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Los Angeles, CA 90095 247 BSRB
CAMPUS - 157005
Los Angeles, CA 90095
|Professor, Microbiology, Immunology & Molecular Genetics|
|Member, Immunity, Microbes & Molecular Pathogenesis GPB Home Area, Molecular Pharmacology GPB Home Area, Tumor Immunology Program at the Jonsson Comprehensive Cancer Center (JCCC)|
Genetic engineering and gene transfection of antibodies In 1982 the Morrison laboratory developed the methods for expressing antibody genes in lymphoid cells. This technology made it possible to identify lymphoid specific controlling elements(enhancers) and to produce genetically engineered antibodies in the appropriate cellular environment. This approach made possible a systematic analysis of the structure/function relationships within the Ab molecule including an analysis of the role of carbohydrate in antibody function. It also made it possible to make novel antibodies and antibody based therapeutics.
De Oliveira Satiro N, Wang Jiexin, Ryan Christine, Morrison Sherie L, Kohn Donald B, Hollis Roger P A CD19/Fc fusion protein for detection of anti-CD19 chimeric antigen receptors. Journal of translational medicine. 2013; 11(15): 23.
Trinh Kham R, Vasuthasawat Alex, Steward Kristopher K, Yamada Reiko E, Timmerman John M, Morrison Sherie L Anti-CD20-interferon-Î² fusion protein therapy of murine B-cell lymphomas. Journal of immunotherapy (Hagerstown, Md. : 1997). 2013; 36(5): 305-18.
Asai Tsuneaki, Morrison Sherie L The SRC family tyrosine kinase HCK and the ETS family transcription factors SPIB and EHF regulate transcytosis across a human follicle-associated epithelium model. The Journal of biological chemistry. 2013; 288(15): 10395-405.
Yoo Esther M, Trinh K Ryan, Lim Hana, Wims Letitia A, Morrison Sherie L Characterization of IgA and IgM binding and internalization by surface-expressed human FcÎ±/Î¼ receptor. Molecular immunology. 2011; 48(15-16): 1818-26.
Yoo Esther M, Yu Li J, Wims Letitia A, Goldberg David, Morrison Sherie L Differences in N-glycan structures found on recombinant IgA1 and IgA2 produced in murine myeloma and CHO cell lines. mAbs. 2011; 2(3): 320-34.
Kim Sanggu, Kim Yun-Cheol, Qi Hangfei, Su Kunkai, Morrison Sherie L, Chow Samson A Efficient identification of human immunodeficiency virus type 1 mutants resistant to a protease inhibitor by using a random mutant library. Antimicrobial agents and chemotherapy. 2011; 55(11): 5090-8.
Mershon-Shier Kileen L, Vasuthasawat Alex, Takahashi Kazue, Morrison Sherie L, Beenhouwer David O In vitro C3 deposition on Cryptococcus capsule occurs via multiple complement activation pathways. Molecular immunology. 2011; 48(15-16): 2009-18.
Xuan Caiyun, Steward Kristopher K, Timmerman John M, Morrison Sherie L Targeted delivery of interferon-alpha via fusion to anti-CD20 results in potent antitumor activity against B-cell lymphoma. Blood. 2010; 115(14): 2864-71.
Kim Yun-Cheol, Morrison Sherie L A rapid and economic in-house DNA purification method using glass syringe filters. PloS one. 2009; 4(11): e7750.
Afshar Sepideh, Olafsen Tove, Wu Anna M, Morrison Sherie L Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT. Journal of experimental & clinical cancer research : CR. 2009; 28(14): 147.
Afshar Sepideh, Asai Tsuneaki, Morrison Sherie L Humanized ADEPT comprised of an engineered human purine nucleoside phosphorylase and a tumor targeting peptide for treatment of cancer. Molecular cancer therapeutics. 2009; 8(1): 185-93.
Cho John S, Kim Yun C, Morrison Sherie L Inhibitors of MyD88-dependent proinflammatory cytokine production identified utilizing a novel RNA interference screening approach. PloS one. 2009; 4(9): e7029.
Cho John S, Hsu Jeffrey V, Morrison Sherie L Localized expression of GITR-L in the tumor microenvironment promotes CD8+ T cell dependent anti-tumor immunity. Cancer immunology, immunotherapy : CII. 2009; 58(7): 1057-69.
Kim Yun Cheol, Morrison Sherie L N-terminal domain-deleted mu transposase exhibits increased transposition activity with low target site preference in modified buffers. Journal of molecular microbiology and biotechnology. 2009; 17(1): 30-40.
Montano Ramon F, Penichet Manuel L, Blackall Douglas P, Morrison Sherie L, Chintalacharuvu Koteswara R Recombinant polymeric IgG anti-Rh: a novel strategy for development of direct agglutinating reagents. Journal of immunological methods. 2009; 340(1): 1-10.
Mershon Kileen L, Vasuthasawat Alex, Lawson Gregory W, Morrison Sherie L, Beenhouwer David O Role of complement in protection against Cryptococcus gattii infection. Infection and immunity. 2009; 77(3): 1061-70.
Afshar Sepideh, Sawaya Michael R, Morrison Sherie L Structure of a mutant human purine nucleoside phosphorylase with the prodrug, 2-fluoro-2'-deoxyadenosine and the cytotoxic drug, 2-fluoroadenine. Protein science : a publication of the Protein Society. 2009; 18(5): 1107-14.
Kim Yun Cheol, Lee Hui Sun, Yoon Sukjoon, Morrison Sherie L Transposon-directed base-exchange mutagenesis (TDEM): a novel method for multiple-nucleotide substitutions within a target gene. BioTechniques. 2009; 46(7): 534-42.
Cheng Lucy S, Hwang Seungmin, Huang Tzu-Hsuan, Sheu Ting-Chang, Sun Ren, Morrison Sherie L, Cheng Genhong, Shahangian Arash Antibody-fused interferons as an effective approach to enhance target specificity and antiviral efficacy of type I interferons. Cell research. 2008; 18(12): 1230-2.
Aslam Akhmed, Quinn Phyllis, McIntosh Richard S, Shi Jianguo, Ghumra Ashfaq, McKerrow James H, Bunting Karen A, Dunne David W, Doenhoff Michael J, Morrison Sherie L, Zhang Ke, Pleass Richard J Proteases from Schistosoma mansoni cercariae cleave IgE at solvent exposed interdomain regions. Molecular immunology. 2008; 45(2): 567-74.
Beenhouwer David O, Yoo Esther M, Lai Chun-Wei, Rocha Miguel A, Morrison Sherie L Human immunoglobulin G2 (IgG2) and IgG4, but not IgG1 or IgG3, protect mice against Cryptococcus neoformans infection. Infection and immunity. 2007; 75(3): 1424-35.
Chintalacharuvu Koteswara R, Gurbaxani Brian, Morrison Sherie L Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells. Molecular immunology. 2007; 44(13): 3445-52.
Huang Tzu-Hsuan, Chintalacharuvu Koteswara R, Morrison Sherie L Targeting IFN-alpha to B cell lymphoma by a tumor-specific antibody elicits potent antitumor activities. Journal of immunology (Baltimore, Md. : 1950). 2007; 179(10): 6881-8.
Olafsen Tove, Gu Zhennan, Sherman Mark A, Leyton Jeffrey V, Witkosky Michael E, Shively John E, Raubitschek Andrew A, Morrison Sherie L, Wu Anna M, Reiter Robert E Targeting, imaging, and therapy using a humanized antiprostate stem cell antigen (PSCA) antibody. Journal of immunotherapy (Hagerstown, Md. : 1997). 2007; 30(4): 396-405.
Morrison Sherie L Two heads are better than one. Nature biotechnology. 2007; 25(11): 1233-4.
Huang Tzu-Hsuan, Morrison Sherie L A trimeric anti-HER2/neu ScFv and tumor necrosis factor-alpha fusion protein induces HER2/neu signaling and facilitates repair of injured epithelia. The Journal of pharmacology and experimental therapeutics. 2006; 316(3): 983-91.
Gurbaxani Brian, Dela Cruz Linh L, Chintalacharuvu Koteswara, Morrison Sherie L Analysis of a family of antibodies with different half-lives in mice fails to find a correlation between affinity for FcRn and serum half-life. Molecular immunology. 2006; 43(9): 1462-73.
Dela Cruz Jay Soriano, Trinh Kamh Ryan, Chen Hsiao Wen, Ribas Antoni, Morrison Sherie L, Penichet Manuel L Anti-HER2/neu IgG3-(IL-2) and anti-HER2/neu IgG3-(GM-CSF) promote HER2/neu processing and presentation by dendritic cells: implications in immunotherapy and vaccination strategies. Molecular immunology. 2006; 43(6): 667-76.
Gurbaxani Brian Mohan, Morrison Sherie L Development of new models for the analysis of Fc-FcRn interactions. Molecular immunology. 2006; 43(9): 1379-89.
Ng Patrick P, Helguera Gustavo, Daniels Tracy R, Lomas Simon Z, Rodriguez Jose A, Schiller Gary, Bonavida Benjamin, Morrison Sherie L, Penichet Manuel L Molecular events contributing to cell death in malignant human hematopoietic cells elicited by an IgG3-avidin fusion protein targeting the transferrin receptor. Blood. 2006; 108(8): 2745-54.
Samuelsson Martin, Jendholm Johan, Amisten Stefan, Morrison Sherie L, Forsgren Arne, Riesbeck Kristian The IgD CH1 region contains the binding site for the human respiratory pathogen Moraxella catarrhalis IgD-binding protein MID. European journal of immunology. 2006; 36(9): 2525-34.
Helguera Gustavo, Dela Cruz Jay S, Lowe Christine, Ng Patrick P, Trinh Ryan, Morrison Sherie L, Penichet Manuel L Vaccination with novel combinations of anti-HER2/neu cytokines fusion proteins and soluble protein antigen elicits a protective immune response against HER2/neu expressing tumors. Vaccine. 2006; 24(3): 304-16.
Asai Tsuneaki, Trinh Ryan, Ng Patrick P, Penichet Manuel L, Wims Letitia A, Morrison Sherie L A human biotin acceptor domain allows site-specific conjugation of an enzyme to an antibody-avidin fusion protein for targeted drug delivery. Biomolecular engineering. 2005; 21(6): 145-55.
Asai Tsuneaki, Wims Letitia A, Morrison Sherie L An interaction between S*tag and S*protein derived from human ribonuclease 1 allows site-specific conjugation of an enzyme to an antibody for targeted drug delivery. Journal of immunological methods. 2005; 299(1-2): 63-76.
Dela Cruz JS, Trinh KR, Chen HW, Ribas A, Morrison S L, Penichet ML Anti-HER2/neu IgG3-(IL-2) and anti-HER2/neu IgG3-(GM-CSF) promote HER2/neu processing and presentation by dendritic cells: Implications in immunotherapy and vaccination strategies. Mol Immunol. 2005; .
Cho Hyun-Mi, Rosenblatt Joseph D, Kang Young-Sook, Iruela-Arispe M Luisa, Morrison Sherie L, Penichet Manuel L, Kwon Young-Guen, Kim Tae-Woong, Webster Keith A, Nechustan Hovav, Shin Seung-Uon Enhanced inhibition of murine tumor and human breast tumor xenografts using targeted delivery of an antibody-endostatin fusion protein. Molecular cancer therapeutics. 2005; 4(6): 956-67.
Yoo Esther M, Morrison Sherie L IgA: an immune glycoprotein. Clinical immunology (Orlando, Fla.). 2005; 116(1): 3-10.
Dela Cruz Jay S, Morrison Sherie L, Penichet Manuel L Insights into the mechanism of anti-tumor immunity in mice vaccinated with the human HER2/neu extracellular domain plus anti-HER2/neu IgG3-(IL-2) or anti-HER2/neu IgG3-(GM-CSF) fusion protein. Vaccine. 2005; 23(39): 4793-803.
Zhu Lei, van de Lavoir Marie-Cecile, Albanese Jenny, Beenhouwer David O, Cardarelli Pina M, Cuison Severino, Deng David F, Deshpande Shrikant, Diamond Jennifer H, Green Lynae, Halk Edward L, Heyer Babette S, Kay Robert M, Kerchner Allyn, Leighton Philip A, Mather Christine M, Morrison Sherie L, Nikolov Zivko L, Passmore David B, Pradas-Monne Alicia, Preston Benjamin T, Rangan Vangipuram S, Shi Mingxia, Srinivasan Mohan, White Steven G, Winters-Digiacinto Peggy, Wong Susan, Zhou Wen, Etches Robert J Production of human monoclonal antibody in eggs of chimeric chickens. Nature biotechnology. 2005; 23(9): 1159-69.
Dela Cruz JS, Huang TH, Penichet ML, Morrison SL Antibody-cytokine fusion proteins: innovative weapons in the war against cancer. Clinical and experimental medicine. . 2004; 4(2): 57-64.
Trinh Ryan, Gurbaxani Brian, Morrison Sherie L, Seyfzadeh Manouchehr Optimization of codon pair use within the (GGGGS)3 linker sequence results in enhanced protein expression. Molecular immunology. 2004; 40(10): 717-22.
Gala FranÃ§oise A, Morrison Sherie L V region carbohydrate and antibody expression. Journal of immunology (Baltimore, Md. : 1950). 2004; 172(9): 5489-94.
Chan Lisa A, Phillips Martin L, Wims Letitia A, Trinh K Ryan, Denham Jerrod, Morrison Sherie L Variable region domain exchange in human IgGs promotes antibody complex formation with accompanying structural changes and altered effector functions. Molecular immunology. 2004; 41(5): 527-38.
Chintalacharuvu Koteswara R, Chuang Philip D, Dragoman Ashley, Fernandez Christine Z, Qiu Jiazhou, Plaut Andrew G, Trinh K Ryan, Gala FranÃ§oise A, Morrison Sherie L Cleavage of the human immunoglobulin A1 (IgA1) hinge region by IgA1 proteases requires structures in the Fc region of IgA. Infection and immunity. 2003; 71(5): 2563-70.
Yoo Esther M, Wims Letitia A, Chan Lisa A, Morrison Sherie L Human IgG2 can form covalent dimers. Journal of immunology (Baltimore, Md. : 1950). 2003; 170(6): 3134-8.
Dela Cruz Jay S, Lau Suk Ying, Ramirez Ernesto M, De Giovanni Carla, Forni Guido, Morrison Sherie L, Penichet Manuel L Protein vaccination with the HER2/neu extracellular domain plus anti-HER2/neu antibody-cytokine fusion proteins induces a protective anti-HER2/neu immune response in mice. Vaccine. 2003; 21(13-14): 1317-26.
Ng Patrick P, Dela Cruz Jay S, Sorour David N, Stinebaugh James M, Shin Seung-Uon, Shin Daniel S, Morrison Sherie L, Penichet Manuel L An anti-transferrin receptor-avidin fusion protein exhibits both strong proapoptotic activity and the ability to deliver various molecules into cancer cells. Proceedings of the National Academy of Sciences of the United States of America. 2002; 99(16): 10706-11.
Westin Kwon Katherine, Lendvai Nikoletta, Morrison Sherie, Trinh K Ryan, Casadevall Arturo Biological activity of a mouse-human chimeric immunoglobulin G2 antibody to Cryptococcus neoformans polysaccharide. Clinical and diagnostic laboratory immunology. 2002; 9(1): 201-4.
Morrison Sherie L Cloning, expression, and modification of antibody V regions. Current protocols in immunology / edited by John E. Coligan ... [et al.]. 2002; Chapter 2(16): Unit 2.12.
Chintalacharuvu Koteswara R, Yu Li J, Bhola Nishant, Kobayashi Kunihiko, Fernandez Christine Z, Morrison Sherie L Cysteine residues required for the attachment of the light chain in human IgA2. Journal of immunology (Baltimore, Md. : 1950). 2002; 169(9): 5072-7.
MontaÃ±o Ramon F, Morrison Sherie L Influence of the isotype of the light chain on the properties of IgG. Journal of immunology (Baltimore, Md. : 1950). 2002; 168(1): 224-31.
Yoo Esther M, Chintalacharuvu Koteswara R, Penichet Manuel L, Morrison Sherie L Myeloma expression systems. Journal of immunological methods. 2002; 261(1-2): 1-20.
Morrison Sherie L, Mohammed Mansoor S, Wims Letitia A, Trinh Ryan, Etches Robert Sequences in antibody molecules important for receptor-mediated transport into the chicken egg yolk. Molecular immunology. 2002; 38(8): 619-25.
Montano RF, Morrison SL The isotype of the light chain can influence the properties of IgG. J. Immunol 2002; 168: 224-231.
Gala Francoise A, Morrison Sherie L The role of constant region carbohydrate in the assembly and secretion of human IgD and IgA1. The Journal of biological chemistry. 2002; 277(32): 29005-11.
Penichet ML, Dela Cruz JS, Challita-Eid PM, Rosenblatt JD, Morrison SL A murine B cell lymphoma expressing human HER2 / neu undergoes spontaneous tumor regression and elicits antitumor immunity. Cancer Immunol Immunother. 2001; 49(12): 649-62.
Penichet ML, Dela Cruz JS, Shin SU, Morrison SL A recombinant IgG3-(IL-2) fusion protein for the treatment of human HER2/neu expressing tumors. Hum Antibodies. 2001; 10(1): 43-9.
Penichet ML, Morrison SL Antibody-cytokine fusion proteins for the therapy of cancer. J Immunol Methods. 2001; 248(1-2): 91-101.
Chintalacharuvu KR, Vuong LU, Loi LA, Larrick JW, Morrison SL Hybrid IgA2/IgG1 antibodies with tailor-made effector functions. Clinical immunology (Orlando, Fla.) . 2001; 101(1): 21-31.
Peng LS, Penichet ML, Dela Cruz JS, Sampogna SL, Morrison SL Mechanism of antitumor activity of a single-chain interleukin-12 IgG3 antibody fusion protein (mscIL-12.her2.IgG3). Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. . 2001; 21(9): 709-20.
Zhang H, Lu S, Morrison SL, Tomlinson S Targeting of functional antibody-decay-accelerating factor fusion proteins to a cell surface. J Biol Chem. 2001; 276(29): 27290-5.
Wright A, Sato Y, Okada T, Chang K, Endo T, Morrison S In vivo trafficking and catabolism of IgG1 antibodies with Fc associated carbohydrates of differing structure. Glycobiology. . 2000; 10(12): 1347-55.
Dela Cruz JS, Trinh KR, Morrison SL, Penichet ML Recombinant anti-human HER2/neu IgG3-(GM-CSF) fusion protein retains antigen specificity, cytokine function and demonstrates anti-tumor activity. J. Immunol 2000; 165: 5112-5121.
Mateo C, Lombardero J, Moreno E, Morales A, Bombino G, Coloma J, Wims L, Morrison SL, Perez R Removal of amphipathic epitopes from genetically engineered antibodies: production of modified immunoglobulins with reduced immunogenicity. Hybridoma. 2000; 19(6): 463-71.
Trinh KR, Morrison SL Site-specific and directional gene replacement mediated by Cre recombinase. Journal of immunological methods. . 2000; 244(1-2): 185-93.
Rifai A, Fadden K, Morrison SL, Chintalacharuvu KR The N-glycans determine the differential blood clearance and hepatic uptake of human immunoglobulin (Ig)A1 and IgA2 isotypes. The Journal of experimental medicine. . 2000; 191(12): 2171-82.
Chan LA, Lyczak JB, Zhang K, Morrison SL, Saxon A The novel human IgE epsilon heavy chain, epsilon tailpiece, is present in plasma as part of a covalent complex. Molecular immunology. . 2000; 37(5): 241-52.
Coloma MJ, Clift A, Wims L, Morrison SL The role of carbohydrate in the assembly and function of polymeric IgG. Molecular immunology. . 2000; 37(18): 1081-90.
Coloma MJ, Lee HJ, Kurihara A, Landaw EM, Boado RJ, Morrison SL, Pardridge WM Transport across the primate blood-brain barrier of a genetically engineered chimeric monoclonal antibody to the human insulin receptor. Pharmaceutical research. . 2000; 17(3): 266-74.
Peng LS, Penichet ML, Morrison SL A single-chain IL-12 IgG3 antibody fusion protein retains antibody specificity and IL-12 bioactivity and demonstrates antitumor activity. Journal of immunology (Baltimore, Md. : 1950) . 1999; 163(1): 250-8.
Penichet ML, Kang YS, Pardridge WM, Morrison SL, Shin SU An antibody-avidin fusion protein specific for the transferrin receptor serves as a delivery vehicle for effective brain targeting: initial applications in anti-HIV antisense drug delivery to the brain. J Immunol. 1999; 163(8): 4421-6.
Berson AE, Young C, Morrison SL, Fujii GH, Sheung J, Wu B, Bolen JB, Burkhardt AL Identification and characterization of a myristylated and palmitylated serine/threonine protein kinase. Biochemical and biophysical research communications. . 1999; 259(3): 533-8.
Penichet ML, Challita PM, Shin SU, Sampogna SL, Rosenblatt JD, Morrison SL In vivo properties of three human HER2/neu-expressing murine cell lines in immunocompetent mice. Lab Anim Sci. 1999; 49(2): 179-88.
Coloma MJ, Trinh RK, Martinez AR, Morrison SL Position effects of variable region carbohydrate on the affinity and in vivo behavior of an anti-(1-->6) dextran antibody. J Immunol. 1999; 162(4): 2162-70.
Chintalacharuvu KR, Morrison SL Production and characterization of recombinant IgA. Immunotechnology. 1999; 4(3-4): 165-74.
Yoo EM, Coloma MJ, Trinh KR, Nguyen TQ, Vuong LU, Morrison SL, Chintalacharuvu KR Structural requirements for polymeric immunoglobulin assembly and association with J chain. The Journal of biological chemistry. . 1999; 274(47): 33771-7.
Zhang HF, Yu J, Bajwa E, Morrison SL, Tomlinson S Targeting of functional antibody-CD59 fusion proteins to a cell surface. J Clin Invest. 1999; 103(1): 55-61.
Bonagura VR, Kwong T, Kenny T, Robbins DL, Morrison SL The specificity of synovial IgM rheumatoid factors (RF) for genetically engineered IgG antibodies is not affected by the method used to immortalize RF-producing B cells. Scand J Immunol. 1999; 49(1): 106-11.
Wright A and Morrison SL Effect of CH2-associated carbohydrate structure on Ig effector function: Studies with chimeric mouse-human IgG1 antibodies in glycosylation mutants of Chinese hamster ovary cells. J. Immunol 1998; 160: 3393-3402.
Chintalacharuvu KW and Morrison SL . Production of secretory immunoglobulin A by a single mammalian cell. Proc. Natl. Acad. Sci 1997; 94: 6364-6368.
Smith RIF, Coloma MJ and Morrison SL Addition of a m-tailpiece to IgG Results in Polymeric Antibodies with Enhanced Effector Functions Including Complement Mediated. J. Immunol 1995; 154: 2226-2236.
Yamada Douglas H, Elsaesser Heidi, Lux Anja, Timmerman John M, Morrison Sherie L, de la Torre Juan Carlos, Nimmerjahn Falk, Brooks David G Suppression of FcÎ³-receptor-mediated antibody effector function during persistent viral infection. Immunity. 2015; 42(2): 379-90.
Young Patricia A, Morrison Sherie L, Timmerman John M Antibody-cytokine fusion proteins for treatment of cancer: engineering cytokines for improved efficacy and safety. Seminars in oncology. 2014; 41(5): 623-36.
Vasuthasawat Alex, Yoo Esther M, Trinh Kham R, Lichtenstein Alan, Timmerman John M, Morrison Sherie L Targeted immunotherapy using anti-CD138-interferon Î± fusion proteins and bortezomib results in synergistic protection against multiple myeloma. mAbs. 2016; 1-12.
Yoo Esther M, Trinh Kham R, Tran Danh, Vasuthasawat Alex, Zhang Juan, Hoang Bao, Lichtenstein Alan, Morrison Sherie L Anti-CD138-targeted interferon is a potent therapeutic against multiple myeloma. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. 2015; 35(4): 281-91.