|Director, Biochemistry, Biophysics & Structural Biology GPB Home Area|
|Associate Professor, Biological Chemistry|
|Member, Gene Regulation GPB Home Area, JCCC Gene Regulation Program Area|
Dr. Guo is a structural biologist and biochemist who joined the UCLA School of Medicine faculty in July 2004. He earned his B.S. in Biophysics at the Nankai University and M.S. in Physical Chemistry at the Peking University, both in China. Dr. Guo received his Ph.D. in Biochemistry and Biophysics at the University of Pennsylvania in 2000. In graduate school, he studied the Cre-loxP site-specific DNA recombination using X-ray crystallography. He was a postdoctoral fellow with Dr. Thomas Cech at the University of Colorado at Boulder, where he worked on the thermostability and crystal structure of large ribozymes.
Senturia, R., Laganowsky, A., Barr, I., Brooke D. Scheidemantle, B.D., and Guo, F. Dimerization and heme binding are conserved in amphibian and starfish homologues of the microRNA processing protein DGCR8. PLoS ONE. 2012; 7(7): e39688.
Gong, M., Chen, Y., Senturia, R., Ulgherait, M., Faller, M., and Guo, F. Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8. . Protein Sci. 2012; 21: 797-808.
Barr, I., Smith, A.T., Chen, Y., Senturia, R., Burstyn, J.N., and Guo, F. Ferric, not ferrous, heme activates RNA-binding protein DGCR8 for primary microRNA processing. Proc. Natl. Acad. Sci. USA. 2012; 109: 1919-1924.
Meer, E.J., Wang, D.O., Kim, S.M., Barr, I., Guo, F., and Martin, K.C. Identification of a cis-acting element that localizes mRNA to synapses. Proc. Natl. Acad. Sci. USA 2012; 109: 4639-4644.
Barr, I., Smith, A.T., Senturia, R., Chen, Y., Scheidemantle, B.D., Burstyn, J.N., and Guo, F. DiGeorge Critical Region 8 (DGCR8) is a double-cysteine-ligated heme protein. J. Biol. Chem. 2011; 286: 16716-25.
Faller, M., Toso, D., Matsunaga, M., Atanasov, I., Senturia, R., Chen, Y., Zhou, Z.H., and Guo, F. DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures. RNA. 2010; 16: 1570-1583.
Senturia, R., Faller, M., Yin, S., Loo, J.A., Cascio, D., Sawaya, M.R., Hwang, D., Clubb, R.T., and Guo, F. Structure of the dimerization domain of DiGeorge Critical Region 8. Protein Sci. 2010; 19: 1354-1365.
Miallau Linda, Faller Michael, Chiang Janet, Arbing Mark, Guo Feng, Cascio Duilio, Eisenberg David Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. J, Biol. Chem. 2009; 284(1): 276-83.
Wang, Q., Barr, I., Guo, F., Lee, C. Evidence of a novel RNA secondary structure in the coding region of HIV-1 pol gene. RNA. 2008; 14(12): 2478-88.
Faller Michael, Guo Feng MicroRNA biogenesis: there's more than one way to skin a cat. Biochimica et biophysica acta. 2008; 1779(11): 663-7.
Faller, M., Matsunaga, M., Yin, S., Loo, J., and Guo, F. Heme is involved in microRNA processing. Nature Structural & Molecular Biology 2007; 14(1): 23-29.
Guo, F., Gooding, A.R. and Cech, T.R. Comparison of crystal structure interactions and thermodynamics for stabilizing mutations in the Tetrahymena ribozyme. RNA 2006; 12: 387-395.
Guo, F Gooding, AR Cech, TR Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site. Molecular cell. . 2004; 16(3): 351-62.
Guo, F Cech, TR Evolution of Tetrahymena ribozyme mutants with increased structural stability. Nature structural biology. . 2002; 9(11): 855-61.
Guo, F Cech, TR In vivo selection of better self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron. RNA (New York, N.Y.) . 2002; 8(5): 647-58.
Guo, F Gopaul, DN Van Duyne, GD Asymmetric DNA bending in the Cre-loxP site-specific recombination synapse. Proceedings of the National Academy of Sciences of the United States of America. . 1999; 96(13): 7143-8.
Gopaul, DN Guo, F Van Duyne, GD Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination. The EMBO journal. . 1998; 17(14): 4175-87.
Guo, F., Gopaul, D.N., van Duyne, G.D. Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse. Nature. 1997; 389(6646): 40-6.
Weitz, S. H., Gong, M., Barr, I., Weiss, S., and Guo, F. Processing of microRNA primary transcripts requires heme in mammalian cells. Proc. Natl. Acad. Sci. USA. 2014; 111: 1861-1866.
Quick-Cleveland, J., Jacob, J.P., Weitz, S.H., Shoffner, G., Senturia, R., and Guo, F. The DGCR8 RNA-binding heme domain recognizes primary microRNAs by clamping the hairpin. Cell Rep. 2014; 7(6): 1994-2005.
Chin, R.M., Fu, X., Pai, M.Y., Vergnes, L., Hwang, H., Deng, G., Diep, S., Lomenick, B., Meli, V.S., Monsalve, G.C., Hu, E., Whelan, S.A., Wang, J.X., Jung, G., Solis, G.M., Fazlollahi, F., Kaweeteerawat, C., Quach, A., Nili, M., Krall, A.S., Godwin, H.A., Chang, H.R., Faull, K.F., Guo, F., Jiang, M., Trauger, S.A., Saghatelian, A., Braas, D., Christofk, H.R., Clarke, C.F., Teitell, M.A., Petrascheck, M., Reue, K., Jung, M.E., Frand, A.R., and Huang, J. The metabolite α-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR. Nature. 2014; 510: 397-410.
Barr, I. and Guo, F. Primary microRNA processing assay reconstituted using recombinant Drosha and DGCR8. Methods Mol. Biol. 2014; 1095: 73-86.