Feng Guo, Ph.D.

Laboratory Address:
611 Charles E. Young Dr. E
Los Angeles, CA 90095

Work Address:
Boyer 202

Affiliations
Affiliations
Director, Biochemistry, Biophysics & Structural Biology GPB Home Area
Associate Professor, Biological Chemistry
Member, Gene Regulation GPB Home Area, JCCC Gene Regulation Program Area
Research Interests
My research group is interested to understand how non-coding RNAs function by forming defined three-dimensional structures and by interacting with proteins. In particular, we are investigating how a class of small RNAs (called microRNA or miRNA) is processed in human. The projects will be investigated using X-ray crystallography, biochemistry and in vitro evolution methods. MiRNAs are involved in many important biological functions, and are emerged as a new class of oncogenes and tumor suppressors. They regulate the expression of many protein-coding genes by targeting their messenger RNAs (mRNAs) for degradation or translational repression. To become the short mature functional forms, the long primary transcripts (pri-miRNAs) need to be specifically recognized and cleaved by a series of cellular processing factors. The first step of this processing pathway involves two protein factors, Drosha (a ribonuclease III family member) and DGCR8 (an RNA binding protein). We are characterizing the RNA binding and cleavage properties of these proteins, in the hope to use the knowledge to improve the algorithms to predict new miRNA genes and to design gene knockdown technologies by mimicking pri-miRNA. We recently found surprisingly that DGCR8 is a heme-binding protein and heme is likely involved in the regulation of miRNA processing. Our findings have important implications to the biological functions of miRNAs and heme, and to the pathogenesis and therapies of diseases such as cancers and Alzheimer's disease.
Biography

Dr. Guo is a structural biologist and biochemist who joined the UCLA School of Medicine faculty in July 2004. He earned his B.S. in Biophysics at the Nankai University and M.S. in Physical Chemistry at the Peking University, both in China. Dr. Guo received his Ph.D. in Biochemistry and Biophysics at the University of Pennsylvania in 2000. In graduate school, he studied the Cre-loxP site-specific DNA recombination using X-ray crystallography. He was a postdoctoral fellow with Dr. Thomas Cech at the University of Colorado at Boulder, where he worked on the thermostability and crystal structure of large ribozymes.

Publications

A selected list of publications:

Senturia, R., Laganowsky, A., Barr, I., Brooke D. Scheidemantle, B.D., and Guo, F.   Dimerization and heme binding are conserved in amphibian and starfish homologues of the microRNA processing protein DGCR8 PLoS ONE, 2012; 7(7): e39688.
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Gong, M., Chen, Y., Senturia, R., Ulgherait, M., Faller, M., and Guo, F.   Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8. Protein Sci, 2012; 21: 797-808.
Barr, I., Smith, A.T., Chen, Y., Senturia, R., Burstyn, J.N., and Guo, F.   Ferric, not ferrous, heme activates RNA-binding protein DGCR8 for primary microRNA processing Proc. Natl. Acad. Sci. USA, 2012; 109: 1919-1924.
Meer, E.J., Wang, D.O., Kim, S.M., Barr, I., Guo, F., and Martin, K.C.   Identification of a cis-acting element that localizes mRNA to synapses, Proc. Natl. Acad. Sci. USA, 2012; 109: 4639-4644.
Barr, I., Smith, A.T., Senturia, R., Chen, Y., Scheidemantle, B.D., Burstyn, J.N., and Guo, F.   DiGeorge Critical Region 8 (DGCR8) is a double-cysteine-ligated heme protein J. Biol. Chem, 2011; 286: 16716-25.
Faller, M., Toso, D., Matsunaga, M., Atanasov, I., Senturia, R., Chen, Y., Zhou, Z.H., and Guo, F.   DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures RNA, 2010; 16: 1570-1583.
Senturia, R., Faller, M., Yin, S., Loo, J.A., Cascio, D., Sawaya, M.R., Hwang, D., Clubb, R.T., and Guo, F.   Structure of the dimerization domain of DiGeorge Critical Region 8 Protein Sci, 2010; 19: 1354-1365.
Miallau Linda, Faller Michael, Chiang Janet, Arbing Mark, Guo Feng, Cascio Duilio, Eisenberg David   Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis J, Biol. Chem, 2009; 284(1): 276-83.
Wang, Q., Barr, I., Guo, F., Lee, C.   Evidence of a novel RNA secondary structure in the coding region of HIV-1 pol gene RNA, 2008; 14(12): 2478-88.
Faller Michael, Guo Feng   MicroRNA biogenesis: there's more than one way to skin a cat Biochimica et biophysica acta, 2008; 1779(11): 663-7.
Faller, M., Matsunaga, M., Yin, S., Loo, J., and Guo, F.   Heme is involved in microRNA processing, Nature Structural & Molecular Biology, 2007; 14(1): 23-29.
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Guo, F., Gooding, A.R. and Cech, T.R.   Comparison of crystal structure interactions and thermodynamics for stabilizing mutations in the Tetrahymena ribozyme, RNA, 2006; 12: 387-395.
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Guo, F Gooding, AR Cech, TR   Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site Molecular cell. , 2004; 16(3): 351-62.
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Guo, F Cech, TR   Evolution of Tetrahymena ribozyme mutants with increased structural stability Nature structural biology. , 2002; 9(11): 855-61.
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Guo, F Cech, TR   In vivo selection of better self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron RNA (New York, N.Y.) , 2002; 8(5): 647-58.
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Guo, F Gopaul, DN Van Duyne, GD   Asymmetric DNA bending in the Cre-loxP site-specific recombination synapse Proceedings of the National Academy of Sciences of the United States of America. , 1999; 96(13): 7143-8.
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Gopaul, DN Guo, F Van Duyne, GD   Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination The EMBO journal. , 1998; 17(14): 4175-87.
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Guo, F., Gopaul, D.N., van Duyne, G.D.   Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse Nature, 1997; 389(6646): 40-6.
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Weitz, S. H., Gong, M., Barr, I., Weiss, S., and Guo, F.   Processing of microRNA primary transcripts requires heme in mammalian cells Proc. Natl. Acad. Sci. USA, 2014; 111: 1861-1866.
Quick-Cleveland, J., Jacob, J.P., Weitz, S.H., Shoffner, G., Senturia, R., and Guo, F.   The DGCR8 RNA-binding heme domain recognizes primary microRNAs by clamping the hairpin Cell Rep, 2014; 7(6): 1994-2005.
Chin, R.M., Fu, X., Pai, M.Y., Vergnes, L., Hwang, H., Deng, G., Diep, S., Lomenick, B., Meli, V.S., Monsalve, G.C., Hu, E., Whelan, S.A., Wang, J.X., Jung, G., Solis, G.M., Fazlollahi, F., Kaweeteerawat, C., Quach, A., Nili, M., Krall, A.S., Godwin, H.A., Chang, H.R., Faull, K.F., Guo, F., Jiang, M., Trauger, S.A., Saghatelian, A., Braas, D., Christofk, H.R., Clarke, C.F., Teitell, M.A., Petrascheck, M., Reue, K., Jung, M.E., Frand, A.R., and Huang, J.   The metabolite α-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR Nature, 2014; 510: 397-410.
Barr, I. and Guo, F.   Primary microRNA processing assay reconstituted using recombinant Drosha and DGCR8 Methods Mol. Biol, 2014; 1095: 73-86.