Leonard H. Rome, Ph.D.

Office Address:
615 Charles E. Young Drive South
BSRB 350A
Los Angeles, CA 90095

Affiliations
Affiliations
Professor, Biological Chemistry
Research Interests
We are interested in the biogenesis and function of subcellular organelles. We have been concentrating on novel cytosolic ribonucleoprotein particles (RNPs) called vaults. Vaults were discovered in our laboratory and found to exist in most eukaryotic cells. They have an intricate shape composed of multiple arches reminiscent of cathedral vaults, hence their name. Vault size (~74 x 42 x42 nm), shape and localization suggests that they may involved in nucleo-cytoplasmic transport. We are interested in elucidating the function of these unique structures and in manipulating their structure to give them new functions. We are using the baculovirus expression system to produce recombinant vaults in order to test the concept that vaults can have a broad nanosystems application as malleable nanocapsules. Toward this aim we are currently designing particles with encapsulated fluorescent probes and enzymatically active protein domains. In addition, a number of strategies are currently being considered to encapsulate chemically active small molecules into the vault particle. If successful, these vault nanocapsules can be bioengineered to allow their use in a wide variety of biological applications including drug delivery, biological sensors, enzyme delivery, controlled release, and nano-electrical machine (NEMS) applications.
Biography

Leonard H. Rome is a cell biologist and biochemist who has served on the UCLA School of Medicine faculty since he joined the Department of Biological Chemistry in 1979. He became a full professor in 1988 and wAs Senior Associate Dean for Research in the School of Medicine from 1997 - 2012. Dr. Rome earned his B.S. in Chemistry and M.S. and Ph.D. in Biological Chemistry at the University of Michigan, Ann Arbor. He was a postdoctoral fellow at the National Institutes of Health, where he worked on lysosome biogenesis. Dr. Rome has chaired the School of Medicine Faculty Executive Committee and is actively involved in graduate and medical education. He is a recipient of the School of Medicine Award for Excellence in Education. While Senior Associate Dean for Research, he organized a strategic plan for research in the School and spearheaded campus-wide efforts in genomics, proteomics, and computational biology. His laboratory research centers on a novel cellular organelle called a "vault" which was discovered in his laboratory. Dr. Rome is presently organizing a Nanoscience Interdisciplinnary Research Team, a collaboration of disciplines including cell biologists, engineers, chemists, and structural biologists who are engineering vaults so that they can be used in drug delivery.

Publications

A selected list of publications:

Upendra K. Kar, Minu K. Srivastava, ├ůsa Andersson, Felicita Baratelli, Min Huang, Valerie A. Kickhoefer, Steven M. Dubinett, Leonard H. Rome, Sherven Sharma   Novel CCL21-Vault Nanocapsule Intratumoral Delivery Inhibits Lung Cancer Growth PLoS One, 2011; 6(5): e18758.
Daniel C. Buehler, Daniel B. Toso, Valerie A. Kickhoefer, Z. Hong Zhou, Leonard H. Rome   Vaults Engineered for Hydrophobic Drug Delivery Small, 2011; Epub ahead of print: .
Xia Y, Ramgopal Y, Li H, Shang L, Srinivas P, Kickhoefer VA, Rome LH, Preiser PR, Boey F, Zhang H, Venkatraman SS   Immobilization of Recombinant Vault Nanoparticles on Solid Substrates ACS Nano, 2010; 4(3): 1417-24.
Yang J, Kickhoefer VA, Ng BC, Gopal A, Bentolila LA, John S, Tolbert SH, Rome LH   Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange ACS Nano, 2010; 4(12): 7229-40.
Goldsmith LE, Pupols M, Kickhoefer VA, Rome LH, Monbouquette HG   Utilization of a protein "shuttle" to load vault nanocapsules with gold probes and proteins ACS Nano, 2009; 3(10): 3175-83.
Lai CY, Wiethoff CM, Kickhoefer VA, Rome LH, Nemerow GR   Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer ACS Nano, 2009; 3(3): 691-9.
Kong, L. B., Siva, A. C., Kickhoefer, V. A., Rome, L. H., Stewart, P. L.   RNA location and modeling of a WD40 repeat domain within the vault , 2000; 6(6): 890-900.
Kar Upendra K, Jiang Janina, Champion Cheryl I, Salehi Sahar, Srivastava Minu, Sharma Sherven, Rabizadeh Shahrooz, Niazi Kayvan, Kickhoefer Valerie, Rome Leonard H, Kelly Kathleen A   Vault nanocapsules as adjuvants favor cell-mediated over antibody-mediated immune responses following immunization of mice PloS one, 2012; 7(7): e38553.
Yang Jian, Srinivasan Aswin, Sun Yang, Mrazek Jan, Shu Zhanyong, Kickhoefer Valerie A, Rome Leonard H   Vault nanoparticles engineered with the protein transduction domain, TAT48, enhances cellular uptake Integrative biology : quantitative biosciences from nano to macro, 2012; .
Han Muri, Kickhoefer Valerie A, Nemerow Glen R, Rome Leonard H   Targeted vault nanoparticles engineered with an endosomolytic peptide deliver biomolecules to the cytoplasm ACS nano, 2011; 5(8): 6128-37.
Buehler Daniel C, Toso Daniel B, Kickhoefer Valerie A, Zhou Z Hong, Rome Leonard H   Vaults engineered for hydrophobic drug delivery Small (Weinheim an der Bergstrasse, Germany), 2011; 7(10): 1432-9.
Yang Jian, Kickhoefer Valerie A, Ng Benny C, Gopal Ajaykumar, Bentolila Laurent A, John Scott, Tolbert Sarah H, Rome Leonard H   Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange ACS nano, 2010; 4(12): 7229-40.
Xia Yun, Ramgopal Yamini, Li Hai, Shang Lei, Srinivas Parisa, Kickhoefer Valerie A, Rome Leonard H, Preiser Peter R, Boey Freddy, Zhang Hua, Venkatraman Subbu S   Immobilization of recombinant vault nanoparticles on solid substrates ACS nano, 2010; 4(3): 1417-24.
Goldsmith Lisa E, Pupols Melody, Kickhoefer Valerie A, Rome Leonard H, Monbouquette Harold G   Utilization of a protein "shuttle" to load vault nanocapsules with gold probes and proteins ACS nano, 2009; 3(10): 3175-83.
Lai Cheng-Yu, Wiethoff Chris M, Kickhoefer Valerie A, Rome Leonard H, Nemerow Glen R   Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer ACS nano, 2009; 3(3): 691-9.
Kickhoefer, V. A., Han, M., Raval-Fernandes, S., Poderycki, M. J., Moniz, R. J., Vaccari, D., Silvestry, M., Stewart, P. L., Kelly, K. A., Rome, L. H.   Targeting vault nanoparticles to specific cell surface receptors ACS Nano, 2009; 3(1): 27-36.
Champion Cheryl I, Kickhoefer Valerie A, Liu Guangchao, Moniz Raymond J, Freed Amanda S, Bergmann Liisa L, Vaccari Dana, Raval-Fernandes Sujna, Chan Ann M, Rome Leonard H, Kelly Kathleen A   A vault nanoparticle vaccine induces protective mucosal immunity PloS one, 2009; 4(4): e5409.
Kickhoefer Valerie A, Han Muri, Raval-Fernandes Sujna, Poderycki Michael J, Moniz Raymond J, Vaccari Dana, Silvestry Mariena, Stewart Phoebe L, Kelly Kathleen A, Rome Leonard H   Targeting vault nanoparticles to specific cell surface receptors ACS nano, 2009; 3(1): 27-36.
Ng, B.C., Yu, M., Gopal, A., Rome, L.H., Monbouquette, H.G., Tolbert, S.H.   Encapsulation of semiconducting polymers in vault protein cages Nano Letters, 2008; 8(10): 3503-9.
Yu, M., Ng, B.C., Rome, L.H., Tolbert, S.H., Monbouquette, H.G.   Reversible pH lability of cross-linked vault nanocapsules Nano Letters, 2008; 8(10): 3510-5.
Esfandiary, R., Kickhoefer, V.A., Rome, L.H., Joshi, S.B., Middaugh, C.R.   Structural stability of vault particles Journal of Pharmaceutical Sciences, 2008; 98(4): 1376-86.
Xia, T., Rome, L.H., Nel, A.   Nanobiology: Particles slip cell security Nature Materials, 2008; 7(7): 519-20.
Anderson, D.H., Kickhoefer, V.A., Sievers, S.A., Rome, L.H., Eisenberg, D.   Draft crystal structure of the vault shell at 9-A resolution PLoS biology, 2007; 5(11): e318.
Kaddis, C.S., Lomeli, S.H., Yin, S., Berhane, B., Apostol, M.I., Kickhoefer, V.A., Rome, L.H., Loo, J.A.   Sizing large proteins and protein complexes by electrospray ionization mass spectrometry and ion mobility Journal of the American Society for Mass Spectrometry, 2007; 18(7): 1206-16.
Goldsmith, L.E., Yu, M., Rome, L.H., Monbouquette, H.G.   Vault nanocapsule dissociation into halves triggered at low pH Biochemistry, 2007; 46(10): 2865-75.
Poderycki, M.J., Kickhoefer, V.A., Kaddis, C.S., Raval-Fernandes, S., Johansson, E., Zink, J.I., Loo, J.A., Rome, L.H.   The vault exterior shell is a dynamic structure that allows incorporation of vault-associated proteins into its interior Biochemistry, 2006; 45(39): 12184-93.
Slesina, M., Inman, E.M., Moore, A.E., Goldhaber, J.I., Rome, L.H., Volknandt, W.   Movement of vault particles visualized by GFP-tagged major vault protein Cell and Tissue Research, 2006; 324(3): 403-10.
Raval-Fernandes, S., Kickhoefer, V.A., Kitchen, C., Rome, L.H.   Increased susceptibility of vault poly(ADP-ribose) polymerase-deficient mice to carcinogen-induced tumorigenesis Cancer Research, 2005; 65(19): 8846-52.
Slesina, M., Inman, E.M., Rome, L.H., Volknandt, W.   Nuclear localization of the major vault protein in U373 cells Cell and Tissue Research, 2005; 321(1): 97-104.
Kickhoefer, V.A., Garcia, Y., Mikyas, Y., Johansson, E., Zhou, J.C., Raval-Fernandes, S., Minoofar, P., Zink, J.I., Dunn, B., Stewart, P.L., Rome, L.H.   Engineering of vault nanocapsules with enzymatic and fluorescent properties Proceedings of the National Academy of Sciences of the United States of America, 2005; 102(12): 4348-52.
Poderycki, M.J., Rome, L.H., Harrington, L., Kickhoefer, V.A.   The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle Nucleic Acids Research, 2005; 33(3): 893-902.
Mikyas, Y., Makabi, M., Raval-Fernandes, S., Harrington, L., Kickhoefer, V.A., Rome, L.H., Stewart, P.L.   Cryoelectron microscopy imaging of recombinant and tissue derived vaults: localization of the MVP N termini and VPARP Journal of Molecular Biology, 2004; 344(1): 91-105.
Liu, Y., Snow, B.E., Kickhoefer, V.A., Erdmann, N., Zhou, W., Wakeham, A., Gomez, M., Rome, L.H., Harrington, L.   Vault poly(ADP-ribose) polymerase is associated with mammalian telomerase and is dispensable for telomerase function and vault structure in vivo Molecular and Cellular Biology, 2004; 24(12): 5314-23.
Emre, N., Raval-Fernandes, S., Kickhoefer, V.A., Rome, L.H.   Analysis of MVP and VPARP promoters indicates a role for chromatin remodeling in the regulation of MVP Biochimica et Biophysica Acta, 2004; 1678(1): 33-46.
Kickhoefer, V.A., Emre, N., Stephen, A.G., Poderycki, M.J., Rome, L.H.   Identification of conserved vault RNA expression elements and a non-expressed mouse vault RNA gene Gene, 2003; 309(2): 65-70.
Kickhoefer, V.A., Poderycki, M.J., Chan, E.K.L., Rome, L.H.   The La RNA-binding protein interacts with the vault RNA and is a vault-associated protein The Journal of Biological Chemistry, 2002; 277(43): 41282-6.
Hu, Y., Stephen, A.G., Cao, J., Tanzer, L.R., Slapak, C.A., Harrison, S.D., Devanarayan, V., Dantzig, A.H., Starling, J.J., Rome, L.H., Moore, R.E.   A very early induction of major vault protein accompanied by increased drug resistance in U-937 cells International journal of cancer, 2002; 97(2): 149-56.
Stephen, A. G., Raval-Fernandes, S., Huynh, T., Torres, M., Kickhoefer, V. A., Rome, L. H.   Assembly of vault-like particles in insect cells expressing only the major vault protein J Biol Chem, 2001; 276(26): 23217-20.
Siva, A. C., Raval-Fernandes, S., Stephen, A. G., LaFemina, M. J., Scheper, R. J., Kickhoefer, V. A., Rome, L. H.   Up-regulation of vaults may be necessary but not sufficient for multidrug resistance Int J Cancer, 2001; 92(2): 195-202.
Kickhoefer, V. A., Liu, Y., Kong, L. B., Snow, B. E., Stewart, P. L., Harrington, L., Rome, L. H.   The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle J Cell Biol, 2001; 152(1): 157-64.
Marbois, B. N., Faull, K. F., Fluharty, A. L., Raval-Fernandes, S., Rome, L. H.   Analysis of sulfatide from rat cerebellum and multiple sclerosis white matter by negative ion electrospray mass spectrometry Biochim Biophys Acta, 2000; 1484(1): 59-70.
Raval-Fernandes S, Rome L H   Role of axonal components during myelination Microscopy research and technique, 1998; 41(5): 379-92.
Schroeijers A B, Siva A C, Scheffer G L, de Jong M C, Bolick S C, Dukers D F, Slootstra J W, Meloen R H, Wiemer E, Kickhoefer V A, Rome L H, Scheper R J   The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines Cancer research, 2000; 60(4): 1104-10.
Kickhoefer V A, Rajavel K S, Scheffer G L, Dalton W S, Scheper R J, Rome L H   Vaults are up-regulated in multidrug-resistant cancer cell lines The Journal of biological chemistry, 1998; 273(15): 8971-4.
Kickhoefer V A, Siva A C, Kedersha N L, Inman E M, Ruland C, Streuli M, Rome L H   The 193-kD vault protein, VPARP, is a novel poly(ADP-ribose) polymerase The Journal of cell biology, 1999; 146(5): 917-28.
Kickhoefer V A, Stephen A G, Harrington L, Robinson M O, Rome L H   Vaults and telomerase share a common subunit, TEP1 The Journal of biological chemistry, 1999; 274(46): 32712-7.
Raval-Fernandes S, Sawant L A, Aebersold R H, Ducret A, Rome L H   Axonal proteins involved in myelination: characterization of a collagen-like protein Developmental neuroscience, 1997; 19(5): 421-9.
Raval-Fernandes S, Kickhoefer V A, Rome L H   Cloning of a cDNA encoding a sequence-specific single-stranded-DNA-binding protein from Rattus norvegicus Gene, 1999; 237(1): 201-7.
Kickhoefer V A, Vasu S K, Rome L H   Vaults are the answer, what is the question? Trends in cell biology, 1996; 6(5): 174-8.
Kong L B, Siva A C, Rome L H, Stewart P L   Structure of the vault, a ubiquitous celular component Structure (London, England : 1993), 1999; 7(4): 371-9.
Bame K J, Rome L H   Acetyl-CoA: alpha-glucosaminide N-acetyltransferase from rat liver Methods in enzymology, 1987; 138(13): 607-11.
Bame K J, Rome L H   Genetic evidence for transmembrane acetylation by lysosomes Science (New York, N.Y.), 1986; 233(4768): 1087-9.
Kedersha N L, Rome L H   Isolation and characterization of a novel ribonucleoprotein particle: large structures contain a single species of small RNA The Journal of cell biology, 1986; 103(3): 699-709.
Bame K J, Rome L H   Acetyl-coenzyme A:alpha-glucosaminide N-acetyltransferase. Evidence for an active site histidine residue The Journal of biological chemistry, 1986; 261(22): 10127-32.
Kedersha N L, Rome L H   Preparative agarose gel electrophoresis for the purification of small organelles and particles Analytical biochemistry, 1986; 156(1): 161-70.
Tietze F, Rome L H, Butler J D, Harper G S, Gahl W A   Impaired clearance of free cystine from lysosome-enriched granular fractions of I-cell-disease fibroblasts The Biochemical journal, 1986; 237(1): 9-15.
Kedersha N L, Hill D F, Kronquist K E, Rome L H   Subpopulations of liver coated vesicles resolved by preparative agarose gel electrophoresis The Journal of cell biology, 1986; 103(1): 287-97.
Rome L H, Hill D F   Lysosomal degradation of glycoproteins and glycosaminoglycans. Efflux and recycling of sulphate and N-acetylhexosamines The Biochemical journal, 1986; 235(3): 707-13.
Rome L H, Bullock P N, Chiappelli F, Cardwell M, Adinolfi A M, Swanson D   Synthesis of a myelin-like membrane by oligodendrocytes in culture Journal of neuroscience research, 1986; 15(1): 49-65.
Bame K J, Rome L H   Acetyl coenzyme A: alpha-glucosaminide N-acetyltransferase. Evidence for a transmembrane acetylation mechanism The Journal of biological chemistry, 1985; 260(20): 11293-9.
Hill D F, Bullock P N, Chiappelli F, Rome L H   Binding and internalization of lysosomal enzymes by primary cultures of rat glia Journal of neuroscience research, 1985; 14(1): 35-47.
Mehrabian M, Bame K J, Rome L H   Interaction of rat liver lysosomal membranes with actin The Journal of cell biology, 1984; 99(2): 680-5.
Berg R A, Schwartz M L, Rome L H, Crystal R G   Lysosomal function in the degradation of defective collagen in cultured lung fibroblasts Biochemistry, 1984; 23(10): 2134-8.
Campbell C H, Rome L H   Coated vesicles from rat liver and calf brain contain lysosomal enzymes bound to mannose 6-phosphate receptors The Journal of biological chemistry, 1983; 258(21): 13347-52.
Rome L H, Hill D F, Bame K J, Crain L R   Utilization of exogenously added acetyl coenzyme A by intact isolated lysosomes The Journal of biological chemistry, 1983; 258(5): 3006-11.
Campbell C H, Miller A L, Rome L H   Incorporation of mannose 6-phosphate receptors into liposomes. Receptor topography and binding of alpha-mannosidase The Biochemical journal, 1983; 214(2): 413-9.
Campbell C H, Fine R E, Squicciarini J, Rome L H   Coated vesicles from rat liver and calf brain contain cryptic mannose 6-phosphate receptors The Journal of biological chemistry, 1983; 258(4): 2628-33.
Steiner A W, Rome L H   Assay and purification of a solubilized membrane receptor that binds the lysosomal enzyme alpha-L-iduronidase Archives of biochemistry and biophysics, 1982; 214(2): 681-7.
Rome L H   alpha-L-iduronidase from human kidney Methods in enzymology, 1982; 83(2): 578-82.
Rome L H   Uptake and binding of alpha-L-iduronidase Methods in enzymology, 1982; 83(2): 582-7.
Rome L H, Crain L R   Degradation of mucopolysaccharide in intact isolated lysosomes The Journal of biological chemistry, 1981; 256(21): 10763-8.
Rome L H, Miller J   Butanedione treatment reduces receptor binding of a lysosomal enzyme to cells and membranes Biochemical and biophysical research communications, 1980; 92(3): 986-93.
Rome L H, Garvin A J, Allietta M M, Neufeld E F   Two species of lysosomal organelles in cultured human fibroblasts Cell, 1979; 17(1): 143-53.
Rome L H, Weissmann B, Neufeld E F   Direct demonstration of binding of a lysosomal enzyme, alpha-L-iduronidase, to receptors on cultured fibroblasts Proceedings of the National Academy of Sciences of the United States of America, 1979; 76(5): 2331-4.
Neufeld E F, Sando G N, Garvin A J, Rome L H   The transport of lysosomal enzymes Journal of supramolecular structure, 1977; 6(1): 95-101.
Rome L H, Garvin A J, Neufeld E F   Human kidney alpha-L-iduronidase: purification and characterization Archives of biochemistry and biophysics, 1978; 189(2): 344-53.
Lands W E, Cook H W, Rome L H   Prostaglandin biosynthesis: consequences of oxygenase mechanism upon in vitro assays of drug effectiveness Advances in prostaglandin and thromboxane research, 1976; 1(1): 7-17.
Rome L H, Lands W E, Roth G J, Majerus P W   Aspirin as a quantitative acetylating reagent for the fatty acid oxygenase that forms prostaglandins Prostaglandins, 1976; 11(1): 23-30.
Malek-Hedayat S, Rome L H   Cloning and sequence of the cDNA encoding the rat oligodendrocyte integrin beta 1 subunit Gene, 1995; 158(2): 287-90.
Rome L H   Multidrug resistance: locked in the vault? Nature medicine, 1995; 1(6): 527.
Kickhoefer V A, Rome L H   The sequence of a cDNA encoding the major vault protein from Rattus norvegicus Gene, 1994; 151(1-2): 257-60.
Vilalta A, Kickhoefer V A, Rome L H, Johnson D L   The rat vault RNA gene contains a unique RNA polymerase III promoter composed of both external and internal elements that function synergistically The Journal of biological chemistry, 1994; 269(47): 29752-9.
Hamilton S P, Rome L H   Stimulation of in vitro myelin synthesis by microglia Glia, 1994; 11(4): 326-35.
Notterpek L M, Rome L H   Functional evidence for the role of axolemma in CNS myelination Neuron, 1994; 13(2): 473-85.
Malek-Hedayat S, Rome L H   Expression of a beta 1-related integrin by oligodendroglia in primary culture: evidence for a functional role in myelination The Journal of cell biology, 1994; 124(6): 1039-46.
Notterpek L M, Rome L H   A protein involved in central nervous system myelination: localization in the extracellular matrix and induction in neuroblastoma cells Developmental neuroscience, 1994; 16(5-6): 267-78.
Matsumoto Nicholas M, Prabhakaran Panchami, Rome Leonard H, Maynard Heather D   Smart vaults: thermally-responsive protein nanocapsules ACS nano, 2013; 7(1): 867-74.
Notterpek L M, Bullock P N, Malek-Hedayat S, Fisher R, Rome L H   Myelination in cerebellar slice cultures: development of a system amenable to biochemical analysis Journal of neuroscience research, 1993; 36(6): 621-34.
Rome Leonard H, Kickhoefer Valerie A   Development of the Vault Particle as a Platform Technology ACS nano, 2012; 7(1): .
Grody W W, Kronquist K E, Lee E U, Edmond J, Rome L H   PCR-based cystic fibrosis (CF) carrier screening in a first-year medical student biochemistry laboratory American journal of human genetics, 1993; 53(6): 1352-5.
Chugani D C, Rome L H, Kedersha N L   Evidence that vault ribonucleoprotein particles localize to the nuclear pore complex Journal of cell science, 1993; 106 ( Pt 1)(6): 23-9.
Stephen A G, Raval-Fernandes S, Huynh T, Torres M, Kickhoefer V A, Rome L H   Assembly of vault-like particles in insect cells expressing only the major vault protein The Journal of biological chemistry, 2001; 276(26): 23217-20.
Vasu S K, Kedersha N L, Rome L H   cDNA cloning and disruption of the major vault protein alpha gene (mvpA) in Dictyostelium discoideum The Journal of biological chemistry, 1993; 268(21): 15356-60.
Siva A C, Raval-Fernandes S, Stephen A G, LaFemina M J, Scheper R J, Kickhoefer V A, Rome L H   Up-regulation of vaults may be necessary but not sufficient for multidrug resistance International journal of cancer. Journal international du cancer, 2001; 92(2): 195-202.
Kickhoefer V A, Searles R P, Kedersha N L, Garber M E, Johnson D L, Rome L H   Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III The Journal of biological chemistry, 1993; 268(11): 7868-73.
Kickhoefer V A, Liu Y, Kong L B, Snow B E, Stewart P L, Harrington L, Rome L H   The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle The Journal of cell biology, 2001; 152(1): 157-64.
Malek-Hedayat S, Rome L H   Expression of multiple integrins and extracellular matrix components by C6 glioma cells Journal of neuroscience research, 1992; 31(3): 470-8.
Kong L B, Siva A C, Kickhoefer V A, Rome L H, Stewart P L   RNA location and modeling of a WD40 repeat domain within the vault RNA (New York, N.Y.), 2000; 6(6): 890-900.
Kedersha N L, Heuser J E, Chugani D C, Rome L H   Vaults. III. Vault ribonucleoprotein particles open into flower-like structures with octagonal symmetry The Journal of cell biology, 1991; 112(2): 225-35.
Marbois B N, Faull K F, Fluharty A L, Raval-Fernandes S, Rome L H   Analysis of sulfatide from rat cerebellum and multiple sclerosis white matter by negative ion electrospray mass spectrometry Biochimica et biophysica acta, 2000; 1484(1): 59-70.
Chugani D C, Kedersha N L, Rome L H   Vault immunofluorescence in the brain: new insights regarding the origin of microglia The Journal of neuroscience : the official journal of the Society for Neuroscience, 1991; 11(1): 256-68.
Bullock P N, Rome L H   Glass micro-fibers: a model system for study of early events in myelination Journal of neuroscience research, 1990; 27(3): 383-93.
Vasu S K, Rome L H   Dictyostelium vaults: disruption of the major proteins reveals growth and morphological defects and uncovers a new associated protein The Journal of biological chemistry, 1995; 270(28): 16588-94.
Kedersha N L, Miquel M C, Bittner D, Rome L H   Vaults. II. Ribonucleoprotein structures are highly conserved among higher and lower eukaryotes The Journal of cell biology, 1990; 110(4): 895-901.
Rome L H, Cardwell M C, Bullock P N, Hamilton S P   Matrix interactions regulating myelinogenesis in cultured oligodendrocytes Advances in experimental medicine and biology, 1990; 265(4): 157-67.
Kedersha N L, Rome L H   Vaults: large cytoplasmic RNP's that associate with cytoskeletal elements Molecular biology reports, 1990; 14(2-3): 121-2.
Cardwell M C, Rome L H   RGD-containing peptides inhibit the synthesis of myelin-like membrane by cultured oligodendrocytes The Journal of cell biology, 1988; 107(4): 1551-9.
Cardwell M C, Rome L H   Evidence that an RGD-dependent receptor mediates the binding of oligodendrocytes to a novel ligand in a glial-derived matrix The Journal of cell biology, 1988; 107(4): 1541-9.
Westcott K R, Rome L H   Cation-independent mannose 6-phosphate receptor contains covalently bound fatty acid Journal of cellular biochemistry, 1988; 38(1): 23-33.
Lemansky P, Hasilik A, von Figura K, Helmy S, Fishman J, Fine R E, Kedersha N L, Rome L H   Lysosomal enzyme precursors in coated vesicles derived from the exocytic and endocytic pathways The Journal of cell biology, 1987; 104(6): 1743-8.
Westcott K R, Searles R P, Rome L H   Evidence for ligand- and pH-dependent conformational changes in liposome-associated mannose 6-phosphate receptor The Journal of biological chemistry, 1987; 262(13): 6101-7.
Mrazek Jan, Toso Daniel, Ryazantsev Sergey, Zhang Xing, Zhou Z Hong, Fernandez Beatriz Campo, Kickhoefer Valerie A, Rome Leonard H   Polyribosomes are molecular 3D nanoprinters that orchestrate the assembly of vault particles ACS nano, 2014; 8(11): 11552-9.
Buehler Daniel C, Marsden Matthew D, Shen Sean, Toso Daniel B, Wu Xiaomeng, Loo Joseph A, Zhou Z Hong, Kickhoefer Valerie A, Wender Paul A, Zack Jerome A, Rome Leonard H   Bioengineered vaults: self-assembling protein shell-lipophilic core nanoparticles for drug delivery ACS nano, 2014; 8(8): 7723-32.
Wang Meng, Abad Danny, Kickhoefer Valerie A, Rome Leonard H, Mahendra Shaily   Vault Nanoparticles Packaged with Enzymes as an Efficient Pollutant Biodegradation Technology ACS nano, 2015; 9(11): 10931-40.
Sharma Sherven, Zhu Li, Srivastava Minu K, Harris-White Marni, Huang Min, Lee Jay M, Rosen Fran, Lee Gina, Wang Gerald, Kickhoefer Valerie, Rome Leonard H, Baratelli Felicita, St John Maie, Reckamp Karen, Chul-Yang Seok, Hillinger Sven, Strieter Robert, Dubinett Steven   CCL21 Chemokine Therapy for Lung Cancer International trends in immunity, 2013; 1(1): 10-15.
Matsumoto Nicholas M, Buchman George W, Rome Leonard H, Maynard Heather D   Dual pH- and Temperature-Responsive Protein Nanoparticles European polymer journal, 2015; 69(11): 532-539.
Zhu Ye, Jiang Janina, Said-Sadier Najwane, Boxx Gale, Champion Cheryl, Tetlow Ashley, Kickhoefer Valerie A, Rome Leonard H, Ojcius David M, Kelly Kathleen A   Activation of the NLRP3 inflammasome by vault nanoparticles expressing a chlamydial epitope Vaccine, 2015; 33(2): 298-306.
Jiang Janina, Liu Guangchao, Kickhoefer Valerie A, Rome Leonard H, Li Lin-Xi, McSorley Stephen J, Kelly Kathleen A   A Protective Vaccine against Chlamydia Genital Infection Using Vault Nanoparticles without an Added Adjuvant Vaccines, 2017; 5(1): 872-881.
Ding Ke, Zhang Xing, Mrazek Jan, Kickhoefer Valerie A, Lai Mason, Ng Hwee L, Yang Otto O, Rome Leonard H, Zhou Z Hong   Solution Structures of Engineered Vault Particles Structure (London, England : 1993), 2018; 26(4): 619-626.e3.
Yu Kang, Yau Yin Hoe, Sinha Ameya, Tan Tabitha, Kickhoefer Valerie A, Rome Leonard H, Lee Hwankyu, Shochat Susana G, Lim Sierin   Modulation of the Vault Protein-Protein Interaction for Tuning of Molecular Release Scientific reports, 2017; 7(1): 14816.
Benner Nancy L, Zang Xiaoyu, Buehler Daniel C, Kickhoefer Valerie A, Rome Michael E, Rome Leonard H, Wender Paul A   Vault Nanoparticles: Chemical Modifications for Imaging and Enhanced Delivery ACS nano, 2017; 11(1): 872-881.