Ann M. Hirsch, Ph.D.

Laboratory Address:
618 Charles Young Drive South
Los Angeles, CA 90095

Mailing Address:
621 Charles Young Drive S.
MCDB Department
UCLA
Los Angeles, CA 90095

Affiliations
Affiliations
Member, Molecular, Cell, and Developmental Biology, Genetics & Genomics GPB Home Area, Immunity, Microbes & Molecular Pathogenesis GPB Home Area
Research Interests
According to D.H. Jansen, the plant root can be described as the plant gut because like the gut, the root-bacteria not only are associated with the outside of the organ and provide nutrients, but they also influence developmental processes in the root. One of the most investigated of these is the development of the nitrogen fixing nodule. However, many other bacteria are found in the plant's surroundings, the rhizosphere. We want to know how many of these are absolutely essential for plant survival in the environment and can we understand how to produce microbial transplantation experiments to restore degrade soils for food and biofuel production.
Biography

Dr. Ann M. Hirsch joined UCLA in 1988 and has been a full professor in the Department of Molecular, Cell and Developmental Biology since 1992. Dr. Hirsch earned her B.S. degree in Biology with a minor in Chemistry at Marquette University in Wisconsin, and earned her Ph.D. in Botany at the University of California-Berkeley. She did her postdoctoral research at Harvard University in Cambridge, MA, where she began her research on plant-microbe interactions. Dr. Hirsch has been actively involved in both graduate and undergraduate education and also in international education and research collaborations.

Publications
Fujishige, N.A., Lum, M.R., De Hoff, P.L., Whitelegge, J.P., Faull, K.F., and Hirsch, A.M. Rhizobium common nod genes are required for biofilm formation. Mol. Micro. 2008; 67: 504-515.
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Woo, H.-H., Jeong, B.R., Hirsch, A.M., and Hawes, M.C. Altered expression of closely related UDP-glycosyltransferases from pea and Arabidopsis results in altered root development and function. Physiol. Plant. 2007; 130: 250-260.
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Lee, A. and Hirsch, A.M. Signals and responses: choreographing the complex interaction between legumes and alpha- and beta-rhizobia. Plant Signal. Behavior 2006; 1(4): 290-304.
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Rinaudi, L., Fujishige, N.A., Hirsch, A.M., Banchio, E., Zorreguieta, and Giordano, W. Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation. Res. Microbiol. 2006; 157: 867-875.
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Fujishige, N.A., Kapadia, N.N., De Hoff, P.L., and Hirsch, A.M. Investigations of Rhizobium biofilm formation. FEMS Microbiol. Ecol. 2006; 56: 195-205.
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Fujishige, N.A., Kapadia, N.N. and Hirsch, A.M. A feeling for the microorganism: structure on a small scale. Biofilms on plant roots. Bot. J. Linn. Soc 2006; 50:: 79-88.
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Valdes, M., Perez, N.-O., Estrada-de los Santos, P., Caballero-Mellado, J., Pena-Cabriales J.J, Normand, P., and Hirsch, A.M. Non-Frankia actinomycetes isolated from surface-sterilized roots of Casuarina equisetifolia fix nitrogen . Appl. Environ. Microbiol . 2005; 71: 460-466.
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Giordano, W. and Hirsch, A.M. The expression of MaEXP1, a Melilotus alba expansin gene is up-regulated during the sweetclover-Sinorhizobium meliloti interaction. Mol. Plant-Microbe Inter 2004; 17: 613-622.
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Karlowski, W.M., Hirsch, A.M. The overexpression of an alfalfa RING-H2 gene induces pleiotropic effects on plant growth and development. Plant Mol. Biol. 2003; 52: 121-133.
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Lum, M., Li, Y., LaRue, T.A., Schwartz, R.D., Kapulnik, Y., Hirsch, A.M. Investigation of four non-nodulating mutants of white sweetclover (Melilotus alba annua Desr.) mutants and their response to arbuscular-mycorrhizal fungi. Int. Comp. Biol 2002; 42: 113-121.
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Leroy, A., Potter, E., Woo, H.-H., Heber, D., Hirsch, A.M PCR-based identification of degraded DNA and monitoring of flavonoids isolated from alfalfa and red clover commercial preparations used as botanicals in dietary supplements. J. Agr. Food Chem. 2002; 50: 5063-5069.
van Rhijn, P., Fujishige, N.A., Lim, P.-O., and Hirsch, A.M. Sugar-binding activity of pea (Pisum sativum) lectin is essential for heterologous infection of transgenic alfalfa (Medicago sativa L.) plants by Rhizobium leguminosarum biovar viciae. Plant Physiol. 2001; 125: 133-144.
Brill LM, Evans CJ, Hirsch AM Expression of MsLEC1- and MsLEC2-antisense genes in alfalfa plant lines causes severe embryogenic, developmental and reproductive abnormalities. Plant J. 2001; 25(4): 453-461.
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van Rhijn P, Goldberg RB, Hirsch, AM Lotus corniculatus nodulation specificity is changed by the presence of a soybean lectin gene . Plant Cell. 1998; 10(8): 1233-1250.
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van Rhijn, P., Fang, Y., Galili, S., Shaul, O., Atzmon, N., Winiger, S., Eshead, Y., Lum, M., Li, Y., To, V., Fujishige, N., Kapulnik, Y., and Hirsch, A.M. Expression of early nodulin genes in alfalfa mycorrhizae indicates that signal transduction pathways used in forming arbuscular mycorrhizae and Rhizobium-induced nodules may be conserved. Proc. Natl. Acad. Sci. (USA) . 1997; 94: 5467-5472.
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Suzuki, A., Suriyagoda, L, Shigeyama, T., Tominaga, A., Sasaki, M., Hiratsuka, Y., Yoshinaga A., Arima, S., Agarie, S., Sakai, T., Inada, S., Jikumaru, Y, Kamiya, Y., Uchiumi, T., Abe, M., Hashiguchi, M., Akashi, T., Sato, S., Kaneko, T., Tabata, S., and Hirsch, A.M. Lotus japonicus nodulation is photomorphogenetically controlled by sensing the R/FR ratio through JA signaling. . Proc. Natl. Acad. Sci. USA. . 2011; 108: 16837-16842.
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Gyaneshwar, P., Hirsch, A.M., Moulin L, Chen, W.M., Elliott, G.N., Bontemps, C., Estrada-de los Santos, P., Gross, E., Bueno dos Reis Junior, F., Sprent, J.I., Young, J.P.W., and James, E.K. Legume nodulating β-proteobacteria: diversity, host range and future prospects. Molecular Plant-Microbe Interactions 2011; 24: 1276-1288.
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Robledo, M., Rivera, I.P., Jiménez-Zurdo, J.I., Rivas, R., Dazzo, F.B., Velázquez, E., Martínez-Molina, E., Hirsch, A.M., Mateos, P.F. Role of Rhizobium endoglucanase CelC2 in cellulose biosynthesis and biofilm formation on plant roots and abiotic surfaces. . Microbial Cell Factories 2012; 11: 125.
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Angus, A.A., Lee, A.S., Lum, M.R., Shehayeb, M., Hessabi, R., Fujishige, N.A., Yerrapragada S., Kano, S., Song, N., Yang, P., Estrada-de Los Santos, P., de Faria, S.M., Dakora, F.D., Weinstock, G., and Hirsch, A.M. Nodulation and effective nitrogen fixation of Macroptilium atropurpureum (siratro) by Burkholderia tuberum, a beta-proteobacterium, are influenced by environmental factors. . Plant and Soil 2013; 362(543-562): .
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Estrada-de los Santos, P., Vinuesa, P., Martínez-Aguilar, L., Hirsch, A.M., and Caballero-Mellado, J. Phylogenetic analysis of Burkholderia species by Multilocus Sequence Analysis. Current Biology 2013; 67: 51-60.
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Kaplan, D., Maymon M., Agapakis, C.M. Lee, A., Wang, A., Prigge, B.A., Volkogon, M. and Hirsch, A.M. A survey of the microbial community in the rhizosphere of the dominant plant of the Negev Desert, Zygophyllum dumosum Boiss., using cultivation-dependent and –independent methods. American Journal of Botany. 2013; 100: .
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Schwartz, A.R., Ortiz, I., Maymon, M., Fujishige, N.A. Hanamoto, K., Diener, A., Sanders, E.R., DeMason, D.A., and Hirsch, A.M. Bacillus simplex alters legume root architecture and nodule morphology when co-inoculated with Rhizobium. agronomy 2013; 3: 595-620.
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Martínez-Aguilar, L. Salazar-Salazar, C., Caballero-Mellado, J., Hirsch, A.M., Vásquez-Murrieta, M.S., and Estrada-de los Santos, P. Burkholderia caballeronis sp. nov., a nitrogen fixing species isolated from tomato (Lycopersion esculentum) with the ability to nodulate Phaseolus vulgaris effectively. A. Van Leeuwenhoek 2013; 6: 1063-1071.
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Angus, A.A., Agapakis, C.M., Fong, S., Yerrapragada, S., Estrada-de los Santos, P., Yang, P., Song, N., Kano, S., Caballero-Mellado, J., de Faria, S.M., Dakora, F.D., Weinstock, G., and Hirsch, A.M. Plant-associated symbiotic Burkholderia species lack hallmark strategies required in mammalian pathogenesis. . PLoS ONE . 2014; 9(1): e83779.
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Sanders, E.R., and Hirsch, A.M. Immersing undergraduate students into research on the metagenomics of the plant rhizosphere: a pedagogical strategy to engage civic-mindedness and retain undergraduates in STEM. . Front. Plant Sci. . 2014; 5: 157.
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Moraes, D.F.C., Still D., Lum, M.R., Hirsch, A.M. DNA-based authentication of botanicals and plant-derived dietary supplements: where have we been and where are we going? . Planta Medica. 2015; 81: 687-695.
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Amaya-Gómez, C.V., Hirsch, A.M., and Soto, M.J. Biofilm formation assessment in Sinorhizobium meliloti reveals interlinked control with surface motility. BMC Microbiology. 2015; 15: 58.
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Tariq M, Lum MR, Chong AW, Amirapu AB, Hameed S, Hirsch AM. A reliable method for the selection and confirmation of transconjugants of plant growth-promoting bacteria especially plant-associated Burkholderia spp. J. Microbiol. Methods. . 2015; 117: 49-53.
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Nagata M, Yamamoto N, Shigeyama T, Terasawa Y, Anai T, Sakai T, Inada S, Arima S, Hashiguchi M, Akashi R, Nakayama H, Ueno D, Hirsch AM, Suzuki A. Red/Far Red Light Controls Arbuscular Mycorrhizal Colonization via Jasmonic Acid and Strigolactone Signaling. Plant Cell Physiol. 2015; 56(11): 2100-2109.
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Estrada-de los Santos P, Rojas-Rojas FU, Tapia-García EY, Vásquez-Murrieta MS, Hirsch AM. To split or not to split: an opinion on dividing the genus Burkholderia. . Ann. Microbiol. 2015; .
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Nagata M, Yamamoto N, Miyamoto T, Shimomura A, Arima S, Hirsch AM, Suzuki A. Enhanced hyphal growth of arbuscular mycorrhizae by root exudates derived from high R/FR treated Lotus japonicus. Plant Signal. Behavior. 2016; 11(6): .
Maymon M, Martínez-Hidalgo P, Tran SS, Ice T, Craemer K, Anbarchian T, Sung T, Chou M, Hwang L, Fujishige NA, Ventosa J, Villella W, Sikorski J, Faull KF, Sanders ER. Hirsch AM. Mining the phytomicrobiome to understand how bacterial coinoculations enhance plant growth. Front. Plant Sci. . 2015; 6: 784.
Sanders ER, Moberg-Parker J, Hirsch AM, Lee PY, Shapiro C, Toma S, Levis-Fitzgerald M. Transforming laboratory education in the life sciences. . Microbe 2016; 11: 69-74.
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De Meyer SE, Briscoe L, Martínez-Hidalgo P, Agapakis CM, Estrada de-los Santos P, Seshadri R, Reeve W, Weinstock G, O’Hara G, Howieson J, Hirsch AM. Symbiotic Burkholderia species show diverse arrangements of nif/fix and nod genes, and lack typical high affinity cytochrome cbb3 oxidase genes. Mol. Plant-Microbe Interact. . 2016; .
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