Jesse R Zamudio
Affiliations
Affiliations
Assistant Professor, Molecular, Cell, and Developmental Biology
Member, Cell & Developmental Biology GPB Home Area
Research Interests
The Zamudio laboratory is interested in how RNA and RNA-binding proteins establish the gene expression programs that determine cellular identity. Using computational and molecular approaches, they aim to map RNA-mediated interactions and define their roles in normal human development and disease. Mechanistic insight into functional RNAs has illuminated multiple regulatory layers needed for normal cellular function and will continue to offer new pathways to target for the treatment of human diseases.
Biography

Jesse Zamudio received his bachelor's degree from UCLA in Chemistry & Biochemistry. He received his Ph.D. from UCLA in the Department of Microbiology, Immunology and Molecular Genetics working in the laboratory of Dr. David Campbell and Dr. Nancy Sturm. For his thesis work, he characterized the first eukaryotic messenger RNA (mRNA) ribose cap methyltransferases. Although present in both the unique mRNA cap structure of human pathogenic Kinetoplastid protozoa and human mRNA, the proteins responsible for each had not been discovered. Based on evolutionary sequence conservation to known viral proteins, they were able to characterize a conserved eukaryotic protein family responsible for these modifications and determine their role in mRNA biogenesis and protein translation in kinetoplastids. These studies have aided the investigation of the human cap ribose methyltransferases implicated in early development.

Jesse pursued his postdoctoral research in the laboratory of Dr. Phil Sharp at the MIT Cancer Center. His research focused on quantitative approaches to characterize Regulatory RNAs in embryonic and adult stem cells. He aimed to confidently assay regulation by the mammalian RNA interference (RNAi) pathway and in doing so discovered new classes of mammalian small RNAs and principles determining regulatory activity. Current research in the lab is focused on characterizing functional RNAs in the control of cell state transitions during development and cancer progression.

He joined the MCDB faculty in January of 2016.

Publications

A selected list of publications:

Bosson Andrew D, Zamudio Jesse R, Sharp Phillip A   Endogenous miRNA and target concentrations determine susceptibility to potential ceRNA competition Molecular cell, 2014; 56(3): 347-59.
Dimitrova Nadya, Zamudio Jesse R, Jong Robyn M, Soukup Dylan, Resnick Rebecca, Sarma Kavitha, Ward Amanda J, Raj Arjun, Lee Jeannie T, Sharp Phillip A, Jacks Tyler   LincRNA-p21 activates p21 in cis to promote Polycomb target gene expression and to enforce the G1/S checkpoint Molecular cell, 2014; 54(5): 777-90.
Zamudio Jesse R, Kelly Timothy J, Sharp Phillip A   Argonaute-bound small RNAs from promoter-proximal RNA polymerase II Cell, 2014; 156(5): 920-34.
Werner Maria, Purta Elzbieta, Kaminska Katarzyna H, Cymerman Iwona A, Campbell David A, Mittra Bidyottam, Zamudio Jesse R, Sturm Nancy R, Jaworski Jacek, Bujnicki Janusz M   2'-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family Nucleic acids research, 2011; 39(11): 4756-68.
Flynn Ryan A, Almada Albert E, Zamudio Jesse R, Sharp Phillip A   Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome Proceedings of the National Academy of Sciences of the United States of America, 2011; 108(26): 10460-5.
Zamudio Jesse R, Mittra Bidyottam, Campbell David A, Sturm Nancy R   Hypermethylated cap 4 maximizes Trypanosoma brucei translation Molecular microbiology, 2009; 72(5): 1100-10.
Zamudio Jesse R, Mittra Bidyottam, Chattopadhyay Arnab, Wohlschlegel James A, Sturm Nancy R, Campbell David A   Trypanosoma brucei spliced leader RNA maturation by the cap 1 2'-O-ribose methyltransferase and SLA1 H/ACA snoRNA pseudouridine synthase complex Molecular and cellular biology, 2009; 29(5): 1202-11.
Mittra Bidyottam, Zamudio Jesse R, Bujnicki Janusz M, Stepinski Janusz, Darzynkiewicz Edward, Campbell David A, Sturm Nancy R   The TbMTr1 spliced leader RNA cap 1 2'-O-ribose methyltransferase from Trypanosoma brucei acts with substrate specificity The Journal of biological chemistry, 2008; 283(6): 3161-72.
Zamudio Jesse R, Mittra Bidyottam, Foldynová-Trantírková Silvie, Zeiner Gusti M, Lukes Julius, Bujnicki Janusz M, Sturm Nancy R, Campbell David A   The 2'-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei Molecular and cellular biology, 2007; 27(17): 6084-92.
Zamudio Jesse R, Mittra Bidyottam, Zeiner Gusti M, Feder Marcin, Bujnicki Janusz M, Sturm Nancy R, Campbell David A   Complete cap 4 formation is not required for viability in Trypanosoma brucei Eukaryotic cell, 2006; 5(6): 905-15.