Emil Reisler, Ph.D.

Laboratory Address:
Paul Boyer Hall 405

Mailing Address:
Mail Code: 143801
Los Angeles, CA 90095

Work Address:
Paul Boyer Hall 401A

Affiliations
Affiliations
Distinguished Professor, Chemistry and Biochemistry, Biochemistry, Molecular Biology
Lab Director, Reisler Lab
Member, Graduate Program in Biochemistry, Molecular and Structural Biology, Molecular Biology Institute
Researcher, Biochemistry, Biophysics and Structural Biology, Proteomics and Bioinformatics
Research Interests
Work done by muscle and non-muscle cells requires the transduction of chemical into mechanical energy. This function is carried out by actin, tubulin, and a family of motor proteins. The interactions between these proteins and the changes in their structure constitute the molecular basis for force generation and motility in muscle and non-muscle cells. Our goal of elucidating the mechanism of contractile processes is pursued at two levels. At the molecular level, we are concerned with the structural and dynamic properties of the contractile proteins. We explore structure-function relationships in actin, myosin and other proteins by biochemical, biophysical, immunochemical, mutational, and in the vitro motility and force measurements. Intermediate steps in the contractile process are probed with the help of nucleotide analogues, specific antibodies, synthetic peptides, and appropriate mutants of the key proteins. The aim of these studies is to obtain a structural description of the mechanism of motion and force generation by motor proteins., At the cellular level, we study the function, interactions, and structural transitions of the assembled protein systems. Many filamentous cellular structures are constructed from their components through fine-tuned assembly processes. The biological function of these filaments is frequently linked to the signaling and the regulation of assembly by cellular factors. Our interest is focused on the formation and remodeling of actin filaments, bundles, and networks by actin binding proteins. The mechanisms of the assembly reactions and the structural changes which govern them are studied by biophysical, biochemical, and electron microscopy methods. To understand the regulation of actin assembly by other proteins, macromolecular contact sites are investigated by a wide spectrum of experimental techniques.
Biography:

Dr. Reisler received his B.A. from the Hebrew University in Jerusalem, Israel and his Ph.D. from the Weizmann Institute of Science in Rehovot, Israel. He was a Postdoctoral Scholar in the Biology Department of the Johns Hopkins University.

Publications
Yehl Jenna, Kudryashova Elena, Reisler Emil, Kudryashov Dmitri, Polenova Tatyana Structural Analysis of Human Cofilin 2/Filamentous Actin Assemblies: Atomic-Resolution Insights from Magic Angle Spinning NMR Spectroscopy. Scientific reports. 2017; 7: 44506.
Grintsevich Elena E, Yesilyurt Hunkar Gizem, Rich Shannon K, Hung Ruei-Jiun, Terman Jonathan R, Reisler Emil F-actin dismantling through a redox-driven synergy between Mical and cofilin. Nature cell biology. 2016; 18(8): 876-85.
Mikati Mouna A, Breitsprecher Dennis, Jansen Silvia, Reisler Emil, Goode Bruce L Coronin Enhances Actin Filament Severing by Recruiting Cofilin to Filament Sides and Altering F-Actin Conformation. Journal of molecular biology. 2015; 427(19): 3137-47.
Oztug Durer Zeynep A, McGillivary Rebecca M, Kang Hyeran, Elam W Austin, Vizcarra Christina L, Hanein Dorit, De La Cruz Enrique M, Reisler Emil, Quinlan Margot E Metavinculin Tunes the Flexibility and the Architecture of Vinculin-Induced Bundles of Actin Filaments. Journal of molecular biology. 2015; 427(17): 2782-98.
Ge Peng, Durer Zeynep A Oztug, Kudryashov Dmitri, Zhou Z Hong, Reisler Emil Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments. Nature structural & molecular biology. 2014; 21(12): 1075-81.
Grintsevich Elena E, Reisler Emil Drebrin inhibits cofilin-induced severing of F-actin. Cytoskeleton (Hoboken, N.J.). 2014; 71(8): 472-83.
Sharma Shivani, Grintsevich Elena E, Woo JungReem, Gurel Pinar S, Higgs Henry N, Reisler Emil, Gimzewski James K Nanostructured self-assembly of inverted formin 2 (INF2) and F-actin-INF2 complexes revealed by atomic force microscopy. Langmuir : the ACS journal of surfaces and colloids. 2014; 30(25): 7533-9.
Gurel Pinar S, Ge Peng, Grintsevich Elena E, Shu Rui, Blanchoin Laurent, Zhou Z Hong, Reisler Emil, Higgs Henry N INF2-mediated severing through actin filament encirclement and disruption. Current biology : CB. 2014; 24(2): 156-64.
Chen Christine K, Benchaar Sabrina Amelle, Phan Mai, Grintsevich Elena E, Loo Rachel R Ogorzalek, Loo Joseph A, Reisler Emil Cofilin-induced changes in F-actin detected via cross-linking with benzophenone-4-maleimide. Biochemistry. 2013; .
Sharma Shivani, Zhu Huanqi, Grintsevich Elena E, Reisler Emil, Gimzewski James K Correlative nanoscale imaging of actin filaments and their complexes. Nanoscale. 2013; 5(13): 5692-702.
Mikati Mouna A, Grintsevich Elena E, Reisler Emil Drebrin-induced Stabilization of Actin Filaments. The Journal of biological chemistry. 2013; 288(27): 19926-38.
Kudryashov Dmitri S, Reisler Emil ATP and ADP actin states. Biopolymers. 2013; 99(4): 245-56.
Kang Hyeran, Bradley Michael J, McCullough Brannon R, Pierre Anaëlle, Grintsevich Elena E, Reisler Emil, De La Cruz Enrique M Identification of cation-binding sites on actin that drive polymerization and modulate bending stiffness. Proceedings of the National Academy of Sciences of the United States of America. 2012; 109(42): 16923-7.
Durer Zeynep A Oztug, Kudryashov Dmitri S, Sawaya Michael R, Altenbach Christian, Hubbell Wayne, Reisler Emil Structural states and dynamics of the D-loop in actin. Biophysical journal. 2012; 103(5): 930-9.
Sharma Shivani, Grintsevich Elena E, Hsueh Carlin, Reisler Emil, Gimzewski James K Molecular cooperativity of drebrin1-300 binding and structural remodeling of F-actin. Biophysical journal. 2012; 103(2): 275-83.
Chen Christine K, Sawaya Michael R, Phillips Martin L, Reisler Emil, Quinlan Margot E Multiple forms of Spire-actin complexes and their functional consequences. The Journal of biological chemistry. 2012; 287(13): 10684-92.
Galkin Vitold E, Orlova Albina, Kudryashov Dmitri S, Solodukhin Alexander, Reisler Emil, Schröder Gunnar F, Egelman Edward H Remodeling of actin filaments by ADF/cofilin proteins. Proceedings of the National Academy of Sciences of the United States of America. 2011; 108(51): 20568-72.
Oztug Durer Zeynep A, Kamal J K Amisha, Benchaar Sabrina, Chance Mark R, Reisler Emil Myosin binding surface on actin probed by hydroxyl radical footprinting and site-directed labels. Journal of molecular biology. 2011; 414(2): 204-16.
McCullough Brannon R, Grintsevich Elena E, Chen Christine K, Kang Hyeran, Hutchison Alan L, Henn Arnon, Cao Wenxiang, Suarez Cristian, Martiel Jean-Louis, Blanchoin Laurent, Reisler Emil, De La Cruz Enrique M Cofilin-linked changes in actin filament flexibility promote severing. Biophysical journal. 2011; 101(1): 151-9.
Sharma Shivani, Grintsevich Elena E, Phillips Martin L, Reisler Emil, Gimzewski James K Atomic force microscopy reveals drebrin induced remodeling of f-actin with subnanometer resolution. Nano letters. 2011; 11(2): 825-7.
Muhlrad Andras, Grintsevich Elena E, Reisler Emil Polycation induced actin bundles. Biophysical chemistry. 2011; .
Kudryashov Dmitri S, Grintsevich Elena E, Rubenstein Peter A, Reisler Emil A nucleotide state-sensing region on actin. The Journal of biological chemistry. 2010; 285(33): 25591-601.
Grintsevich Elena E, Phillips Martin, Pavlov Dmitry, Phan Mai, Reisler Emil, Muhlrad Andras Antiparallel dimer and actin assembly. Biochemistry. 2010; 49(18): 3919-27.
Grintsevich Elena E, Galkin Vitold E, Orlova Albina, Ytterberg A Jimmy, Mikati Mouna M, Kudryashov Dmitri S, Loo Joseph A, Egelman Edward H, Reisler Emil Mapping of drebrin binding site on F-actin. Journal of molecular biology. 2010; 398(4): 542-54.
Oztug Durer Zeynep A, Diraviyam Karthikeyan, Sept David, Kudryashov Dmitri S, Reisler Emil F-actin structure destabilization and DNase I binding loop: fluctuations mutational cross-linking and electron microscopy analysis of loop states and effects on F-actin. Journal of molecular biology. 2010; 395(3): 544-57.
Shvetsov Alexander, Berkane Emir, Chereau David, Dominguez Roberto, Reisler Emil The actin-binding domain of cortactin is dynamic and unstructured and affects lateral and longitudinal contacts in F-actin. Cell motility and the cytoskeleton. 2009; 66(2): 90-8.
Scoville Damon, Stamm John D, Altenbach Christian, Shvetsov Alexander, Kokabi Kaveh, Rubenstein Peter A, Hubbell Wayne L, Reisler Emil Effects of binding factors on structural elements in F-actin. Biochemistry. 2009; 48(2): 370-8.
Kudryashov Dmitri S, Durer Zeynep A Oztug, Ytterberg A Jimmy, Sawaya Michael R, Pashkov Inna, Prochazkova Katerina, Yeates Todd O, Loo Rachel R Ogorzalek, Loo Joseph A, Satchell Karla J Fullner, Reisler Emil Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin. Proceedings of the National Academy of Sciences of the United States of America. 2008; 105(47): 18537-42.
Sawaya Michael R, Kudryashov D S, Pashkov Inna, Adisetiyo Helty, Reisler Emil, Yeates Todd O Multiple crystal structures of actin dimers and their implications for interactions in the actin filament. Acta crystallographica. Section D, Biological crystallography. 2008; 64(Pt 4): 454-65.
Grintsevich Elena E, Benchaar Sabrina A, Warshaviak Dora, Boontheung Pinmanee, Halgand Frédéric, Whitelegge Julian P, Faull Kym F, Loo Rachel R Ogorzalek, Sept David, Loo Joseph A, Reisler Emil Mapping the cofilin binding site on yeast G-actin by chemical cross-linking. Journal of molecular biology. 2008; 377(2): 395-409.
Shvetsov Alexander, Galkin Vitold E, Orlova Albina, Phillips Martin, Bergeron Sarah E, Rubenstein Peter A, Egelman Edward H, Reisler Emil Actin hydrophobic loop 262-274 and filament nucleation and elongation. Journal of molecular biology. 2008; 375(3): 793-801.
Kudryashov Dmitri S, Cordero Christina L, Reisler Emil, Satchell Karla J Fullner Characterization of the enzymatic activity of the actin cross-linking domain from the Vibrio cholerae MARTX Vc toxin. The Journal of biological chemistry. 2008; 283(1): 445-52.
Reisler Emil, Egelman Edward H Actin structure and function: what we still do not understand. The Journal of biological chemistry. 2007; 282(50): 36133-7.
Kamal J K Amisha, Benchaar Sabrina A, Takamoto Keiji, Reisler Emil, Chance Mark R Three-dimensional structure of cofilin bound to monomeric actin derived by structural mass spectrometry data. Proceedings of the National Academy of Sciences of the United States of America. 2007; 104(19): 7910-5.
Pavlov Dmitry, Muhlrad Andras, Cooper John, Wear Martin, Reisler Emil Actin filament severing by cofilin. Journal of molecular biology. 2007; 365(5): 1350-8.
Benchaar Sabrina A, Xie Yongming, Phillips Martin, Loo Rachel R Ogorzalek, Galkin Vitold E, Orlova Albina, Thevis Mario, Muhlrad Andras, Almo Steven C, Loo Joseph A, Egelman Edward H, Reisler Emil Mapping the interaction of cofilin with subdomain 2 on actin. Biochemistry. 2007; 46(1): 225-33.
Muhlrad Andras, Ringel Israel, Pavlov Dmitry, Peyser Y Michael, Reisler Emil Antagonistic effects of cofilin, beryllium fluoride complex, and phalloidin on subdomain 2 and nucleotide-binding cleft in F-actin. Biophysical journal. 2006; 91(12): 4490-9.
Scoville Damon, Stamm John D, Toledo-Warshaviak Dora, Altenbach Christian, Phillips Martin, Shvetsov Alexander, Rubenstein Peter A, Hubbell Wayne L, Reisler Emil Hydrophobic loop dynamics and actin filament stability. Biochemistry. 2006; 45(45): 13576-84.
Sowa Glenna Z, Cannell David S, Liu Andrea J, Reisler Emil Polyamine-induced bundling of F-actin. The journal of physical chemistry. B. 2006; 110(44): 22279-84.
Cordero Christina L, Kudryashov Dmitry S, Reisler Emil, Satchell Karla J Fullner The Actin cross-linking domain of the Vibrio cholerae RTX toxin directly catalyzes the covalent cross-linking of actin. The Journal of biological chemistry. 2006; 281(43): 32366-74.
Pavlov Dmitry, Muhlrad Andras, Cooper John, Wear Martin, Reisler Emil Severing of F-actin by yeast cofilin is pH-independent. Cell motility and the cytoskeleton. 2006; 63(9): 533-42.
Kudryashov D S, Galkin V E, Orlova A, Phan M, Egelman E H, Reisler E Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface. Journal of molecular biology. 2006; 358(3): 785-97.
Shvetsov Alexander, Stamm John D, Phillips Martin, Warshaviak Dora, Altenbach Christian, Rubenstein Peter A, Hideg Kálmán, Hubbell Wayne L, Reisler Emil Conformational dynamics of loop 262-274 in G- and F-actin. Biochemistry. 2006; 45(20): 6541-9.
Muhlrad Andras, Pavlov Dmitry, Peyser Y Michael, Reisler Emil Inorganic phosphate regulates the binding of cofilin to actin filaments. The FEBS journal. 2006; 273(7): 1488-96.
Bobkov Andrey A, Muhlrad Andras, Pavlov Dmitry A, Kokabi Kaveh, Yilmaz Atilgan, Reisler Emil Cooperative effects of cofilin (ADF) on actin structure suggest allosteric mechanism of cofilin function. Journal of molecular biology. 2006; 356(2): 325-34.
Kudryashov Dmitry S, Sawaya Michael R, Adisetiyo Helty, Norcross Todd, Hegyi György, Reisler Emil, Yeates Todd O The crystal structure of a cross-linked actin dimer suggests a detailed molecular interface in F-actin. Proceedings of the National Academy of Sciences of the United States of America. 2005; 102(37): 13105-10.
Guan Jing-Qu, Takamoto Keiji, Almo Steven C, Reisler Emil, Chance Mark R Structure and dynamics of the actin filament. Biochemistry. 2005; 44(9): 3166-75.
Orlova Albina, Shvetsov Alexander, Galkin Vitold E, Kudryashov Dmitry S, Rubenstein Peter A, Egelman Edward H, Reisler Emil Actin-destabilizing factors disrupt filaments by means of a time reversal of polymerization. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101(51): 17664-8.
Muhlrad Andras, Kudryashov Dmitry, Michael Peyser Y, Bobkov Andrey A, Almo Steve C, Reisler Emil Cofilin induced conformational changes in F-actin expose subdomain 2 to proteolysis. Journal of molecular biology. 2004; 342(5): 1559-67.
Kudryashov Dmitry S, Phillips Martin, Reisler Emil Formation and destabilization of actin filaments with tetramethylrhodamine-modified actin. Biophysical journal. 2004; 87(2): 1136-45.
Bobkov Andrey A, Muhlrad Andras, Shvetsov Alexander, Benchaar Sabrina, Scoville Damon, Almo Steven C, Reisler Emil Cofilin (ADF) affects lateral contacts in F-actin. Journal of molecular biology. 2004; 337(1): 93-104.
Galkin Vitold E, Orlova Albina, VanLoock Margaret S, Shvetsov Alexander, Reisler Emil, Egelman Edward H ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments. The Journal of cell biology. 2003; 163(5): 1057-66.
Kudryashov Dmitry S, Reisler Emil Solution properties of tetramethylrhodamine-modified G-actin. Biophysical journal. 2003; 85(4): 2466-75.
Guan Jing-Qu, Almo Steven C, Reisler Emil, Chance Mark R Structural reorganization of proteins revealed by radiolysis and mass spectrometry: G-actin solution structure is divalent cation dependent. Biochemistry. 2003; 42(41): 11992-2000.
Nitao Lisa K, Loo Rachel R Ogorzalek, O'Neall-Hennessey Elizabeth, Loo Joseph A, Szent-Györgyi Andrew G, Reisler Emil Conformation and dynamics of the SH1-SH2 helix in scallop myosin. Biochemistry. 2003; 42(25): 7663-74.
Muhlrad Andras, Peyser Y Michael, Nili Mahta, Ajtai Katalin, Reisler Emil, Burghardt Thomas P Chemical decoupling of ATPase activation and force production from the contractile cycle in myosin by steric hindrance of lever-arm movement. Biophysical journal. 2003; 84(2 Pt 1): 1047-56.
Pavlov Dmitry, Gerson Jack H, Yu Tianwei, Tobacman Larry S, Homsher Earl, Reisler Emil The regulation of subtilisin-cleaved actin by tropomyosin/troponin. The Journal of biological chemistry. 2003; 278(8): 5517-22.
Nitao Lisa K, Yeates Todd O, Reisler Emil Conformational dynamics of the SH1-SH2 helix in the transition states of myosin subfragment-1. Biophysical journal. 2002; 83(5): 2733-41.
Bobkov Andrey A, Muhlrad Andras, Kokabi Kaveh, Vorobiev Sergey, Almo Steven C, Reisler Emil Structural effects of cofilin on longitudinal contacts in F-actin. Journal of molecular biology. 2002; 323(4): 739-50.
Wong Wenise W, Gerson Jack H, Rubenstein Peter A, Reisler Emil Thin filament regulation and ionic interactions between the N-terminal region in actin and troponin. Biophysical journal. 2002; 83(5): 2726-32.
Shvetsov Alexander, Musib Runa, Phillips Martin, Rubenstein Peter A, Reisler Emil Locking the hydrophobic loop 262-274 to G-actin surface by a disulfide bridge prevents filament formation. Biochemistry. 2002; 41(35): 10787-93.
Doyle, TC Reisler, E Insights into actomyosin interactions from actin mutations. Results and problems in cell differentiation. . 2002; 36: 31-49.
Kim Eldar, Bobkova Elena, Hegyi György, Muhlrad Andras, Reisler Emil Actin cross-linking and inhibition of the actomyosin motor. Biochemistry. 2002; 41(1): 86-93.
Doyle Timothy C, Reisler Emil Insights into actomyosin interactions from actin mutations. Results and problems in cell differentiation. 2002; 36(1): 31-49.