawrence W, Reue Karen, Lusis Aldons J, Araujo Jesus A NF-E2-related factor 2 promotes atherosclerosis by effects on plasma lipoproteins and cholesterol transport that overshadow antioxidant protection. Arteriosclerosis, thrombosis, and vascular biology. 2011; 31(1): 58-66.
Romanoski Casey E, Che Nam, Yin Fen, Mai Nguyen, Pouldar Delila, Civelek Mete, Pan Calvin, Lee Sangderk, Vakili Ladan, Yang Wen-Pin, Kayne Paul, Mungrue Imran N, Araujo Jesus A, Berliner Judith A, Lusis Aldons J Network for Activation of Human Endothelial Cells by Oxidized Phospholipids: A Critical Role of Heme Oxygenase 1. Circulation research. 2011; 64(8): .
Araujo Jesus A Particulate air pollution, systemic oxidative stress, inflammation, and atherosclerosis. Air quality, atmosphere, & health. 2010; 4(1): 79-93.
Orozco, L. D. Cokus, S. J. Ghazalpour, A. Ingram-Drake, L. Wang, S. van Nas, A. Che, N. Araujo, J. A. Pellegrini, M. Lusis, A. J. Copy number variation influences gene expression and metabolic traits in mice. Hum Mol Genet. 2009; 18(21): 4118-29.
Araujo, J. A. Nel, A. E. Particulate matter and atherosclerosis: role of particle size, composition and oxidative stress. Part Fibre Toxicol. 2009; 6(number): 24.
Campbell Arezoo, Araujo Jesus A, Li Huihui, Sioutas Constantinos, Kleinman Michael Particulate matter induced enhancement of inflammatory markers in the brains of apolipoprotein E knockout mice. Journal of nanoscience and nanotechnology. 2009; 9(8): 5099-104.
Araujo, J. A. Barajas, B. Kleinman, M. Wang, X. Bennett, B. J. Gong, K. W. Navab, M. Harkema, J. Sioutas, C. Lusis, A. J. Nel, A. E. Ambient particulate pollutants in the ultrafine range promote early atherosclerosis and systemic oxidative stress. Circ Res. 2008; 102(5): 589-96.
Kleinman, M. T. Araujo, J. A. Nel, A. Sioutas, C. Campbell, A. Cong, P. Q. Li, H. Bondy, S. C. Inhaled ultrafine particulate matter affects CNS inflammatory processes and may act via MAP kinase signaling pathways. Toxicol Lett. 2008; 178(2): 127-30.
Gong, K. W. Zhao, W. Li, N. Barajas, B. Kleinman, M. Sioutas, C. Horvath, S. Lusis, A. J. Nel, A. Araujo, J. A. Air-pollutant chemicals and oxidized lipids exhibit genome-wide synergistic effects on endothelial cells. Genome Biol. 2007; 8(7): R149.
Orozco, L. D. Kapturczak, M. H. Barajas, B. Wang, X. Weinstein, M. M. Wong, J. Deshane, J. Bolisetty, S. Shaposhnik, Z. Shih, D. M. Agarwal, A. Lusis, A. J. Araujo, J. A. Heme oxygenase-1 expression in macrophages plays a beneficial role in atherosclerosis. Circ Res. 2007; 100(12): 1703-11.
Gharavi, N. M. Gargalovic, P. S. Chang, I. Araujo, J. A. Clark, M. J. Szeto, W. L. Watson, A. D. Lusis, A. J. Berliner, J. A. High-density lipoprotein modulates oxidized phospholipid signaling in human endothelial cells from proinflammatory to anti-inflammatory. Arterioscler Thromb Vasc Biol. 2007; 27(6): 1346-53.
Ke, B. Shen, X. D. Tsuchihashi, S. Gao, F. Araujo, J. A. Busuttil, R. W. Ritter, T. Kupiec-Weglinski, J. W. Viral interleukin-10 gene transfer prevents liver ischemia-reperfusion injury: Toll-like receptor-4 and heme oxygenase-1 signaling in innate and adaptive immunity. Hum Gene Ther. 2007; 18(4): 355-66.
Tsuchihashi, S. Livhits, M. Zhai, Y. Busuttil, R. W. Araujo, J. A. Kupiec-Weglinski, J. W. Basal rather than induced heme oxygenase-1 levels are crucial in the antioxidant cytoprotection. J Immunol. 2006; 177(7): 4749-57.
Lai, D. W. Saver, J. L. Araujo, J. A. Reidl, M. Tobis, J. Pericarditis associated with nickel hypersensitivity to the Amplatzer occluder device: a case report. Catheter Cardiovasc Interv. 2005; 66(3): 424-6.
Araujo, J. A. Meng, L. Tward, A. D. Hancock, W. W. Zhai, Y. Lee, A. Ishikawa, K. Iyer, S. Buelow, R. Busuttil, R. W. Shih, D. M. Lusis, A. J. Kupiec-Weglinski, J. W. Systemic rather than local heme oxygenase-1 overexpression improves cardiac allograft outcomes in a new transgenic mouse. J Immunol. 2003; 171(3): 1572-80.
Villa-Colinayo, V. Shi, W. Araujo, J. Lusis, A. J. Genetics of atherosclerosis: the search for genes acting at the level of the vessel wall. Curr Atheroscler Rep. 2000; 2(5): 380-9.
Araujo, J. A. Romano, E. L. Brito, B. E. Parthe, V. Romano, M. Bracho, M. Montano, R. F. Cardier, J. Iron overload augments the development of atherosclerotic lesions in rabbits. Arterioscler Thromb Vasc Biol. 1995; 15(8): 1172-80.
Araujo Jesus A, Meng Lingzhong, Tward Aaron D, Hancock Wayne W, Zhai Yuan, Lee Annie, Ishikawa Kazunobu, Iyer Suhasini, Buelow Roland, Busuttil Ronald W, Shih Diana M, Lusis Aldons J, Kupiec-Weglinski Jerzy W Systemic rather than local heme oxygenase-1 overexpression improves cardiac allograft outcomes in a new transgenic mouse. Journal of immunology (Baltimore, Md. : 1950). 2003; 171(3): 1572-80.