Jeffrey Abramson, Ph.D.

Laboratory Address:
CHS 53-254

Work Address:
CHS 53-260
Mailcode 775118

Affiliations
Affiliations
Associate Professor, Physiology
Member, Biochemistry, Biophysics & Structural Biology GPB Home Area, Molecular, Cellular & Integrative Physiology GPB Home Area, themes
Research Interests
At least 20% of the genomes sequenced thus far encode for membrane proteins. Many of these proteins are involved in fundamental biological processes and have been linked with numerous human diseases, thus they are often primary targets for therapeutic drugs. However, the number of crystal structures of membrane proteins is very limited considering their vast numbers as well as crucial biological and medical relevance. We are attempting to expedite structural determination of membrane proteins by utilizing robotics for crystallization as well as visualization in order to increase throughput of membrane protein structures. The laboratory is focused on several membrane transport proteins with the goal of developing a detailed understanding of the mechanisms involved in these transport processes by utilizing the structure in conjunction with a biochemical and biophysical techniques.
Biography:

Jeff Abramson has recently joined the faculty of UCLA in January of 2005. Although his primary training is in Biochemistry and Structural Biology he was recruited by the Department of Physiology. Dr. Abramson earned his B.S. in Biotechnology at California Polytechnic University Pomona and his Ph.D. in Biochemistry at Uppsala University, Sweden. He then went to Imperial College London for his postdoctoral training where he furthered his skills in membrane protein crystallography. Dr. Abramson is currently collaborating with a number of groups within the Department of Physiology where he is utilizing robotics for crystallization as well as visualization in order to increase throughput of membrane protein structures.

Publications

A selected list of publications:

Nicoll DA, Sawaya MR, Kwon S, Cascio D, Philipson KD, Abramson J.   The crystal structure of the primary Ca2+ sensor of the Na+/Ca2+ exchanger reveals a novel Ca2+ binding motif J Biol Chem , 2006; 281(31): 21577-81.
Abramson, J. Iwata, S. Kaback, H. R.   Lactose permease as a paradigm for membrane transport proteins (Review) Mol Membr Biol, 2004; 21(4): 227-36.
Abramson, J Kaback, HR Iwata, S   Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily Current opinion in structural biology. , 2004; 14(4): 413-9.
Iwata, M. Abramson, J. Byrne, B. Iwata, S.   Structure and function of quinone binding membrane proteins Adv Protein Chem, 2003; 63: 151-76.
Abramson, J Smirnova, I Kasho, V Verner, G Kaback, HR Iwata, S   Structure and mechanism of the lactose permease of Escherichia coli Science. , 2003; 301(5633): 610-5.
Abramson, J Smirnova, I Kasho, V Verner, G Iwata, S Kaback, HR   The lactose permease of Escherichia coli: overall structure, the sugar-binding site and the alternating access model for transport FEBS letters. , 2003; 555(1): 96-101.
Jormakka, M. Tornroth, S. Abramson, J. Byrne, B. Iwata, S.   Purification and crystallization of the respiratory complex formate dehydrogenase-N from Escherichia coli Acta Crystallogr D Biol Crystallogr, 2002; 58(Pt 1): 160-2.
Svensson-Ek, M. Abramson, J. Larsson, G. Tornroth, S. Brzezinski, P. Iwata, S.   The X-ray crystal structures of wild-type and EQ(I-286) mutant cytochrome c oxidases from Rhodobacter sphaeroides J Mol Biol, 2002; 321(2): 329-39.
Abramson, J Svensson-Ek, M Byrne, B Iwata, S   Structure of cytochrome c oxidase: a comparison of the bacterial and mitochondrial enzymes Biochimica et biophysica acta. , 2001; 1544(1-2): 1-9.
Sivars, U Abramson, J Iwata, S Tjerneld, F   Affinity partitioning of a poly(histidine)-tagged integral membrane protein, cytochrome bo3 ubiquinol oxidase, in a detergent--polymer aqueous two-phase system containing metal-chelating polymer Journal of chromatography. B, Biomedical sciences and applications. , 2000; 743(1-2): 307-16.
Byrne, B Abramson, J Jansson, M Holmgren, E Iwata, S   Fusion protein approach to improve the crystal quality of cytochrome bo(3) ubiquinol oxidase from Escherichia coli Biochimica et biophysica acta. , 2000; 1459(2-3): 449-55.
Abramson, J Larsson, G Byrne, B Puustinen, A Garcia-Horsman, A Iwata, S   Purification, crystallization and preliminary crystallographic studies of an integral membrane protein, cytochrome bo3 ubiquinol oxidase from Escherichia coli Acta crystallographica. Section D, Biological crystallography. , 2000; 56 ( Pt 8): 1076-8.
Abramson, J Riistama, S Larsson, G Jasaitis, A Svensson-Ek, M Laakkonen, L Puustinen, A Iwata, S Wikström, M   The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site Nature structural biology. , 2000; 7(10): 910-7.