Jau-Nian Chen, Ph.D.

Laboratory Address:
Room 490C, BSRB
Campus - 160606

Office Address:
Los Angeles, CA 90095

Work Address:
Los Angeles, CA 90095

Professor, Molecular, Cell, and Developmental Biology
Member, CTSI, Cell & Developmental Biology GPB Home Area, JCCC Cancer and Stem Cell Biology Program Area
Research Interests
The cardiovascular system is the first functioning organ during development. Abnormalities in the formation and/or function of the heart and vessels often lead to embryonic lethality or cause severe health issues in the adult. Our laboratory uses a multidisciplinary approach to understand the genetic, molecular and cellular basis of the cardiovascular system during normal development and in diseases. From classic and chemical genetic screens, we have identified mutations and compounds that affect proper patterning or function of the heart and vessels in the zebrafish. We are now using these mutants and compounds as points of entry to study questions about (1) the differential regulation of the arteries and veins during vessel formation and in the development of vascular diseases; (2) molecular hierarchies and cellular mechanisms guiding cardiac precursors to grow from the two-dimensional cardiac epithelium to a three-dimensional tubular structure; (3) molecular and physiological mechanisms by which rhythmic cardiac contraction is established and maintained and by which cardiac arrhythmia occurs; and (4) how the embryonic left-right asymmetry is established and how the heart develops with respect to the embryonic left-right axis.
Nguyen Catherine T, Langenbacher Adam, Hsieh Michael, Chen Jau-Nian The PAF1 complex component Leo1 is essential for cardiac and neural crest development in zebrafish. Developmental biology. 2010; 341(1): 167-75.
Wilson Christopher W, Nguyen Catherine T, Chen Miao-Hsueh, Yang Jehn-Hsiahn, Gacayan Rhodora, Huang Jie, Chen Jau-Nian, Chuang Pao-Tien Fused has evolved divergent roles in vertebrate Hedgehog signalling and motile ciliogenesis. Nature. 2009; 459(7243): 98-102.
Colantonio Jessica R, Vermot Julien, Wu David, Langenbacher Adam D, Fraser Scott, Chen Jau-Nian, Hill Kent L The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear. Nature. 2009; 457(7226): 205-9.
Langenbacher Adam, Chen Jau-Nian Calcium signaling: a common thread in vertebrate left-right axis development. Developmental dynamics : an official publication of the American Association of Anatomists. 2008; 237(12): 3491-6.
Xie Yi, Ottolia Michela, John Scott A, Chen Jau-Nian, Philipson Kenneth D Conformational changes of a Ca2+-binding domain of the Na+/Ca2+ exchanger monitored by FRET in transgenic zebrafish heart. American journal of physiology. Cell physiology. 2008; 295(2): C388-93.
Liu Jing, Fraser Sherri D, Faloon Patrick W, Rollins Evvi Lynn, Vom Berg Johannes, Starovic-Subota Olivera, Laliberte Angie L, Chen Jau-Nian, Serluca Fabrizio C, Childs Sarah J A betaPix Pak2a signaling pathway regulates cerebral vascular stability in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 2007; 104(35): 13990-5.
Lu Gang, Ren Shuxun, Korge Paavo, Choi Jayoung, Dong Yuan, Weiss James, Koehler Carla, Chen Jau-nian, Wang Yibin A novel mitochondrial matrix serine/threonine protein phosphatase regulates the mitochondria permeability transition pore and is essential for cellular survival and development. Genes & development. 2007; 21(7): 784-96.
Choi Jayoung, Dong Linda, Ahn Janice, Dao Diem, Hammerschmidt Matthias, Chen Jau-Nian FoxH1 negatively modulates flk1 gene expression and vascular formation in zebrafish. Developmental biology. 2007; 304(2): 735-44.
Cibrián-Uhalte Elena, Langenbacher Adam, Shu Xiaodong, Chen Jau-Nian, Abdelilah-Seyfried Salim Involvement of zebrafish Na+,K+ ATPase in myocardial cell junction maintenance. The Journal of cell biology. 2007; 176(2): 223-30.
Shu Xiaodong, Huang Jie, Dong Yuan, Choi Jayoung, Langenbacher Adam, Chen Jau-Nian Na,K-ATPase alpha2 and Ncx4a regulate zebrafish left-right patterning. Development (Cambridge, England). 2007; 134(10): 1921-30.
Kim HJ, Sumanas S, Palencia-Desai S, Dong Y, Chen JN and Lin S Genetic analysis of early endocrine pancreas formation in zebrafish. Molecular Endocrinology 2006; 20: 194-203.
Mably John D, Chuang Lesley P, Serluca Fabrizio C, Mohideen Manzoor-Ali P K, Chen Jau-Nian, Fishman Mark C santa and valentine pattern concentric growth of cardiac myocardium in the zebrafish. Development (Cambridge, England). 2006; 133(16): 3139-46.
Chen Jau-Nian, Cowan Douglas B, Mably John D Cardiogenesis and the regulation of cardiac-specific gene expression. Heart failure clinics. 2005; 1(2): 157-70.
Jin Suk-Won, Beis Dimitris, Mitchell Tracy, Chen Jau-Nian, Stainier Didier Y R Cellular and molecular analyses of vascular tube and lumen formation in zebrafish. Development (Cambridge, England). 2005; 132(23): 5199-209.
Beis Dimitris, Bartman Thomas, Jin Suk-Won, Scott Ian C, D'Amico Leonard A, Ober Elke A, Verkade Heather, Frantsve Julie, Field Holly A, Wehman Ann, Baier Herwig, Tallafuss Alexandra, Bally-Cuif Laure, Chen Jau-Nian, Stainier Didier Y R, Jungblut Benno Genetic and cellular analyses of zebrafish atrioventricular cushion and valve development. Development (Cambridge, England). 2005; 132(18): 4193-204.
Langenbacher Adam D, Dong Yuan, Shu Xiaodong, Choi Jayoung, Nicoll Debora A, Goldhaber Joshua I, Philipson Kenneth D, Chen Jau-Nian Mutation in sodium-calcium exchanger 1 (NCX1) causes cardiac fibrillation in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 2005; 102(49): 17699-704.
Huang Haigen, Zhang Bo, Hartenstein Parvana A, Chen Jau-nian, Lin Shuo NXT2 is required for embryonic heart development in zebrafish. BMC developmental biology. 2005; 5(2): 7.
Shu Xiaodong, Cheng Karen, Patel Neil, Chen Fuhua, Joseph Elaine, Tsai Huai-Jen, Chen Jau-Nian Na,K-ATPase is essential for embryonic heart development in the zebrafish. Development (Cambridge, England). 2003; 130(25): 6165-73.
Cross Laura M, Cook Marisa A, Lin Shuo, Chen Jau-Nian, Rubinstein Amy L Rapid analysis of angiogenesis drugs in a live fluorescent zebrafish assay. Arteriosclerosis, thrombosis, and vascular biology. 2003; 23(5): 911-2.
Mably John D, Mohideen Manzoor Ali P K, Burns C Geoffrey, Chen Jau-Nian, Fishman Mark C heart of glass regulates the concentric growth of the heart in zebrafish. Current biology : CB. 2003; 13(24): 2138-47.
Childs Sarah, Chen Jau-Nian, Garrity Deborah M, Fishman Mark C Patterning of angiogenesis in the zebrafish embryo. Development (Cambridge, England). 2002; 129(4): 973-82.
Peterson RT, Mably JD, Chen J-N and Fishman MC Convergence of distinct pathways to heart patterning reveals by the small molecule concentramide and the mutation heart-and-soul. Current Biology 2001; 11: 1481-1491.
Chan J, Mably JD, Serluca FC, Chen J-N, Goldstein NB, Thomas MC, Jennifer A. Cleary JA, Brennan C, Fishman MC and Roberts TM Formation of prechordal plate and notochord requires intact eph/ephrin signaling. Developmental Biology 2001; 234: 470-482.
Chen J-N, van Bebber F, Goldstein AM, Serluca FC, Jackson D, Childs S, Serbedzija G, Warren KS, Mably JD, Lindahl P, Mayer A, Haffter P, Fishman MC Genetic steps to organ laterality in zebrafish. Comparative and Functional Genomics 2001; 2: 60-68.
Chen J-N and Fishman MC Genetic dissection of zebrafish heart development. Fish, Fly, Worm and man 2000; 107-122.
Chen J-N and Fishman MC Genetics of Cardiac Development in Vertebrates. Trends in Genetics 2000; 16: 383-388.
Park M, Lewis C, Turbay D, Chung A, Chen JN, Evans S, Breitbart RE, Fishman MC, Izumo S, Bodmer R Differential rescue of visceral and cardiac defects in Drosophila by vertebrate tinman-related genes. Proceedings of the National Academy of Sciences of the United States of America. . 1998; 95(16): 9366-71.
Serbedzija GN, Chen JN, Fishman MC Regulation in the heart field of zebrafish. Development (Cambridge, England) . 1998; 125(6): 1095-101.
Chin AJ, Chen J-N, Weinberg ES. Bone morphogenetic protein-4 expression characterizes inductive boundaries in organs of developing zebrafish. Devel. Genes and Evolu 1997; 207: 107-114.
Chen J-N, Fishman MC Genetic dissection of heart development. Development of Cardiovascular System: Molecules to Organisms 1997; 7-17.
Chen JN, van Eeden FJ, Warren KS, Chin A, N??sslein-Volhard C, Haffter P, Fishman MC Left-right pattern of cardiac BMP4 may drive asymmetry of the heart in zebrafish. Development (Cambridge, England) . 1997; 124(21): 4373-82.
Chen JN, Haffter P, Odenthal J, Vogelsang E, Brand M, van Eeden FJ, Furutani-Seiki M, Granato M, Hammerschmidt M, Heisenberg CP, Jiang YJ, Kane DA, Kelsh RN, Mullins MC, N??sslein-Volhard C Mutations affecting the cardiovascular system and other internal organs in zebrafish. Development (Cambridge, England) . 1996; 123: 293-302.
Stainier DY, Fouquet B, Chen JN, Warren KS, Weinstein BM, Meiler SE, Mohideen MA, Neuhauss SC, Solnica-Krezel L, Schier AF, Zwartkruis F, Stemple DL, Malicki J, Driever W, Fishman MC Mutations affecting the formation and function of the cardiovascular system in the zebrafish embryo. Development (Cambridge, England) . 1996; 123: 285-92.
Chen J-N, Fishman MC. Zebrafish tinman homolog demarcates the heart field and initiates myocardial differentiation. Development 1996; 122: 3809-3816.
Murphy M, Chen JN, George DL Establishment and characterization of a human leptomeningeal cell line. Journal of neuroscience research. . 1991; 30(3): 475-83.