Luisa Iruela-Arispe, Ph.D.

Mailing Address:
BOX 951606, 621 Charles E. Young Dr. S,
Los Angeles, CA 90095

Work Address:
611 Charles E. Young Drive East
Paul Boyer 559
Los Angeles, CA 90095

Chair, Molecular Biology Interdepartmental Program
Director, Molecular Biology Institute
Professor and Vice Chair, Molecular, Cell, and Developmental Biology
Member, CTSI, Cell & Developmental Biology GPB Home Area, Genetics & Genomics GPB Home Area, Molecular, Cellular & Integrative Physiology GPB Home Area
Research Interests
Establishment of a vascular system is a key requirement for the development and survival of the mammalian embryo. In the adult, deregulation of vascular growth contributes to the pathology of devastating disorders including, but not restricted to cancer. We are interested in understanding the process by which blood vessels are formed and its regulation in vivo. Specifically, our research efforts focus on four major projects. a) Angiogenesis Inhibitors: Using a combination of transgenic mouse models, biochemistry and cell biology we investigate the biological function of thrombospondin-1, ADAMTS1 and ADAMTS8, all potential endogenous inhibitors of angiogenesis. Experiments are also designed to ascertain their potential threapeutic value in the suppression of tumor growth. b) Contribution of Notch1 to vascular morphogenesis: Recent linkage and knock-out studies have revealed that Notch signaling is required for morphogenesis and homeostasis of the vascular system. Using a Cre-lox strategy we are inactivating Notch1 from endothelial and smooth muscle cells. These experiments are complementary to expression arrays and several modalities of signaling studies in vitro. c) Regulation of vascular endothelial growth factor availability: We have recently found that specific proteases part take in the removal of VEGF from the matrix by cleaving the growth factor directly and releasing bioactive peptides. This release is dose-dependent and it is suppressed by specific inhibitors. Proteolytically processed VEGF is able to phosphorylate its receptors at the same molar ratio as the entire molecule. The biological significance of these findings is being studied with transgenic mice expressing different VEGF mutants. d) Role of progesterone receptor signaling in endothelial cells: Steroid hormones are essential for endometrial cycle, ovulation, implantation, and lactation. In addition they play significant roles in the cardiovascular system, yet relatively little is known about their effects on vascular cells. We are focusing on the role of progesterone receptor on endothelial cell biology using a combination of transgenic mouse models and signaling experiments in vitro.

Luisa Iruela-Arispe is a cell and developmental biologist and professor in the Department of Molecular, Cell and Developmental Biology. She earned her Ph.D. degree from the University of Sao Paulo in Brazil in 1989 and received post-doctoral training at the Universtity of Washington in Seattle. She was appointed Assistant Professor at the Department of Pathology at Harvard Medical School in 1994 where she stayed for four years. In 1998, Dr. Iruela-Arispe joined the faculty of the Department of Molecular, Cell and Developmental Biology at UCLA. Her research centers in understanding the molecular regulation of endothelial cell commitment and differentiation, patterning of the vasculature and homeostatic vascular function. This information has provided the basis to develop strategies to control vascular growth during disease, particularly in cancer. Dr. Iruela-Arispe is strongly committed to education. She is currently the Vice-Chair for teaching in her Department and provides instruction to both undergraduates and graduate students. She is actively involved in her field of research and has organized several meetings including two Gordon Conferences, one on Angiogenesis and a second on Vascular Biology. She is the founder and director of the Vascular Biology Training Program at UCLA.

Iruela-Arispe ML, Ortega MA and Vazquez F Anti-angiogenic domain of thrombospondin. Angiogenesis in Health and Disease: Basic Mechanisms and Clinical Applications 1999; .
Iruela-Arispe ML, Vazquez F and Ortega MA Anti-angiogenic domains shared by thrombospondins and ADAM/TS proteins. Anti-Cancer Proteins & Drugs: Structure, Function & Design 1999; 886: 58-66.
Iruela-Arispe ML, Rodriguez-Manzaneque JC, Abu-Jawdeh G Endometrial endothelial cells express estrogen and progesterone receptors and exhibit a tissue specific response to angiogenic growth factors. Microcirculation (New York, N.Y. : 1994) . 1999; 6(2): 127-40.
Dhanabal M, Ramchandran R, Volk R, Stillman IE, Lombardo M, Iruela-Arispe ML, Simons M and Sukhatme VP Endostatin: yeast production, mutants, and anti-tumor effect in renal cell carcinoma. Cancer Res 1999; 59: 189-197.
Wu H, Lee SH, Gao J, Liu X, Iruela-Arispe ML Inactivation of erythropoietin leads to defects in cardiac morphogenesis. Development (Cambridge, England) . 1999; 126(16): 3597-605.
Zhai Y, Yu J, Iruela-Arispe L, Huang WQ, Wang Z, Hayes AJ, Lu J, Jiang G, Rojas L, Lippman ME, Ni J, Yu GL, Li LY Inhibition of angiogenesis and breast cancer xenograft tumor growth by VEGI, a novel cytokine of the TNF superfamily. International journal of cancer. Journal international du cancer. . 1999; 82(1): 131-6.
Iruela-Arispe ML, Lombardo M, Krutzsch HC, Lawler J and Roberts DD Inhibition of angiogenesis by thrombospondin-1 is mediated by two independent regions within the type 1 repeats. Circulation 1999; 100: 1423-1431.
Vazquez F, Hastings G, Ortega MA, Lane TF, Oikemus S, Lombardo M and Iruela-Arispe ML METH1, a human ortholog of ADAMTS, and METH2 are members of a new family of proteins with angio-inhibitory activity. J. Biol. Chem 1999; 274: 23349-23357.
Vazquez F, Rodriguez-Manzaneque JC, Lydon JP, Edwards DP, OMalley BW and Iruela-Arispe ML Progesterone regulates proliferation of endothelial cells. J. Biol. Chem 1999; 274: 2185-2192.
Brown LF, Guidi AJ, Schnitt SJ, Van De Water L, Iruela-Arispe ML, Yeo TK, Tognazzi K, Dvorak HF Vascular stroma formation in carcinoma in situ, invasive carcinoma, and metastatic carcinoma of the breast. Clinical cancer research : an official journal of the American Association for Cancer Research. . 1999; 5(5): 1041-56.
Iruela-Arispe ML, Vazquez F, and Ortega MA Antiangiogenic domains shared by thrombospondins and metallospondins, a new family of angiogenic inhibitors. Ann. N.Y. Acad. Sci 2000; 886: 58-66.
Chandrasekaran L, He ZC, Al-Barazi H, Krutzch HC, Iruela-Arispe ML and Roberts DD Cell contact-dependent activation of a3?1 integrin modulates endothelial cell responses to thrombospondin-1. Mol. Biol. Cell 2000; 11: 2885-2900.
Rodriguez-Manzaneque JC, Milchanowski AB, Dufour EK, Leduc R and Iruela-Arispe ML Characterization of Meth-1/Adamts-1 Processing Reveals Two Distinct Active Forms. J. Biol. Chem 2000; 275: 33471-33479.
Carpizo D, Iruela-Arispe ML Endogenous regulators of angiogenesis--emphasis on proteins with thrombospondin--type I motifs. Cancer metastasis reviews. . 2000; 19(1-2): 159-65.
Rodriguez-Manzaneque JC, Graubert M and Iruela-Arispe ML Endothelial cell dysfunction following prolonged activation of progesterone receptor. Human Reproduction 2000; 15: 39-47.
Lee SW, Reimer CL, Fang L, Iruela-Arispe ML, Aaronson SA Overexpression of kinase-associated phosphatase (KAP) in breast and prostate cancer and inhibition of the transformed phenotype by antisense KAP expression. Molecular and cellular biology. . 2000; 20(5): 1723-32.
Pepper MS, Baetens D, Mandriota SJ, Di Sanza C, Oikemus S, Lane TF, Soriano JV, Montesano R, Iruela-Arispe ML Regulation of VEGF and VEGF receptor expression in the rodent mammary gland during pregnancy, lactation, and involution. Developmental dynamics : an official publication of the American Association of Anatomists. . 2000; 218(3): 507-24.
Pal S, Iruela-Arispe ML, Harvey VS, Zeng H, Nagy JA, Dvorak HF, Mukhopadhyay D Retinoic acid selectively inhibits the vascular permeabilizing effect of VPF/VEGF, an early step in the angiogenic cascade. Microvascular research. . 2000; 60(2): 112-20.
Streit M, Velasco P, Riccardi L, Spencer L, Brown LF, Janes L, Lange-Asschenfeldt B, Yano K, Hawighorst T, Iruela-Arispe L, Detmar M Thrombospondin-1 suppresses wound healing and granulation tissue formation in the skin of transgenic mice. The EMBO journal. . 2000; 19(13): 3272-82.
Dube A, Thai S, Gaspar J, Rudders S, Libermann TA, Iruela-Arispe L, Oettgen P Elf-1 is a transcriptional regulator of the Tie2 gene during vascular development. Circulation research. . 2001; 88(2): 237-44.
Saaristo A, Karkkainen MJ, Alitalo K, Montesano R, Iruela-Arispe ML, Pepper MS Growth factor regulation of physiological angiogenesis in the mammary gland. Vascular Morphogenesis in the Female Reproductive System 2001; Eds: Augustin, H.G., Iruela-Arispe, M.L., Rogers, : 25-40.
Rodriguez-Manzaneque JC, Lane TF, Ortega MA, Hynes RO, Lawler J and Iruela-Arispe ML Thrombospondin-1 suppresses tumor growth by a novel mechanism that includes blockade of matrix metalloproteinase-9 activation. PNAS. 2001; 22: 12485-12490.
Villa N, Walker L, Lindsell CE, Gasson J, Iruela-Arispe ML, Weinmaster G Vascular expression of Notch pathway receptors and ligands is restricted to arterial vessels. Mechanisms of development. . 2001; 108(1-2): 161-4.
Iruela-Arispe ML, Ortega MA, Oikemus S and Pepper MS Vascular morphogenesis in the mammary gland - introduction and overview. Vascular Morphogenesis in the Female Reproductive System 2001; Eds: Augustin, H.G., Iruela-Arispe, M.L., Rogers, : 3-24.
Graubert MD, Ortega MA, Kessel B, Mortola JF and Iruela-Arispe ML Vascular repair after menstruation involves regulation of vascular endothelial growthfactor-receptor phosphorylation by sFLT-1. Am J Pathol. 2001; 158(4): 1399-410.
Sandy JD, Westling J, Kenagy RD, Iruela-Arispe ML, Verscharen C, Rodriguez-Mazaneque JC, Zimmermann DR, Lemire JM, Fischer JW, Wight TN, Clowes AW Versican V1 proteolysis in human aorta in vivo occurs at the Glu441-Ala442 bond, a site that is cleaved by recombinant ADAMTS-1 and ADAMTS-4. The Journal of biological chemistry. . 2001; 276(16): 13372-8.
Park Ho-Jin, Kong Dequan, Iruela-Arispe Luisa, Begley Ulrike, Tang Dongjiang, Galper Jonas B 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors interfere with angiogenesis by inhibiting the geranylgeranylation of RhoA. Circulation research. 2002; 91(2): 143-50.
Rodríguez-Manzaneque Juan Carlos, Westling Jennifer, Thai Shelley N-M, Luque Alfonso, Knauper Vera, Murphy Gillian, Sandy John D, Iruela-Arispe M Luisa ADAMTS1 cleaves aggrecan at multiple sites and is differentially inhibited by metalloproteinase inhibitors. Biochemical and biophysical research communications. 2002; 293(1): 501-8.
Murayama Yuichi, Viñuela Fernando, Tateshima Satoshi, Gonzalez Nestor R, Song Joon K, Mahdavieh Haleh, Iruela-Arispe Luisa Cellular responses of bioabsorbable polymeric material and Guglielmi detachable coil in experimental aneurysms. Stroke; a journal of cerebral circulation. 2002; 33(4): 1120-8.
Thai Shelley N-M, Iruela-Arispe M Luisa Expression of ADAMTS1 during murine development. Mechanisms of development. 2002; 115(1-2): 181-5.
Gaspar John, Thai Shelley, Voland Carole, Dube Antoinise, Libermann Towia A, Iruela-Arispe M Luisa, Oettgen Peter Opposing functions of the Ets factors NERF and ELF-1 during chicken blood vessel development. Arteriosclerosis, thrombosis, and vascular biology. 2002; 22(7): 1106-12.
Adams Jason Y, Johnson Mai, Sato Makoto, Berger Frank, Gambhir Sanjiv S, Carey Michael, Iruela-Arispe M Luisa, Wu Lily Visualization of advanced human prostate cancer lesions in living mice by a targeted gene transfer vector and optical imaging. Nature medicine. 2002; 8(8): 891-7.
Luque Alfonso, Carpizo Darren R, Iruela-Arispe M Luisa ADAMTS1/METH1 inhibits endothelial cell proliferation by direct binding and sequestration of VEGF165. The Journal of biological chemistry. 2003; 278(26): 23656-65.
Iruela-Arispe M Luisa, Carpizo Darren, Luque Alfonso ADAMTS1: a matrix metalloprotease with angioinhibitory properties. Annals of the New York Academy of Sciences. 2003; 995(26): 183-90.
Ellwood-Yen Katharine, Graeber Thomas G, Wongvipat John, Iruela-Arispe M Luisa, Zhang JianFeng, Matusik Robert, Thomas George V, Sawyers Charles L Myc-driven murine prostate cancer shares molecular features with human prostate tumors. Cancer cell. 2003; 4(3): 223-38.
Jilani Shahla M, Murphy Thomas J, Thai Shelley N M, Eichmann Anne, Alva Jackelyn A, Iruela-Arispe M Luisa Selective binding of lectins to embryonic chicken vasculature. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 2003; 51(5): 597-604.
Calzada Maria J, Zhou Longen, Sipes John M, Zhang Jane, Krutzsch Henry C, Iruela-Arispe M Luisa, Annis Douglas S, Mosher Deane F, Roberts David D Alpha4beta1 integrin mediates selective endothelial cell responses to thrombospondins 1 and 2 in vitro and modulates angiogenesis in vivo. Circulation research. 2004; 94(4): 462-70.
Iruela-Arispe Luisa Angiogenesis: novel and basic science insights and human therapy - keystone symposium. IDrugs : the investigational drugs journal. 2004; 7(2): 111-3.
Fiala Milan, Murphy Thomas, MacDougall James, Yang Wendy, Luque Alfonso, Iruela-Arispe Luisa, Cashman John, Buga Georgette, Byrns Russel E, Barbaro Giuseppe, Arthos James HAART drugs induce mitochondrial damage and intercellular gaps and gp120 causes apoptosis. Cardiovascular toxicology. 2004; 4(4): 327-37.
Alva Jackelyn A, Iruela-Arispe M Luisa Notch signaling in vascular morphogenesis. Current opinion in hematology. 2004; 11(4): 278-83.
Iruela-Arispe M Luisa, Luque Alfonso, Lee Nathan Thrombospondin modules and angiogenesis. The international journal of biochemistry & cell biology. 2004; 36(6): 1070-8.
Thomas-Tikhonenko Andrei, Iruela-Arispe M Luisa Whence thrombospondin?. Cancer biology & therapy. 2004; 3(4): 406-7.
Krampert Monika, Kuenzle Sandra, Thai Shelley N-M, Lee Nathan, Iruela-Arispe M Luisa, Werner Sabine ADAMTS1 proteinase is up-regulated in wounded skin and regulates migration of fibroblasts and endothelial cells. The Journal of biological chemistry. 2005; 280(25): 23844-52.
Cho Hyun-Mi, Rosenblatt Joseph D, Kang Young-Sook, Iruela-Arispe M Luisa, Morrison Sherie L, Penichet Manuel L, Kwon Young-Guen, Kim Tae-Woong, Webster Keith A, Nechustan Hovav, Shin Seung-Uon Enhanced inhibition of murine tumor and human breast tumor xenografts using targeted delivery of an antibody-endostatin fusion protein. Molecular cancer therapeutics. 2005; 4(6): 956-67.
Lee Nathan V, Rodriguez-Manzaneque Juan Carlos, Thai Shelley N-M, Twal Waleed O, Luque Alfonso, Lyons Karen M, Argraves W Scott, Iruela-Arispe M Luisa Fibulin-1 acts as a cofactor for the matrix metalloprotease ADAMTS-1. The Journal of biological chemistry. 2005; 280(41): 34796-804.
Lee Sunyoung, Jilani Shahla M, Nikolova Ganka V, Carpizo Darren, Iruela-Arispe M Luisa Processing of VEGF-A by matrix metalloproteinases regulates bioavailability and vascular patterning in tumors. The Journal of cell biology. 2005; 169(4): 681-91.
Iruela-Arispe ML Vascular Development and Angiogenesis. Encyclopedia of Molecular Cell Biology and Molecular Medicine 2005; .
Torres-Collado, A.X., Kisiel, W., Iruela-Arispe, M.L., Rodriguez-Manzaneque, J.C. . ADAMTS1 interacts with, cleaves and modifies the extracellular location of the matrix inhibitor tissue factor pathway inhibitor-2 (TFPI-2). J Biol Chem 2006; 281: 17827-17837.
Lee Nathan V, Sato Makoto, Annis Douglas S, Loo Joseph A, Wu Lily, Mosher Deane F, Iruela-Arispe M Luisa ADAMTS1 mediates the release of antiangiogenic polypeptides from TSP1 and 2. The EMBO journal. 2006; 25(22): 5270-83.
Hashimoto-Partyka Minako K, Lydon John P, Iruela-Arispe M Luisa Generation of a mouse for conditional excision of progesterone receptor. Genesis (New York, N.Y. : 2000). 2006; 44(8): 391-5.
Zovein Ann C, Iruela-Arispe M Luisa My O'Myeloid, a tale of two lineages. Proceedings of the National Academy of Sciences of the United States of America. 2006; 103(35): 12959-60.
Kern Christine B, Twal Waleed O, Mjaatvedt Corey H, Fairey Sarah E, Toole Bryan P, Iruela-Arispe M Luisa, Argraves W Scott Proteolytic cleavage of versican during cardiac cushion morphogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 2006; 235(8): 2238-47.
Barber, C.L. and Iruela-Arispe, M.L. The Ever-Elusive Endothelial Progenitor Cell: Identities, Functions and Clinical Implications. . Pediatric Res 2006; 59: 26R-32R.
Barber Chad L, Iruela-Arispe M Luisa The ever-elusive endothelial progenitor cell: identities, functions and clinical implications. Pediatric research. 2006; 59(4 Pt 2): 26R-32R.
Alva Jackelyn A, Zovein Ann C, Monvoisin Arnaud, Murphy Thomas, Salazar Anthony, Harvey Natasha L, Carmeliet Peter, Iruela-Arispe M Luisa VE-Cadherin-Cre-recombinase transgenic mouse: a tool for lineage analysis and gene deletion in endothelial cells. Developmental dynamics : an official publication of the American Association of Anatomists. 2006; 235(3): 759-67.
Monvoisin, A., Alva, J.A., Hofmann, J.J., Zovein, A.C., Lane, T.F., and Iruela-Arispe, M.L. VE-cadherin-CreER(T2) transgenic mice: A model for inducible recombination in the endothelium. Dev Dyn 2006; 235: 3413-3422.
Monvoisin Arnaud, Alva Jackelyn A, Hofmann Jennifer J, Zovein Ann C, Lane Timothy F, Iruela-Arispe M Luisa VE-cadherin-CreERT2 transgenic mouse: a model for inducible recombination in the endothelium. Developmental dynamics : an official publication of the American Association of Anatomists. 2006; 235(12): 3413-22.
Iruela-Arispe M Luisa When it comes to blocking lymphatics, it is all a question of time. The American journal of pathology. 2006; 169(2): 347-50.
Lee Sunyoung, Chen Tom T, Barber Chad L, Jordan Maria C, Murdock Jared, Desai Sharina, Ferrara Napoleone, Nagy Andras, Roos Kenneth P, Iruela-Arispe M Luisa Autocrine VEGF signaling is required for vascular homeostasis. Cell. 2007; 130(4): 691-703.
Hellström Mats, Phng Li-Kun, Hofmann Jennifer J, Wallgard Elisabet, Coultas Leigh, Lindblom Per, Alva Jackelyn, Nilsson Ann-Katrin, Karlsson Linda, Gaiano Nicholas, Yoon Keejung, Rossant Janet, Iruela-Arispe M Luisa, Kalén Mattias, Gerhardt Holger, Betsholtz Christer Dll4 signalling through Notch1 regulates formation of tip cells during angiogenesis. Nature. 2007; 445(7129): 776-80.
Hellstrom, M., Phng, L.K., Hofmann, J.J., Wallgard, El., Coultas, L., Limdblom, P., Alva, J., Nilsson, L., Gaiano, N., Yoon, K., Rossant, J., Iruela-Arispe, M.L., Kalen, M., Gerhardt, H., and Betsholtz, C. Dll4/Notch1 signaling is required for selection of single endothelial tip cells. . Nature 2007; 445: 776-780.
Lundkvist Andrea, Lee Sunyoung, Iruela-Arispe Luisa, Betsholtz Christer, Gerhardt Holger Growth factor gradients in vascular patterning. Novartis Foundation symposium. 2007; 283(7129): 194-201; discussion 201-6, 238-41.
Lee Min, Chen Tom T, Iruela-Arispe M Luisa, Wu Benjamin M, Dunn James C Y Modulation of protein delivery from modular polymer scaffolds. Biomaterials. 2007; 28(10): 1862-70.
Hofmann Jennifer J, Luisa Iruela-Arispe M Notch expression patterns in the retina: An eye on receptor-ligand distribution during angiogenesis. Gene expression patterns : GEP. 2007; 7(4): 461-70.
Hofmann Jennifer J, Iruela-Arispe M Luisa Notch signaling in blood vessels: who is talking to whom about what?. Circulation research. 2007; 100(11): 1556-68.
Lee, S. and Iruela-Arispe, M.L. The Extracellular Matrix and VEGF Processing. . Anti-Angiogenesis, 2nd Edition 2007; .
Lee, N.V. and Iruela-Arispe, M.L. Thrombospondins and Angiogenesis. . Tumor Angiogenesis: Basic Mechanisms and Cancer Therapy. 2007; 233-245.
Yang Li V, Radu Caius G, Roy Meenakshi, Lee Sunyoung, McLaughlin Jami, Teitell Michael A, Iruela-Arispe M Luisa, Witte Owen N Vascular abnormalities in mice deficient for the G protein-coupled receptor GPR4 that functions as a pH sensor. Molecular and cellular biology. 2007; 27(4): 1334-47.
Suárez Yajaira, Fernández-Hernando Carlos, Yu Jun, Gerber Scott A, Harrison Kenneth D, Pober Jordan S, Iruela-Arispe M Luisa, Merkenschlager Matthias, Sessa William C Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis. Proceedings of the National Academy of Sciences of the United States of America. 2008; 105(37): 14082-7.
Stankunas Kryn, Hang Calvin T, Tsun Zhi-Yang, Chen Hanying, Lee Nathan V, Wu Jiang I, Shang Ching, Bayle J Henri, Shou Weinian, Iruela-Arispe M Luisa, Chang Ching-Pin Endocardial Brg1 represses ADAMTS1 to maintain the microenvironment for myocardial morphogenesis. Developmental cell. 2008; 14(2): 298-311.
Iruela-Arispe, M.L. Endothelial Cell Activation. Angiogenesis: An Integrative Approach from Science to Medicine 2008; 35-43.
Zovein Ann C, Hofmann Jennifer J, Lynch Maureen, French Wendy J, Turlo Kirsten A, Yang Yanan, Becker Michael S, Zanetta Lucia, Dejana Elisabetta, Gasson Judith C, Tallquist Michelle D, Iruela-Arispe M Luisa Fate tracing reveals the endothelial origin of hematopoietic stem cells. Cell stem cell. 2008; 3(6): 625-36.
Misra Sanjay, Lee Nathan, Fu Alex A, Raghavakaimal Sreekumar, Mandrekar Jaywant, Bjarnason Haraldur, McKusick Michael A, Iruela-Arispe Luisa, Mukhopadhyay Debabrata Increased expression of a disintegrin and metalloproteinase thrombospondin 1 in thrombosed hemodialysis grafts. Journal of vascular and interventional radiology : JVIR. 2008; 19(1): 111-9.
Guo Wei, Lasky Joseph L, Chang Chun-Ju, Mosessian Sherly, Lewis Xiaoman, Xiao Yun, Yeh Jennifer E, Chen James Y, Iruela-Arispe M Luisa, Varella-Garcia Marileila, Wu Hong Multi-genetic events collaboratively contribute to Pten-null leukaemia stem-cell formation. Nature. 2008; 453(7194): 529-33.
Guo, W., Lasky, J.L., Chang, C.J., Mosessian, S., Lewis, X., Xiao, Y., Yeh, J.E., Chen, J.Y., Iruela-Arispe, M.L., Varella-Garcia, M., and Wu, H. Multiple genetic and molecular alterations cooperatively contribute LSC formation in a Pten-deficient leukemia model. . Nature 2008; 453: 529-533.
Iruela-Arispe M Luisa Regulation of thrombospondin1 by extracellular proteases. Current drug targets. 2008; 9(10): 863-8.
Iruela-Arispe M Luisa, Davis George E Cellular and molecular mechanisms of vascular lumen formation. Developmental cell. 2009; 16(2): 222-31.
Rodríguez-Manzaneque Juan Carlos, Carpizo Darren, Plaza-Calonge María del Carmen, Torres-Collado Antoni Xavier, Thai Shelley N-M, Simons Michael, Horowitz Arie, Iruela-Arispe M Luisa Cleavage of syndecan-4 by ADAMTS1 provokes defects in adhesion. The international journal of biochemistry & cell biology. 2009; 41(4): 800-10.
Speck, N.A. and Iruela-Arispe, M.L. Conditional Cre/LoxP Strategies for the Study of Hematopoietic Stem Cell Formation. Blood Cells Mol. Dis 2009; 43: 6-11.
Speck Nancy A, Iruela-Arispe M Luisa Conditional Cre/LoxP strategies for the study of hematopoietic stem cell formation. Blood cells, molecules & diseases. 2009; 43(1): 6-11.
Skuli Nicolas, Liu Liping, Runge Anja, Wang Tao, Yuan Lijun, Patel Sunny, Iruela-Arispe Luisa, Simon M Celeste, Keith Brian Endothelial deletion of hypoxia-inducible factor-2alpha (HIF-2alpha) alters vascular function and tumor angiogenesis. Blood. 2009; 114(2): 469-77.
Anderson Sean M, Chen Tom T, Iruela-Arispe M Luisa, Segura Tatiana The phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) by engineered surfaces with electrostatically or covalently immobilized VEGF. Biomaterials. 2009; 30(27): 4618-28.
Zovein Ann C, Iruela-Arispe M Luisa Time to cut the cord: placental HSCs grow up. Cell stem cell. 2009; 5(4): 351-2.
Chen, T.T., Luque, A., Lee, S., Anderson, S.M., Segura, T., Iruela-Arispe, M.L. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. . J Cell Biol 2010; 188(4): 595-609.
Chen Tom T, Luque Alfonso, Lee Sunyoung, Anderson Sean M, Segura Tatiana, Iruela-Arispe M Luisa Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. The Journal of cell biology. 2010; 188(4): 595-609.
Zovein Ann C, Luque Alfonso, Turlo Kirsten A, Hofmann Jennifer J, Yee Kathleen M, Becker Michael S, Fassler Reinhard, Mellman Ira, Lane Timothy F, Iruela-Arispe M Luisa Beta1 integrin establishes endothelial cell polarity and arteriolar lumen formation via a Par3-dependent mechanism. Developmental cell. 2010; 18(1): 39-51.
da Silva Rita Graça, Tavora Bernardo, Robinson Stephen D, Reynolds Louise E, Szekeres Charles, Lamar John, Batista Sílvia, Kostourou Vassiliki, Germain Mitchel A, Reynolds Andrew R, Jones Dylan T, Watson Alan R, Jones Janet L, Harris Adrian, Hart Ian R, Iruela-Arispe M Luisa, Dipersio C Michael, Kreidberg Jordan A, Hodivala-Dilke Kairbaan M Endothelial alpha3beta1-integrin represses pathological angiogenesis and sustains endothelial-VEGF. The American journal of pathology. 2010; 177(3): 1534-48.
Arroyo Alicia G, Iruela-Arispe M Luisa Extracellular matrix, inflammation, and the angiogenic response. Cardiovascular research. 2010; 86(2): 226-35.
Hofmann Jennifer J, Zovein Ann C, Koh Huilin, Radtke Freddy, Weinmaster Gerry, Iruela-Arispe M Luisa Jagged1 in the portal vein mesenchyme regulates intrahepatic bile duct development: insights into Alagille syndrome. Development (Cambridge, England). 2010; 137(23): 4061-72.
Stockmann Christian, Kerdiles Yann, Nomaksteinsky Marc, Weidemann Alexander, Takeda Norihiko, Doedens Andrew, Torres-Collado Antonio X, Iruela-Arispe Luisa, Nizet Victor, Johnson Randall S Loss of myeloid cell-derived vascular endothelial growth factor accelerates fibrosis. Proceedings of the National Academy of Sciences of the United States of America. 2010; 107(9): 4329-34.
Warren Carmen M, Iruela-Arispe M Luisa Signaling circuitry in vascular morphogenesis. Current opinion in hematology. 2010; 17(3): 213-8.
Priceman Saul J, Sung James L, Shaposhnik Zory, Burton Jeremy B, Torres-Collado Antoni X, Moughon Diana L, Johnson Mai, Lusis Aldons J, Cohen Donald A, Iruela-Arispe M Luisa, Wu Lily Targeting distinct tumor-infiltrating myeloid cells by inhibiting CSF-1 receptor: combating tumor evasion of antiangiogenic therapy. Blood. 2010; 115(7): 1461-71.
Corada Monica, Nyqvist Daniel, Orsenigo Fabrizio, Caprini Andrea, Giampietro Costanza, Taketo Makoto M, Iruela-Arispe M Luisa, Adams Ralf H, Dejana Elisabetta The Wnt/beta-catenin pathway modulates vascular remodeling and specification by upregulating Dll4/Notch signaling. Developmental cell. 2010; 18(6): 938-49.
Lee Yoon-Jin, Koch Moritz, Karl Daniel, Torres-Collado Antoni X, Fernando Namali T, Rothrock Courtney, Kuruppu Darshini, Ryeom Sandra, Iruela-Arispe M Luisa, Yoon Sam S Variable inhibition of thrombospondin 1 against liver and lung metastases through differential activation of metalloproteinase ADAMTS1. Cancer research. 2010; 70(3): 948-56.
Zovein Ann C, Turlo Kirsten A, Ponec Ryan M, Lynch Maureen R, Chen Kevin C, Hofmann Jennifer J, Cox Timothy C, Gasson Judith C, Iruela-Arispe M Luisa Vascular remodeling of the vitelline artery initiates extravascular emergence of hematopoietic clusters. Blood. 2010; 116(18): 3435-44.
Turlo Kirsten A, Gallaher Sean D, Vora Roshni, Laski Frank A, Iruela-Arispe M Luisa When Cre-mediated recombination in mice does not result in protein loss. Genetics. 2010; 186(3): 959-67.
Iruela-Arispe M Luisa LUMENating blood vessels. Developmental cell. 2011; 20(4): 412-4.
Ziyad Safiyyah, Iruela-Arispe M Luisa Molecular mechanisms of tumor angiogenesis. Genes & cancer. 2011; 2(12): 1085-96.
Anderson Sean M, Shergill Bhupinder, Barry Zachary T, Manousiouthakis Eleana, Chen Tom T, Botvinick Elliot, Platt Manu O, Iruela-Arispe M Luisa, Segura Tatiana VEGF internalization is not required for VEGFR-2 phosphorylation in bioengineered surfaces with covalently linked VEGF. Integrative biology : quantitative biosciences from nano to macro. 2011; 3(9): 887-96.
Turlo Kirsten A, Noel Onika D V, Vora Roshni, LaRussa Marie, Fassler Reinhard, Hall-Glenn Faith, Iruela-Arispe M Luisa An essential requirement for β1 integrin in the assembly of extracellular matrix proteins within the vascular wall. Developmental biology. 2012; 365(1): 23-35.
Avraham-Davidi Inbal, Ely Yona, Pham Van N, Castranova Daniel, Grunspan Moshe, Malkinson Guy, Gibbs-Bar Liron, Mayseless Oded, Allmog Gabriella, Lo Brigid, Warren Carmen M, Chen Tom T, Ungos Josette, Kidd Kameha, Shaw Kenna, Rogachev Ilana, Wan Wuzhou, Murphy Philip M, Farber Steven A, Carmel Liran, Shelness Gregory S, Iruela-Arispe M Luisa, Weinstein Brant M, Yaniv Karina ApoB-containing lipoproteins regulate angiogenesis by modulating expression of VEGF receptor 1. Nature medicine. 2012; 18(6): 967-73.
Hall-Glenn Faith, De Young R Andrea, Huang Bau-Lin, van Handel Ben, Hofmann Jennifer J, Chen Tom T, Choi Aaron, Ong Jessica R, Benya Paul D, Mikkola Hanna, Iruela-Arispe M Luisa, Lyons Karen M CCN2/connective tissue growth factor is essential for pericyte adhesion and endothelial basement membrane formation during angiogenesis. PloS one. 2012; 7(2): e30562.
He Huanhuan, Xu Jingying, Warren Carmen M, Duan Dan, Li Xinmin, Wu Lily, Iruela-Arispe M Luisa Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages. Blood. 2012; 120(15): 3152-62.
Hofmann Jennifer J, Briot Anais, Enciso Josephine, Zovein Ann C, Ren Shuxun, Zhang Zhen W, Radtke Freddy, Simons Michael, Wang Yibin, Iruela-Arispe M Luisa Endothelial deletion of murine Jag1 leads to valve calcification and congenital heart defects associated with Alagille syndrome. Development (Cambridge, England). 2012; 139(23): 4449-60.
Cooley Marion A, Fresco Victor M, Dorlon Margaret E, Twal Waleed O, Lee Nathan V, Barth Jeremy L, Kern Christine B, Iruela-Arispe M Luisa, Argraves W Scott Fibulin-1 is required during cardiac ventricular morphogenesis for versican cleavage, suppression of ErbB2 and Erk1/2 activation, and to attenuate trabecular cardiomyocyte proliferation. Developmental dynamics : an official publication of the American Association of Anatomists. 2012; 241(2): 303-14.
Scheppke Lea, Murphy Eric A, Zarpellon Alessandro, Hofmann Jennifer J, Merkulova Alona, Shields David J, Weis Sara M, Byzova Tatiana V, Ruggeri Zaverio M, Iruela-Arispe M Luisa, Cheresh David A Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane. Blood. 2012; 119(9): 2149-58.
Domigan Courtney K, Iruela-Arispe M Luisa Recent advances in vascular development. Current opinion in hematology. 2012; 19(3): 176-83.
Howell Matthew D, Torres-Collado Antoni X, Iruela-Arispe M Luisa, Gottschall Paul E Selective decline of synaptic protein levels in the frontal cortex of female mice deficient in the extracellular metalloproteinase ADAMTS1. PloS one. 2012; 7(10): e47226.
Chhabra Akanksha, Lechner Andrew J, Ueno Masaya, Acharya Asha, Van Handel Ben, Wang Yanling, Iruela-Arispe M Luisa, Tallquist Michelle D, Mikkola Hanna K A Trophoblasts regulate the placental hematopoietic niche through PDGF-B signaling. Developmental cell. 2012; 22(3): 651-9.
Goddard Lauren M, Iruela-Arispe M Luisa Cellular and molecular regulation of vascular permeability. Thrombosis and haemostasis. 2013; 109(3): 407-15.
Cristofaro Brunella, Shi Yu, Faria Marcella, Suchting Steven, Leroyer Aurelie S, Trindade Alexandre, Duarte Antonio, Zovein Ann C, Iruela-Arispe M Luisa, Nih Lina R, Kubis Nathalie, Henrion Daniel, Loufrani Laurent, Todiras Mihail, Schleifenbaum Johanna, Gollasch Maik, Zhuang Zhen W, Simons Michael, Eichmann Anne, le Noble Ferdinand Dll4-Notch signaling determines the formation of native arterial collateral networks and arterial function in mouse ischemia models. Development (Cambridge, England). 2013; 140(8): 1720-9.
Roodhart Jeanine M L, He Huanhuan, Daenen Laura G M, Monvoisin Arnaud, Barber Chad, Amersfoort Miranda van, Hofmann Jennifer J, Radtke Freddy, Lane Timothy F, Voest Emile E, Iruela-Arispe M Luisa Notch1 regulates angio-supportive bone marrow-derived cells: relevance to chemoresistance. Blood. 2013; .
Janzen Deanna M, Rosales Miguel A, Paik Daniel Y, Lee Daniel S, Smith Daniel A, Witte Owen N, Iruela-Arispe M Luisa, Memarzadeh Sanaz Progesterone receptor signaling in the microenvironment of endometrial cancer influences its response to hormonal therapy. Cancer research. 2013; .
Mack Julia J, Youssef Khalid, Noel Onika D V, Lake Michael P, Wu Ashley, Iruela-Arispe M Luisa, Bouchard Louis-S Real-time maps of fluid flow fields in porous biomaterials. Biomaterials. 2013; 34(8): 1980-6.
Baer Caroline, Squadrito Mario Leonardo, Iruela-Arispe M Luisa, De Palma Michele Reciprocal interactions between endothelial cells and macrophages in angiogenic vascular niches. Experimental cell research. 2013; 140(8): .
Goddard Lauren M, Ton Amy N, Org Tõnis, Mikkola Hanna K A, Iruela-Arispe M Luisa Selective suppression of endothelial cytokine production by progesterone receptor. Vascular pharmacology. 2013; .
Corada, M., Orsenigo, F., Morini, M.F., Pitulescu, M. Bhat, G., Nyqvist, D., Breviario, F., Conti, V., Briot, A., Iruela-Arispe, M.L., Adams, R.H., Dejana, E. Sox17 is indispensable for acquisition and maintenance of arterial identity. Nature Commun 2013; 4: .
Defalco Tony, Saraswathula Anirudh, Briot Anais, Iruela-Arispe M Luisa, Capel Blanche Testosterone levels influence mouse fetal leydig cell progenitors through notch signaling. Biology of reproduction. 2013; 88(4): 91.
Milde, F., Iruela-Arispe, M.L., Koumoutsakos, P. The Mouse Retina in 3D: Quantification of vascular growth and remodeling. Integrative Biol 2013; 5(12): 1426-1438.
Iruela-Arispe M Luisa, Beitel Greg J Tubulogenesis. Development (Cambridge, England). 2013; 140(14): 2851-5.
Turlo Kirsten A, Scapa Jason, Bagher Pooneh, Jones Allan W, Feil Robert, Korthuis Ronald J, Segal Steven S, Iruela-Arispe M Luisa β1-integrin is essential for vasoregulation and smooth muscle survival in vivo. Arteriosclerosis, thrombosis, and vascular biology. 2013; 33(10): 2325-35.
Warren, C.M., Ziyad, S., Briot, A., Der, A., Iruela-Arispe, M.L. A Ligand-Independent VEGFR2 Signaling Pathway Limits Angiogenic Responses in Diabetes. Science Signaling 2014; 7(307): .
Griffin, D.R., Borrajo, J., Soon, A., Acosta-Velez, G.F., Oshita, V., Darling, N., Mack, J., Barker, T., Iruela-Arispe, M.L., Segura, T. Hybrid Photopatterned Enzymatic Reaction (HyPER) for in Situ Cell Manipulation. Chemobiochem 2014; 15(2): 233-242.
Michael Iacovos P, Westenskow Peter D, Hacibekiroglu Sabiha, Greenwald Alissa Cohen, Ballios Brian G, Kurihara Toshihide, Li Zhijie, Warren Carmen M, Zhang Puzheng, Aguilar Edith, Donaldson Laura, Marchetti Valentina, Baba Takeshi, Hussein Samer M, Sung Hoon-Ki, Iruela-Arispe M Luisa, Rini James M, van der Kooy Derek, Friedlander Martin, Nagy Andras Local acting Sticky-trap inhibits vascular endothelial growth factor dependent pathological angiogenesis in the eye. EMBO molecular medicine. 2014; 6(5): 604-23.
Warren Carmen M, Iruela-Arispe M Luisa Podosome rosettes precede vascular sprouts in tumour angiogenesis. Nature cell biology. 2014; 16(10): 928-30.
Goddard, L.M., Murphy, J.T., Org, T., Enciso, J.M., Hashimoto-Partyka, M.K., Warren, C.M., Domigan, C.K., McDonald, A.I., He, H., Sanchez, L.A., Allen, N.C., Orsenigo, F., Chao, L.C., Dejana, E., Tontonoz, P., Mikkola, H.K.A., Iruela-Arispe, M.L. Progesterone receptor in the vascular endothelium triggers physiological uterine permeability pre-implantation. Cell 2014; 156(3): 549-562.
Briot Anais, Jaroszewicz Artur, Warren Carmen M, Lu Jing, Touma Marlin, Rudat Carsten, Hofmann Jennifer J, Airik Rannar, Weinmaster Gerry, Lyons Karen, Wang Yibin, Kispert Andreas, Pellegrini Matteo, Iruela-Arispe M Luisa Repression of Sox9 by Jag1 is continuously required to suppress the default chondrogenic fate of vascular smooth muscle cells. Developmental cell. 2014; 31(6): 707-21.
Domigan Courtney K, Iruela-Arispe M Luisa Stealing VEGF from thy neighbor. Cell. 2014; 159(3): 473-4.
Nowak-Sliwinska Patrycja, Segura Tatiana, Iruela-Arispe M Luisa The chicken chorioallantoic membrane model in biology, medicine and bioengineering. Angiogenesis. 2014; 17(4): 779-804.
Rodri­guez-Manzaneque Juan Carlos, Fernandez-Rodriguez R., Rodriguez-Baena Francisco Javier, Iruela-Arispe M Luisa ADAMTS proteases in vascular biology. Matrix biology : journal of the International Society for Matrix Biology. 2015; 44-46(11): 38-45.
Domigan Courtney K, Warren Carmen M, Antanesian Vaspour, Happel Katharina, Ziyad Safiyyah, Lee Sunyoung, Krall Abigail, Duan Lewei, Torres-Collado Antoni X, Castellani Lawrence W, Elashoff David, Christofk Heather R, van der Bliek Alexander M, Potente Michael, Iruela-Arispe M Luisa Autocrine VEGF maintains endothelial survival through regulation of metabolism and autophagy. Journal of cell science. 2015; 128(12): 2236-48.
Briot Anais, Iruela-Arispe M Luisa Blockade of specific NOTCH ligands: a new promising approach in cancer therapy. Cancer discovery. 2015; 5(2): 112-4.
Domigan Courtney K, Ziyad Safiyyah, Iruela-Arispe M Luisa Canonical and noncanonical vascular endothelial growth factor pathways: new developments in biology and signal transduction. Arteriosclerosis, thrombosis, and vascular biology. 2015; 35(1): 30-9.
Hernandez-Garci­a Ricardo, Iruela-Arispe M Luisa, Reyes-Cruz Guadalupe, Vazquez-Prado Jose© Endothelial RhoGEFs: A systematic analysis of their expression profiles in VEGF-stimulated and tumor endothelial cells. Vascular pharmacology. 2015; .
McDonald Austin I, Iruela-Arispe M Luisa Healing arterial ulcers: Endothelial lining regeneration upon vascular denudation injury. Vascular pharmacology. 2015; 72: 9-15.
Moughon Diana, He Huanhuan, Schokrpur Shiruyeh, Jiang Ziyue Karen, Yaqoob Madeeha, David John, Lin Crystal, Iruela-Arispe M Luisa, Dorigo Oliver, Wu Lily Macrophage Blockade Using CSF1R Inhibitors Reverses The Vascular Leakage Underlying Malignant Ascites In Late-Stage Epithelial Ovarian Cancer. Cancer research. 2015; .
Simons Michael, Alitalo Kari, Annex Brian H, Augustin Hellmut G, Beam Craig, Berk Bradford C, Byzova Tatiana, Carmeliet Peter, Chilian William, Cooke John P, Davis George E, Eichmann Anne, Iruela-Arispe M Luisa, Keshet Eli, Sinusas Albert J, Ruhrberg Christiana, Woo Y Joseph, Dimmeler Stefanie, Dimmeler Stefanie State-of-the-Art Methods for Evaluation of Angiogenesis and Tissue Vascularization: A Scientific Statement From the American Heart Association. Circulation research. 2015; 116(11): e99-132.
Domigan CK, Warren CM, Antanesian V, Happel K, Ziyad S, Lee S, Krall A, Duan L, Torres-Collado AX, Castellani LW, Elashoff D, Christofk HR, van der Bliek AM, Potente M, Iruela-Arispe ML. Autocrine VEGF maintains endothelial survival through regulation of metabolism and autophagy. J Cell Sci 2015; 128(12): 2236-48.
McDonald, A., Iruela-Arispe, M.L. Healing arterial ulcers: Endothelial lining regeneration upon vascular denudation injury. Vascul Pharmacol 2015; 72: 9-15.
Moughon, D.L., He, H., Schokrpur, S., Jiang, Z.K., Yaqoob, M., David, J., Lin, C., Iruela-Arispe, M.L., Dorigo, O., Wu, L. Macrophage blockade using CSF1R inhibitors reverses the vascular leakage underlying malignant ascites in late-stage epithelial ovarian cancer. Cancer Res 2015; 75(22): 4742-52.
Hernandez-Garcia, R., Iruela-Arispe, M.L., Reyes-Cruz, G., Vazquez-Prado, J. Endothelial RhoGEFs: A systematic analysis of their expression profiles in VEGF-stimulated and tumor endothelial cells. Vascul Pharmacol 2015; 74: 60-72.
Rannou, E., Francois, A., Toullec, A., Guipaud, O., Buard, V., Tarlet, G., Mintet, E., Jaillet, C., Iruela-Arispe, M.L., Benderitter, M., Sabourin, J.C., Milliat, F. In vivo evidence for an endothelium-dependent mechanism in radiation-induced normal tissue injury. Sci Rep 2015; 5: 15738.
Briot, A., Civelek, M., Seki, A., Hoi, K., Mack, J.J., Lee, S.D., Kim, J., Hong, C., Yu, J., Fishbein, G.A., Vakili, L., Fogelman, A.M., Fishbein M.C., Lusis, A.J., Tontonoz, P., Navab, M., Berliner, J.A., Iruela-Arispe, M.L. Endothelial NOTCH1 is suppressed by circulating lipids and antagonizes inflammation during atherosclerosis. J Exp Med 2015; 212(12): 2147-63.
Youssef, K., Jarenwattananon, N., Archer, B., Mack, J., Iruela-Arispe, L., Bouchard, L. R-D flow control in porous scaffolds: Toward a next generation of bioreactors. IEEE Trans Biomed Eng 2016; .
Uebelhoer, M., Iruela-Arispe, M.L. Cross-talk between signaling and metabolism in the vasculature. . Vascular Pharm 2016; 83: 4-9.
Briot, A., Bouloumie, A., Iruela-Arispe, M.L. Notch, lipids, and endothelial cells. Current Opin Lipidol 2016; 27(5): 513-20.
He, H., Mack, J.J., Guc E., Warren, C.M., Squadrito, M.L., Kilarski, W.M., Baer, C., Freshman, R.D., McDonald, A.I., Ziyad, S., Swartz, M.A., De Palma, M., Iruela-Arispe, M.L. Perivascular macrophages limit permeability. ATVB 2016; 36: 2203-2212.
Inkeles, M.S., Teles, R.M., Pouldar, D., Andrade, P.R., Madigan, C.A., Lopez, D., Ambrose, M., Noursadeghi, M., Sarno E.N., Rea, T.H., Ochoa, M.T., Iruela-Arispe, M.L., Swindell, W.R., Ottenhoff, T.H., Geluk, A., Bloom, B.R., Pellegrini, M., Modlin, R.L. Cell-type deconvolution with immune pathways identifies gene networks of host defense and immunopathology in leprosy. JCI Insight 2016; 1(15): e88843.
Shirali, A.S., McDonald, A.I., Mack, J.J., Iruela-Arispe, M.L. Reproducible arterial denudation injury by infrarenal abdominal aortic clamping in a murine model. J Vis Exp 2016; (117): e54755.
Guihard, P.J., Yao, J., Blazquez-Medela, A.M., Iruela-Arispe, M.L., Bostrom, K.I., Yao, Y. Endothelial-Mesenchymal transition in vascular calcification of Ins2 Akita/+ Mice. . PLoS One 2016; 11(12): e54755.