James U. Bowie

Laboratory Address:
Paul Boyer Hall 659

Work Address:
Paul Boyer Hall 655

Affiliations
Affiliations
Professor, Chemistry and Biochemistry, Biochemistry, Molecular Biology, UCLA-DOE Institute for Genomics & Proteomics, Molecular Biology Institute
Director, Macromolecular Crystallization Core Facility (DOE)
Research Interests
MEMBRANE PROTEIN FOLDING: Protein folding is a fundamental process of life with important implications throughout biology. Elaborate mechanisms exist to regulate and assist folding. Moreover, tens of thousands of mutations have now been associated with diseases and it is thought that most of these mutations affect protein folding and trafficking rather than function. Consequently, there has been an enormous effort over the years to understand how proteins fold. Essentially all of the effort has been directed at soluble proteins, however, and membrane proteins have been largely shunted aside. The lack of effort has not occurred because membrane proteins are unimportant or uninteresting, but because of the great technical challenges they present. It is a challenge that we must overcome if we ever hope to understand membrane biology and disease mechanisms involving a large fraction of the protein universe. We are working to understand folding mechanisms and to develop techniques to study folding and expand the field.

SYNTHETIC BIOCHEMISTRY FOR GREEN PRODUCTION OF CHEMICALS AND BIOFUELS: Considerable effort is currently directed at engineering micro-organisms to produce useful chemicals. The greatest potential environmental benefit of metabolic engineering would be the production of high volume commodity chemicals, such as biofuels. Yet the high yields required for the economic viability of low-value chemicals are particularly hard to achieve in microbes due to the myriad competing biochemical pathways. We are developing an alternative approach, which we call synthetic biochemistry. Synthetic biochemistry throws away the cells and builds biochemical pathways in reaction vessels using complex mixtures of isolated enzymes. As the only pathway in the vessel is the desired transformation, yields can approach 100%. The challenge for synthetic biochemistry is to replace the complex regulatory systems that exist in cells in a simplified form. We are designing and testing various ideas for building highly robust systems that can operate continuously for long periods of time.

Biography

Bowie got hooked on proteins at an early age and never looked back. The obsession began as a high school student when he did a research project on blood clotting enzymes with Kenneth G. Mann at the Mayo Clinic in his hometown of Rochester, MN. The research project won him a trip to the International Science and Engineering fair and a Westinghouse Award, experiences that further stimulated his interest in science. He went on to Carleton College, where he got a B. A. degree in Chemistry with Distinction. He did research in carbohydrate chemistry with Gary R. Gray at the University of Minnesota before entering graduate school at M.I.T. There he returned to proteins in the laboratory of Robert T. Sauer, using experimental and computational methods to probe and predict protein structure. After completing his Ph.D. work, he did postdoctoral work as a Life Sciences Research Foundation fellow with David Eisenberg at UCLA, continuing work on structure prediction and structure determination by x-ray crystallography. He joined the UCLA faculty in 1993 and is now a full professor. His current research focuses on fundamental and practical aspects of membrane protein folding and cell-free methods for the production of commodity chemicals and biofuels. Bowie has served as President of the Protein Society, on NIH study sections, and on the editorial boards of the Journal of Molecular Biology, Protein Science, Biochemistry and Proteins. He has organized many meetings and co-founded a new Gordon Conference on Membrane Protein Folding. Bowie has been recognized as an NSF National Young Investigator, a Pew Scholar, a Leukemia and Lymphoma Society Scholar, a Dreyfus Foundation Mentor, a Fellow of the AAAS, the King’s College Higgs Lecturer, a McCoy Awardee and the Biophysical Society Anatrace Awardee. He is particularly proud to be given the UCLA Postdoctoral Mentoring award based on nominations by his students.

Publications
Min Duyoung, Arbing Mark A, Jefferson Robert E, Bowie James U A simple DNA handle attachment method for single molecule mechanical manipulation experiments. Protein science : a publication of the Protein Society. 2016; 25(8): 1535-44.
Cheng Xi, Kim Jin-Kyoung, Kim Yangmee, Bowie James U, Im Wonpil Molecular dynamics simulation strategies for protein-micelle complexes. Biochimica et biophysica acta. 2016; 1858(7 Pt B): 1566-72.
Opgenorth Paul H, Korman Tyler P, Bowie James U A synthetic biochemistry module for production of bio-based chemicals from glucose. Nature chemical biology. 2016; 12(6): 393-5.
Sherkhanov Saken, Korman Tyler P, Clarke Steven G, Bowie James U Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme. Scientific reports. 2016; 6(6): 24239.
Spielmann Malte, Kakar Naseebullah, Tayebi Naeimeh, Leettola Catherine, Nürnberg Gudrun, Sowada Nadine, Lupiáñez Darío G, Harabula Izabela, Flöttmann Ricarda, Horn Denise, Chan Wing Lee, Wittler Lars, Yilmaz Rüstem, Altmüller Janine, Thiele Holger, van Bokhoven Hans, Schwartz Charles E, Nürnberg Peter, Bowie James U, Ahmad Jamil, Kubisch Christian, Mundlos Stefan, Borck Guntram Exome sequencing and CRISPR/Cas genome editing identify mutations of ZAK as a cause of limb defects in humans and mice. Genome research. 2016; 26(2): 183-91.
Woodall Nicholas B, Yin Ying, Bowie James U Dual-topology insertion of a dual-topology membrane protein. Nature communications. 2015; 6(12): 8099.
Min Duyoung, Jefferson Robert E, Bowie James U, Yoon Tae-Young Mapping the energy landscape for second-stage folding of a single membrane protein. Nature chemical biology. 2015; 11(12): 981-7.
Nam Hyun-Jun, Kim Inhae, Bowie James U, Kim Sanguk Metazoans evolved by taking domains from soluble proteins to expand intercellular communication network. Scientific reports. 2015; 5(12): 9576.
Vinokur Jeffrey M, Korman Tyler P, Sawaya Michael R, Collazo Michael, Cascio Duillio, Bowie James U Structural analysis of mevalonate-3-kinase provides insight into the mechanisms of isoprenoid pathway decarboxylases. Protein science : a publication of the Protein Society. 2015; 24(2): 212-20.
Schlebach Jonathan P, Woodall Nicholas B, Bowie James U, Park Chiwook Bacteriorhodopsin folds through a poorly organized transition state. Journal of the American Chemical Society. 2014; 136(47): 16574-81.
Leettola Catherine N, Knight Mary Jane, Cascio Duilio, Hoffman Sigrid, Bowie James U Characterization of the SAM domain of the PKD-related protein ANKS6 and its interaction with ANKS3. BMC structural biology. 2014; 14(47): 17.
Sherkhanov Saken, Korman Tyler P, Bowie James U Improving the tolerance of Escherichia coli to medium-chain fatty acid production. Metabolic engineering. 2014; 25(47): 1-7.
Opgenorth Paul H, Korman Tyler P, Bowie James U A synthetic biochemistry molecular purge valve module that maintains redox balance. Nature communications. 2014; 5(25): 4113.
Vinokur Jeffrey M, Korman Tyler P, Cao Zheng, Bowie James U Evidence of a novel mevalonate pathway in archaea. Biochemistry. 2014; 53(25): 4161-8.
Korman Tyler P, Sahachartsiri Bobby, Li Dan, Vinokur Jeffrey M, Eisenberg David, Bowie James U A synthetic biochemistry system for the in vitro production of isoprene from glycolysis intermediates. Protein science : a publication of the Protein Society. 2014; 23(5): 576-85.
Cao Zheng, Bowie James U An energetic scale for equilibrium H/D fractionation factors illuminates hydrogen bond free energies in proteins. Protein science : a publication of the Protein Society. 2014; 23(5): 566-75.
Chang Yu-Chu, Bowie James U Measuring membrane protein stability under native conditions. Proceedings of the National Academy of Sciences of the United States of America. 2014; 111(1): 219-24.
Hong Heedeok, Chang Yu-Chu, Bowie James U Measuring transmembrane helix interaction strengths in lipid bilayers using steric trapping. Methods in molecular biology (Clifton, N.J.). 2013; 1063(40): 37-56.
Jefferson Robert E, Blois Tracy M, Bowie James U Membrane proteins can have high kinetic stability. Journal of the American Chemical Society. 2013; 135(40): 15183-90.
Nam Hyun-Jun, Han Seong Kyu, Bowie James U, Kim Sanguk Rampant exchange of the structure and function of extramembrane domains between membrane and water soluble proteins. PLoS computational biology. 2013; 9(3): e1002997.
Korman Tyler P, Sahachartsiri Bobby, Charbonneau David M, Huang Grace L, Beauregard Marc, Bowie James U Dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution. Biotechnology for biofuels. 2013; 6(1): 70.
Bowie James U Structural biology. Membrane protein twists and turns. Science (New York, N.Y.). 2013; 339(6118): 398-9.
Korman Tyler P, Bowie James U Crystal structure of Proteus mirabilis lipase, a novel lipase from the Proteus/psychrophilic subfamily of lipase family I.1. PloS one. 2012; 7(12): e52890.
Meruelo Alejandro D, Han Seong Kyu, Kim Sanguk, Bowie James U Structural differences between thermophilic and mesophilic membrane proteins. Protein science : a publication of the Protein Society. 2012; 21(11): 1746-53.
Kim Jinho, Kim Inhae, Han Seong Kyu, Bowie James U, Kim Sanguk Network rewiring is an important mechanism of gene essentiality change. Scientific reports. 2012; 2(3): 900.
Cao Zheng, Bowie James U Shifting hydrogen bonds may produce flexible transmembrane helices. Proceedings of the National Academy of Sciences of the United States of America. 2012; 109(21): 8121-6.
Cao Zheng, Schlebach Jonathan P, Park Chiwook, Bowie James U Thermodynamic stability of bacteriorhodopsin mutants measured relative to the bacterioopsin unfolded state. Biochimica et biophysica acta. 2012; 1818(4): 1049-54.
Schlebach Jonathan P, Cao Zheng, Bowie James U, Park Chiwook Revisiting the folding kinetics of bacteriorhodopsin. Protein science : a publication of the Protein Society. 2012; 21(1): 97-106.
Ujwal Rachna, Bowie James U Crystallizing membrane proteins using lipidic bicelles. Methods (San Diego, Calif.). 2011; 55(4): 337-41.
Stafford Ryan L, Hinde Elizabeth, Knight Mary Jane, Pennella Mario A, Ear Jason, Digman Michelle A, Gratton Enrico, Bowie James U Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure (London, England : 1993). 2011; 19(12): 1826-36.
Knight Mary Jane, Leettola Catherine, Gingery Mari, Li Hao, Bowie James U A human sterile alpha motif domain polymerizome. Protein science : a publication of the Protein Society. 2011; 20(10): 1697-706.
Stafford Ryan L, Ear Jason, Knight Mary Jane, Bowie James U The molecular basis of the Caskin1 and Mint1 interaction with CASK. Journal of molecular biology. 2011; 412(1): 3-13.
Hong Heedeok, Bowie James U Dramatic destabilization of transmembrane helix interactions by features of natural membrane environments. Journal of the American Chemical Society. 2011; 133(29): 11389-98.
Meruelo Alejandro D, Samish Ilan, Bowie James U TMKink: a method to predict transmembrane helix kinks. Protein science : a publication of the Protein Society. 2011; 20(7): 1256-64.
Stafford Ryan L, Tang Ming-Yun, Sawaya Michael R, Phillips Martin L, Bowie James U Crystal structure of the central coiled-coil domain from human liprin-β2. Biochemistry. 2011; 50(18): 3807-15.
Schlebach Jonathan P, Kim Moon-Soo, Joh Nathan H, Bowie James U, Park Chiwook Probing membrane protein unfolding with pulse proteolysis. Journal of molecular biology. 2011; 406(4): 545-51.
Bowie James U Membrane protein folding: how important are hydrogen bonds?. Current opinion in structural biology. 2011; 21(1): 42-9.
Hong Heedeok, Blois Tracy M, Cao Zheng, Bowie James U Method to measure strong protein-protein interactions in lipid bilayers using a steric trap. Proceedings of the National Academy of Sciences of the United States of America. 2010; 107(46): 19802-7.
Knight Mary Jane, Joubert Marisa K, Plotkowski Megan L, Kropat Janette, Gingery Mari, Sakane Fumio, Merchant Sabeeha S, Bowie James U Zinc Binding Drives Sheet Formation by the SAM Domain of Diacylglycerol Kinase δ. Biochemistry. 2010; 107(46): .
Ryan Christopher M, Souda Puneet, Bassilian Sara, Ujwal Rachna, Zhang Jun, Abramson Jeff, Ping Peipei, Durazo Armando, Bowie James U, Hasan S Saif, Baniulis Danas, Cramer William A, Faull Kym F, Whitelegge Julian P Post-translational modifications of integral membrane proteins resolved by top-down Fourier transform mass spectrometry with collisionally activated dissociation. Molecular & cellular proteomics : MCP. 2010; 9(5): 791-803.
Di Pietro Santiago M, Cascio Duilio, Feliciano Daniel, Bowie James U, Payne Gregory S Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain. The EMBO journal. 2010; 29(6): 1033-44.
Blois Tracy M, Hong Heedeok, Kim Tae H, Bowie James U Protein unfolding with a steric trap. Journal of the American Chemical Society. 2009; 131(39): 13914-5.
Oberai Amit, Joh Nathan H, Pettit Frank K, Bowie James U Structural imperatives impose diverse evolutionary constraints on helical membrane proteins. Proceedings of the National Academy of Sciences of the United States of America. 2009; 106(42): 17747-50.
Joh Nathan H, Oberai Amit, Yang Duan, Whitelegge Julian P, Bowie James U Similar energetic contributions of packing in the core of membrane and water-soluble proteins. Journal of the American Chemical Society. 2009; 131(31): 10846-7.
Blois Tracy M, Bowie James U G-protein-coupled receptor structures were not built in a day. Protein science : a publication of the Protein Society. 2009; 18(7): 1335-42.
Massey-Gendel Elizabeth, Zhao Anni, Boulting Gabriella, Kim Hye-Yeon, Balamotis Michael A, Seligman Len M, Nakamoto Robert K, Bowie James U Genetic selection system for improving recombinant membrane protein expression in E. coli. Protein science : a publication of the Protein Society. 2009; 18(2): 372-83.
Hong Heedeok, Joh Nathan H, Bowie James U, Tamm Lukas K Methods for measuring the thermodynamic stability of membrane proteins. Methods in enzymology. 2009; 455(2): 213-36.
Meruelo Alejandro D, Bowie James U Identifying polymer-forming SAM domains. Proteins. 2009; 74(1): 1-5.
Joh Nathan Hyunjoong, Min Andrew, Faham Salem, Whitelegge Julian P, Yang Duan, Woods Virgil L, Bowie James U Modest stabilization by most hydrogen-bonded side-chain interactions in membrane proteins. Nature. 2008; 453(7199): 1266-70.
Harada Bryan T, Knight Mary Jane, Imai Shin-Ichi, Qiao Feng, Ramachander Ranjini, Sawaya Michael R, Gingery Mari, Sakane Fumio, Bowie James U Regulation of enzyme localization by polymerization: polymer formation by the SAM domain of diacylglycerol kinase delta1. Structure. 2008; 16(3): 380-7.
Gorman Paul M, Kim Sanguk, Guo Meng, Melnyk Roman A, McLaurin Joanne, Fraser Paul E, Bowie James U, Chakrabartty Avijit Dimerization of the transmembrane domain of amyloid precursor proteins and familial Alzheimer's disease mutants. J. Mol. Biol. 2008; 9(4): 17.
Sapra K Tanuj, Balasubramanian G Prakash, Labudde Dirk, Bowie James U, Muller Daniel J Point mutations in membrane proteins reshape energy landscape and populate different unfolding pathways. J. Mol. Biol. 2008; 376(4): 1076-90.
Nauli Sehat, Farr Saman, Lee Yueh-Jung, Kim Hye-Yeon, Faham Salem, Bowie James U Polymer-driven crystallization. Protein Sci. 2007; 16(11): 2542-51.
Plotkowski Megan L, Kim Sanguk, Phillips Martin L, Partridge Anthony W, Deber Charles M, Bowie James U Transmembrane domain of myelin protein zero can form dimers: possible implications for myelin construction. Biochemistry. 2007; 46(43): 12164-73.
Pettit Frank K, Bare Emiko, Tsai Albert, Bowie James U HotPatch: a statistical approach to finding biologically relevant features on protein surfaces. J. Mol. Biol. 2007; 369(3): 863-79.
Barwe Sonali P, Kim Sanguk, Rajasekaran Sigrid A, Bowie James U, Rajasekaran Ayyappan K Janus model of the Na,K-ATPase beta-subunit transmembrane domain: distinct faces mediate alpha/beta assembly and beta-beta homo-oligomerization. J. Mol. Biol. 2007; 365(3): 706-14.
Oberai Amit, Ihm Yungok, Kim Sanguk, Bowie James U A limited universe of membrane protein families and folds. Protein Sci. 2006; 15(7): 1723-34.
Gundelfinger Eckart D, Boeckers Tobias M, Baron Marisa K, Bowie James U A role for zinc in postsynaptic density asSAMbly and plasticity?. Trends Biochem. Sci. 2006; 31(7): 366-73.
Bowie James U Flip-flopping membrane proteins. Nat. Struct. Mol. Biol. 2006; 13(2): 94-6.
Baron Marisa K, Boeckers Tobias M, Vaida Bianca, Faham Salem, Gingery Mari, Sawaya Michael R, Salyer Danielle, Gundelfinger Eckart D, Bowie James U An architectural framework that may lie at the core of the postsynaptic density. Science. 2006; 311(5760): 531-5.
Qiao Feng, Harada Bryan, Song Haiyun, Whitelegge Julian, Courey Albert J, Bowie James U Mae inhibits Pointed-P2 transcriptional activity by blocking its MAPK docking site. EMBO J. 2006; 25(1): 70-9.
Bowie James U Solving the membrane protein folding problem. Nature. 2005; 438(7068): 581-9.
Kim Sanguk, Jeon Tae-Joon, Oberai Amit, Yang Duan, Schmidt Jacob J, Bowie James U Transmembrane glycine zippers: physiological and pathological roles in membrane proteins. Proc. Natl. Acad. Sci. U.S.A. 2005; 102(40): 14278-83.
Lorch Mark, Faham Salem, Kaiser Christoph, Weber Ingrid, Mason A James, Bowie James U, Glaubitz Clemens How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Chembiochem : a European journal of chemical biology. 2005; 6(9): 1693-700.
Song Haiyun, Nie Minghua, Qiao Feng, Bowie James U, Courey Albert J Antagonistic regulation of Yan nuclear export by Mae and Crm1 may increase the stringency of the Ras response. Genes & development. 2005; 19(15): 1767-72.
Kim Chongwoo A, Sawaya Michael R, Cascio Duilio, Kim Woojae, Bowie James U Structural organization of a Sex-comb-on-midleg/polyhomeotic copolymer. The Journal of biological chemistry. 2005; 280(30): 27769-75.
Qiao Feng, Bowie James U The many faces of SAM. Science's STKE : signal transduction knowledge environment. 2005; 2005(286): re7.
Faham Salem, Boulting Gabriella L, Massey Elizabeth A, Yohannan Sarah, Yang Dawn, Bowie James U Crystallization of bacteriorhodopsin from bicelle formulations at room temperature. Protein science : a publication of the Protein Society. 2005; 14(3): 836-40.
Edwards Michelle D, Li Yuezhou, Kim Sanguk, Miller Samantha, Bartlett Wendy, Black Susan, Dennison Sally, Iscla Irene, Blount Paul, Bowie James U, Booth Ian R Pivotal role of the glycine-rich TM3 helix in gating the MscS mechanosensitive channel. Nature structural & molecular biology. 2005; 12(2): 113-9.
Partridge Anthony W, Liu Shouchun, Kim Sanguk, Bowie James U, Ginsberg Mark H Transmembrane domain helix packing stabilizes integrin alphaIIbbeta3 in the low affinity state. The Journal of biological chemistry. 2005; 280(8): 7294-300.
Bowie James U Cell biology: border crossing. Nature. 2005; 433(7024): 367-9.
Chamberlain Aaron K, Bowie James U Analysis of side-chain rotamers in transmembrane proteins. Biophysical journal. 2004; 87(5): 3460-9.
Ramachander Ranjini, Bowie James U SAM domains can utilize similar surfaces for the formation of polymers and closed oligomers. Journal of molecular biology. 2004; 342(5): 1353-8.
Kim Sanguk, Chamberlain Aaron K, Bowie James U A model of the closed form of the nicotinic acetylcholine receptor m2 channel pore. Biophysical journal. 2004; 87(2): 792-9.
Chamberlain Aaron K, Bowie James U Asymmetric amino acid compositions of transmembrane beta-strands. Protein science : a publication of the Protein Society. 2004; 13(8): 2270-4.
Qiao Feng, Song Haiyun, Kim Chongwoo A, Sawaya Michael R, Hunter Jacob B, Gingery Mari, Rebay Ilaria, Courey Albert J, Bowie James U Derepression by depolymerization; structural insights into the regulation of Yan by Mae. Cell. 2004; 118(2): 163-73.
Yohannan Sarah, Yang Duan, Faham Salem, Boulting Gabriella, Whitelegge Julian, Bowie James U Proline substitutions are not easily accommodated in a membrane protein. Journal of molecular biology. 2004; 341(1): 1-6.
Chamberlain Aaron K, Lee Yohan, Kim Sanguk, Bowie James U Snorkeling preferences foster an amino acid composition bias in transmembrane helices. Journal of molecular biology. 2004; 339(2): 471-9.
Kim Sanguk, Chamberlain Aaron K, Bowie James U Membrane channel structure of Helicobacter pylori vacuolating toxin: role of multiple GXXXG motifs in cylindrical channels. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101(16): 5988-91.
Yamaguchi Satoru, Tuzi Satoru, Bowie James U, Saitô Hazime Secondary structure and backbone dynamics of Escherichia coli diacylglycerol kinase, as revealed by site-directed solid-state 13C NMR. Biochimica et biophysica acta. 2004; 1698(1): 97-105.
Melnyk Roman A, Kim Sanguk, Curran A Rachael, Engelman Donald M, Bowie James U, Deber Charles M The affinity of GXXXG motifs in transmembrane helix-helix interactions is modulated by long-range communication. The Journal of biological chemistry. 2004; 279(16): 16591-7.
Yohannan Sarah, Faham Salem, Yang Duan, Grosfeld David, Chamberlain Aaron K, Bowie James U A C alpha-H...O hydrogen bond in a membrane protein is not stabilizing. Journal of the American Chemical Society. 2004; 126(8): 2284-5.
Bowie James U Membrane proteins: a new method enters the fold. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101(12): 3995-6.
Faham Salem, Yang Duan, Bare Emiko, Yohannan Sarah, Whitelegge Julian P, Bowie James U Side-chain contributions to membrane protein structure and stability. Journal of molecular biology. 2004; 335(1): 297-305.
Salwinski Lukasz, Miller Christopher S, Smith Adam J, Pettit Frank K, Bowie James U, Eisenberg David The Database of Interacting Proteins: 2004 update. Nucleic acids research. 2004; 32(Database issue): D449-51.
Yohannan Sarah, Faham Salem, Yang Duan, Whitelegge Julian P, Bowie James U The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptors. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101(4): 959-63.
Rajasekaran Sigrid A, Anilkumar Gopalakrishnapillai, Oshima Eri, Bowie James U, Liu He, Heston Warren, Bander Neil H, Rajasekaran Ayyappan K A novel cytoplasmic tail MXXXL motif mediates the internalization of prostate-specific membrane antigen. Molecular biology of the cell. 2003; 14(12): 4835-45.
Kim Chongwoo A, Bowie James U SAM domains: uniform structure, diversity of function. Trends in biochemical sciences. 2003; 28(12): 625-8.
Kim Sanguk, Chamberlain Aaron K, Bowie James U A simple method for modeling transmembrane helix oligomers. Journal of molecular biology. 2003; 329(4): 831-40.
Partridge Anthony W, Melnyk Roman A, Yang Dawn, Bowie James U, Deber Charles M A transmembrane segment mimic derived from Escherichia coli diacylglycerol kinase inhibits protein activity. The Journal of biological chemistry. 2003; 278(24): 22056-60.
Chamberlain Aaron K, Faham Salem, Yohannan Sarah, Bowie James U Construction of helix-bundle membrane proteins. Advances in protein chemistry. 2003; 63(4): 19-46.
Ramachander Ranjini, Kim Chongwoo A, Phillips Martin L, Mackereth Cameron D, Thanos Christopher D, McIntosh Lawrence P, Bowie James U Oligomerization-dependent association of the SAM domains from Schizosaccharomyces pombe Byr2 and Ste4. The Journal of biological chemistry. 2002; 277(42): 39585-93.
Chamberlain Aaron K, Bowie James U Evaluation of C-H cdots, three dots, centered O hydrogen bonds in native and misfolded proteins. Journal of molecular biology. 2002; 322(3): 497-503.
Tran Hoang H, Kim Chongwoo A, Faham Salem, Siddall Marie-Claire, Bowie James U Native interface of the SAM domain polymer of TEL. BMC structural biology. 2002; 2(3): 5.
Kim Chongwoo A, Gingery Mari, Pilpa Rosemarie M, Bowie James U The SAM domain of polyhomeotic forms a helical polymer. Nature structural biology. 2002; 9(6): 453-7.
Faham Salem, Bowie James U Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure. Journal of molecular biology. 2002; 316(1): 1-6.