Ram Raj Singh, M.D.

Laboratory Address:
1000 Veteran Ave
Los Angeles, CA 90095

Mailing Address:
200 Medical Plaza, Suite 365B
Los Angeles, CA 90095 UCLA Med-Rheum
BOX 951670
35-59 Rehab Center
Los Angeles, CA 90095

Affiliations
Affiliations
Professor, Medicine, Pathology and Laboratory Medicine
Member, Internal Medicine, Immunity, Microbes & Molecular Pathogenesis GPB Home Area, Molecular, Cellular & Integrative Physiology GPB Home Area, Tumor Immunology Program at the Jonsson Comprehensive Cancer Center (JCCC)
Faculty, Cellular and Molecular Pathology PhD Program
Research Interests
Interactions between different immune cells, including dendritic cells, T cells, and B cells, in the pathogenesis of autoimmune diseases, with a current focus on cellular migration, immune tolerance induction, immune modulation, roles of sex chromosomes and sex hormones, epigenetic regulation of immune response genes.
Biography

Ram Raj Singh is a physician-scientist and immunologist who returned to the UCLA School of Medicine as a Professor of Medicine and Pathology in 2004. He serves as a Program Director for an NIH Institutional Training grant in rheumatology and translational immunology. He is also Director of basic and translational research in the division of rheumatology and directs interdisciplinary translational immunology cluster series. Before returning to UCLA, he was Associate Professor (1998-2004) and then Professor (2004) at the University of Cincinnati College of Medicine where he had moved from UCLA after completing his research and fellowship training at UCLA in the Department of Medicine, Division of Rheumatology and Department of Microbiology, Immunology and Molecular Genetics. Dr. Singh received his medical education (MBBS and MD) and training at King George’s Medical College in Lucknow and All-India Institute of Medical sciences in New Delhi. His research on immune pathogenesis of lupus and autoimmune diseases and mechanisms of tolerance has been recognized by over a dozen national and international awards, including Carol Nachman Prize, one of the largest international prize in experimental rheumatology that he received from the President of Germany; Henry Kunkel Young Investigator Award from the American College of Rheumatology; Cutting Edge Lupus Research-Lupus Research Institute award; James R. Klinenberg Science Award from the National Arthritis Foundation; Henry Christian Award (in the Autoimmunity category) from the American Federation for Medical Research; and Science Recognition Award for New Investigators from the Clinical Immunology Society. His research team includes faculty members: Anagha Divekar, PhD, Ramesh Halder, PhD, Suzanne Kafaja, MD, Peter Kim, MD, Jennifer King, MD, Chetachi Okereke, MD, and Janny Zhu, MD, PhD; postdoc fellows Curtis Adams, PhD, Alexandra David, PhD, Rita Okorogu, MD, Julia Pinkhasov, PhD, and Isela Valera, PhD, student Sahar Salehi, volunteer Kelly van der Kar, lab assistant Rachael Philips, and lab manager Dora Acuna, MS. Research in his laboratory is currently funded by NIAID, NIAMS, NICHD, American College of Rheumatology Research and Education Foundation, Arthritis Foundation, and American Heart Association. Dr. Singh’s laboratory is devoted to understanding the cellular and molecular basis of immune-mediated inflammatory diseases such as systemic lupus erythematosus (lupus), scleroderma and rheumatoid arthritis. Currently active projects in his laboratory investigate: a) interactions between dendritic cells and other immune cells within a tissue in causing organ inflammation using skin Langerhans cells, skin gamma delta T cells and lupus dermatitis as model; b) regulation of immune and autoimmune responses by sex chromosomes and hormones; c) CD1d/lipid-reactive T cells in autoimmune diseases, atherosclerosis, vascular thrombosis, and pregnancy loss; d) NKT cell interactions with B cells and other immune cells; e) T cell tolerance and autoreactive T cells; f) mesenchymal stem cells role in immune changes in autoimmune diseases; and g) role of plasmacytoid DCs and T cells in lung and skin inflammation and fibrosis.

Publications

A selected list of publications:

Yang JQ, Kim PJ, Singh RR.   Brief treatment with iNKT cell ligand α-galactosylceramide confers a long-term protection against lupus J Clin Immunol, 2012; 32(1): 106-13.
Khanna D, Saggar R, Mayes MD, Abtin F, Clements PJ, Maranian P, Assassi S, Saggar R, Singh RR, Furst DE.   A one-year, phase I/IIa, open-label pilot trial of imatinib mesylate in the treatment of systemic sclerosis-associated active interstitial lung disease, Arthritis Rheum, 2011; 63(11): 3540-6..
Wen X, Yang JQ, Kim PJ, Singh RR.   Homeostatic regulation of marginal zone B cells by invariant natural killer T cells, PLoS One, 2011; 6(10): e26536.
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Divekar AA, Khanna D, Abtin F, Maranian P, Saggar R, Saggar R, Furst DE, Singh RR.   Treatment with imatinib results in reduced IL-4-producing T cells, but increased CD4(+) T cells in the broncho-alveolar lavage of patients with systemic Clin Immunol, 2011; 141(3): 293-303.
Yang Jun-Qi, Wen Xiangshu, Kim Peter J, Singh Ram Raj   Invariant NKT cells inhibit autoreactive B cells in a contact- and CD1d-dependent manner Journal of immunology (Baltimore, Md. : 1950), 2011; 186(3): 1512-20.
Divekar Anagha A, Dubey Shweta, Gangalum Pallavi R, Singh Ram Raj   Dicer insufficiency and microRNA-155 overexpression in lupus regulatory T cells: an apparent paradox in the setting of an inflammatory milieu Journal of immunology (Baltimore, Md. : 1950), 2011; 186(2): 924-30.
Fulcher Jennifer A, Chang Margaret H, Wang Shuo, Almazan Tim, Hashimi Sara T, Eriksson Anna U, Wen Xiangshu, Pang Mabel, Baum Linda G, Singh Ram Raj, Lee Benhur   Galectin-1 co-clusters CD43/CD45 on dendritic cells and induces cell activation and migration through Syk and protein kinase C signaling The Journal of biological chemistry, 2009; 284(39): 26860-70.
Azhar Mohamad, Yin Moying, Bommireddy Ramireddy, Duffy John J, Yang Junqi, Pawlowski Sharon A, Boivin Gregory P, Engle Sandra J, Sanford L P, Grisham Christina, Singh Ram R, Babcock George F, Doetschman Thomas   Generation of mice with a conditional allele for transforming growth factor beta 1 gene Genesis (New York, N.Y. : 2000), 2009; 47(6): 423-31.
Eriksson Anna U, Singh Ram Raj   Cutting edge: migration of langerhans dendritic cells is impaired in autoimmune dermatitis Journal of immunology (Baltimore, Md. : 1950), 2008; 181(11): 7468-72.
Singh, R.R. Smith-Bouvier, D.L. Divekar, A.A. Sasidhar, M. Arnold, A.P. Voskuhl, R. R.   Gender bias in SLE: Its in your sex chromosomes! Arthritis Rheum, 2008; 58((supplement)): S887.
Eriksson, A.U. Singh, R.R.   Migration of tissue dendritic cells in immune-mediated inflammatory diseases: role of cooperative interaction between epidermal gamma delta T cells and skin dendritic cells (abstract) Experimental Biology, 2008; volume(number): 668.13.
Bommireddy, R. Babcock, G. F. Singh, R. R. Doetschman, T.   TGFbeta1 deficiency does not affect the generation and maintenance of CD4+CD25+FOXP3+ putative Treg cells, but causes their numerical inadequacy and loss of regulatory function Clin Immunol, 2008; 127(2): 206-13.
Smith-Bouvier Deborah L, Divekar Anagha A, Sasidhar Manda, Du Sienmi, Tiwari-Woodruff Seema K, King Jennifer K, Arnold Arthur P, Singh Ram Raj, Voskuhl Rhonda R   A role for sex chromosome complement in the female bias in autoimmune disease The Journal of experimental medicine, 2008; 205(5): 1099-108.
Khurma Vandana, Meyer Cris, Park Grace S, McMahon Maureen, Lin Jan, Singh Ram Raj, Khanna Dinesh   A pilot study of subclinical coronary atherosclerosis in systemic sclerosis: coronary artery calcification in cases and controls Arthritis and rheumatism, 2008; 59(4): 591-7.
Saxena Vijay, Lienesch Douglas W, Zhou Min, Bommireddy Ramireddy, Azhar Mohamad, Doetschman Thomas, Singh Ram Raj   Dual roles of immunoregulatory cytokine TGF-beta in the pathogenesis of autoimmunity-mediated organ damage Journal of immunology (Baltimore, Md. : 1950), 2008; 180(3): 1903-12.
Wong Maida, Ziring David, Korin Yael, Desai Sheetal, Kim Sungjin, Lin Jan, Gjertson David, Braun Jonathan, Reed Elaine, Singh Ram Raj   TNFalpha blockade in human diseases: mechanisms and future directions Clinical immunology (Orlando, Fla.), 2008; 126(2): 121-36.
Lin Jan, Ziring David, Desai Sheetal, Kim Sungjin, Wong Maida, Korin Yael, Braun Jonathan, Reed Elaine, Gjertson David, Singh Ram Raj   TNFalpha blockade in human diseases: an overview of efficacy and safety Clinical immunology (Orlando, Fla.), 2008; 126(1): 13-30.
Hahn, B.H. Singh, R.R.   Animal models of systemic lupus erythematosus Dubois' Lupus Erythematosus, 2007; volume(number): 299-355.
Singh, R. R., Dubey, S.   Autoantigens and defects in immune tolerance in lupus Dubois' Lupus Erythematosus, 2007; volume(number): 370-406.
Keane, M.P. Elashoff, R. Singh, R.R. Chung, J. Belperio, J.A. Goldin, J. Strange, C. Silver, R.M. Tashkin, D.P. Roth, M.D.   Multiple BAL cytokines as predictors of alveolitis In scleroderma lung disease Am J Respir Crit Care Med, 2007; 175(number): A298.
Singh, R.R.   Systemic lupus erythematosus The Year in Rheumatic Disorders, 2007; 6(number): 171-190.
Lin, J Keane, M Roth, M Wang, J Chen, J Zhang, LF Elashoff, R Goldin, J Clements, P Tashkin, DP Furst, DE Singh, RR   TGF-β Superfamily Members and Type 2 Cytokine Transcription Factor Genes in Scleroderma Lung Disease Arthritis Rheum, 2007; volume(number): in press (abstr.).
Yang Jun-Qi, Wen Xiangshu, Liu Hongzhu, Folayan Gbolahan, Dong Xin, Zhou Min, Van Kaer Luc, Singh Ram Raj   Examining the role of CD1d and natural killer T cells in the development of nephritis in a genetically susceptible lupus model Arthritis and rheumatism, 2007; 56(4): 1219-33.
Smith Deborah L, Dong Xin, Du Sienmi, Oh Myungshin, Singh Ram Raj, Voskuhl Rhonda R   A female preponderance for chemically induced lupus in SJL/J mice Clinical immunology (Orlando, Fla.), 2007; 122(1): 101-7.
Bommireddy, R. Pathak, L. J. Martin, J. Ormsby, I. Engle, S. J. Boivin, G. P. Babcock, G. F. Eriksson, A. U. Singh, R. R. Doetschman, T.   Self-antigen recognition by TGFbeta1-deficient T cells causes their activation and systemic inflammation Lab Invest, 2006; 86(10): 1008-19.
Major, A. S. Singh, R. R. Joyce, S. Van Kaer, L.   The role of invariant natural killer T cells in lupus and atherogenesis Immunol Res, 2006; 34(1): 49-66.
Singh Ram Raj   SLE: translating lessons from model systems to human disease Trends in immunology, 2005; 26(11): 572-9.
Hahn BH, Ebling F, Singh RR, Singh RP, Karpouzas G, La Cava A   Cellular and molecular mechanisms of regulation of autoantibody production in lupus, Ann N Y Acad Sci, 2005; 1051: 433-41.
Singh, A. K. Yang, J. Q. Parekh, V. V. Wei, J. Wang, C. R. Joyce, S. Singh, R. R. Van Kaer, L.   The natural killer T cell ligand alpha-galactosylceramide prevents or promotes pristane-induced lupus in mice Eur J Immunol, 2005; 35(4): 1143-54.
Karpouzas George A, La Cava Antonio, Ebling Fanny M, Singh Ram Raj, Hahn Bevra H   Differences between CD8+ T cells in lupus-prone (NZB x NZW) F1 mice and healthy (BALB/c x NZW) F1 mice may influence autoimmunity in the lupus model European journal of immunology, 2004; 34(9): 2489-99.
Adams, D.E. Yang, J.Q. Singh, R.R.   Conditional TGFb receptor II deletion in murine thymocytes results in spontaneous T cell activation and multi-organ inflammation FASEB J, 2004; 18(number): A1136.
Yang Jun-Qi, Chun Taehoon, Liu Hongzhu, Hong Seokmann, Bui Hai, Van Kaer Luc, Wang Chyung-Ru, Singh Ram Raj   CD1d deficiency exacerbates inflammatory dermatitis in MRL-lpr/lpr mice European journal of immunology, 2004; 34(6): 1723-32.
De Albuquerque Deijanira Alves, Saxena Vijay, Adams David E, Boivin Gregory P, Brunner Hermine I, Witte David P, Singh Ram Raj   An ACE inhibitor reduces Th2 cytokines and TGF-beta1 and TGF-beta2 isoforms in murine lupus nephritis Kidney international, 2004; 65(3): 846-59.
Singh Ram Raj   Prevention and control of reciprocal T-B cell diversification: implications for lupus-like autoimmunity Molecular immunology, 2004; 40(14-15): 1137-45.
Yang Jun-Qi, Saxena Vijay, Xu Honglin, Van Kaer Luc, Wang Chyung-Ru, Singh Ram Raj   Repeated alpha-galactosylceramide administration results in expansion of NK T cells and alleviates inflammatory dermatitis in MRL-lpr/lpr mice Journal of immunology (Baltimore, Md. : 1950), 2003; 171(8): 4439-46.
Singh Ram Raj   IL-4 and many roads to lupuslike autoimmunity Clinical immunology (Orlando, Fla.), 2003; 108(2): 73-9.
Yang Jun-Qi, Singh Avneesh K, Wilson Michael T, Satoh Minoru, Stanic Aleksandar K, Park Jang-June, Hong Seokmann, Gadola Stephan D, Mizutani Akiei, Kakumanu Srinivasa R, Reeves Westley H, Cerundolo Vincenzo, Joyce Sebastian, Van Kaer Luc, Singh Ram Raj   Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis Journal of immunology (Baltimore, Md. : 1950), 2003; 171(4): 2142-53.
Saxena, V. Lienesch, D. Singh, R.R.   Mechanisms of chronic kidney damage in lupus: role of Th2 cytokines and TGF-beta FASEB J, 2003; 17(number): C269.
Lienesch, D.W. Adams, D.E. Singh, R.R.   Urinary TGFbeta as a diagnostic marker of kidney damage in chronic lupus nephritis Arthritis Rheum, 2003; 48(number): S593.
Singh Ram Raj, Saxena Vijay, Zang Song, Li Lily, Finkelman Fred D, Witte David P, Jacob Chaim O   Differential contribution of IL-4 and STAT6 vs STAT4 to the development of lupus nephritis Journal of immunology (Baltimore, Md. : 1950), 2003; 170(9): 4818-25.
Bommireddy, R. Saxena, V. Ormsby, I. Yin, M. Boivin, G. P. Babcock, G. F. Singh, R. R. Doetschman, T.   TGF-beta 1 regulates lymphocyte homeostasis by preventing activation and subsequent apoptosis of peripheral lymphocytes J Immunol, 2003; 170(9): 4612-22.
Fan Guo-Chang, Singh Ram Raj   Vaccination with minigenes encoding V(H)-derived major histocompatibility complex class I-binding epitopes activates cytotoxic T cells that ablate autoantibody-producing B cells and inhibit lupus The Journal of experimental medicine, 2002; 196(6): 731-41.
Singh Ram Raj, Ebling Fanny M, Albuquerque D Alves, Saxena Vijay, Kumar Vipin, Giannini Edward H, Marion Tony N, Finkelman Fred D, Hahn Bevra H   Induction of autoantibody production is limited in nonautoimmune mice Journal of immunology (Baltimore, Md. : 1950), 2002; 169(1): 587-94.
Yoshida, H. Satoh, M. Behney, K. M. Lee, C. G. Richards, H. B. Shaheen, V. M. Yang, J. Q. Singh, R. R. Reeves, W. H.   Effect of an exogenous trigger on the pathogenesis of lupus in (NZB x NZW)F1 mice Arthritis Rheum, 2002; 46(8): 2235-44.
Hahn, B. H. Singh, R. R. Wong, W. K. Tsao, B. P. Bulpitt, K. Ebling, F. M.   Treatment with a consensus peptide based on amino acid sequences in autoantibodies prevents T cell activation by autoantigens and delays disease onset in murine lupus Arthritis Rheum, 2001; 44(2): 432-41.
Singh, R. R.   The potential use of peptides and vaccination to treat systemic lupus erythematosus Curr Opin Rheumatol, 2000; 12(5): 399-406.
Singh, R. R. Hahn, B. H. Tsao, B. P. Ebling, F. M.   Evidence for multiple mechanisms of polyclonal T cell activation in murine lupus J Clin Invest, 1998; 102(10): 1841-9.
Singh, R. R. Hahn, B. H.   Reciprocal T-B determinant spreading develops spontaneously in murine lupus: implications for pathogenesis Immunol Rev, 1998; 164(number): 201-8.
Singh, R. R. Hahn, B. H. Sercarz, E. E.   Neonatal peptide exposure can prime T cells and, upon subsequent immunization, induce their immune deviation: implications for antibody vs. T cell-mediated autoimmunity J Exp Med, 1996; 183(4): 1613-21.
Singh, R. R. Ebling, F. M. Sercarz, E. E. Hahn, B. H.   Immune tolerance to autoantibody-derived peptides delays development of autoimmunity in murine lupus J Clin Invest, 1995; 96(6): 2990-6.
Singh, R. R. Kumar, V. Ebling, F. M. Southwood, S. Sette, A. Sercarz, E. E. Hahn, B. H.   T cell determinants from autoantibodies to DNA can upregulate autoimmunity in murine systemic lupus erythematosus J Exp Med, 1995; 181(6): 2017-27.