|Associate Professor, Pediatric Immunology, Allergy and Rheumatology|
|Member, Biochemistry, Biophysics & Structural Biology GPB Home Area, California NanoSystems Institute, themes|
|Faculty, Immunity, Microbes & Molecular Pathogenesis GPB Home Area|
Manish J. Butte, MD PhD is an Associate Professor in the Department of Pediatrics at UCLA, and Division Chief of Immunology, Allergy, and Rheumatology. He studied Physics at Brown University where he earned his Sc.B. with honors in 1993, studying mathematical neural networks in Prof. Leon Cooper's group. During this time he developed software for Microsoft and Apple, and performed research at the National Institutes of Health. He earned his M.D. degree from the Brown University School of Medicine in 1996. He then studied protein crystallography under Prof. Robert Fletterick at UCSF and graduated with a Ph.D. in Biophysics in 2000. Returning to clinical training, he completed a Pediatrics residency at the Children's Hospital of Philadelphia in 2003 and a clinical fellowship in Allergy & Immunology at Boston Children's Hospital in 2006, where he specialized in the care of children with immunodeficiencies, autoimmunity, auto-inflammatory disorders, asthma, and allergies. During a joint post-doctoral fellowship at Harvard Medical School (under Prof. Arlene Sharpe) and in the Harvard Chemistry & Chemical Biology Dept. (under Prof. George Whitesides), he worked on T cell inhibitory pathways and development of microfabricated tools to capture and study immune cells.
He transitioned to Stanford University in 2009 to start his own lab in the Department of Pediatrics, with courtesy appointments in the Department of Materials Science and Engineering and Microbiolology and Immunology. His lab addressed fundamental, long-standing questions in immunology using innovative nanotechnological approaches to visualize and manipulate cells. In November 2016, he moved his lab to UCLA.
A major focus of his lab is in understanding T cells, the major coordinating cells of the immune response, with a specific focus on mechanobiology. The group studies how mechanical forces influence T cells, with projects spanning multiple scales from the molecular level (how receptors in T cells sense forces) to the cellular (why T cells vigorously push and pull upon other cells) to the tissue (why T cells are hyper-functional in soft tissues, like autoimmune inflamed tissue, and poorly functional in stiff tissues, like cancers). His lab has innovated tools and techniques in the use of biological atomic force microscopy (AFM) and 2-photon intravital microscopy to interrogate cells and tissues.
The second major focus of his lab is in improving the diagnosis and treatment of genetic immune diseases. His group has developed CyTOF (cytometry by time of flight mass spectrometry) as a diagnostic tool for immune diseases. His lab has developed capabilities to study novel disease-causing mutations across a number of diseases in children and adults.
Dr. Butte is clinically active, and is board certified in both Pediatrics and Allergy & Immunology. He cares for children and adults in an Immunology and Immunodeficiency Clinic, which is a Jeffrey Modell Foundation Diagnostic Center.
Majedi Fatemeh S, Hasani-Sadrabadi Mohammad Mahdi, Kidani Yoko, Thauland Timothy J, Moshaverinia Alireza, Butte Manish J, Bensinger Steven J, Bouchard Louis-S Cytokine Secreting Microparticles Engineer the Fate and the Effector Functions of T-Cells. Advanced materials (Deerfield Beach, Fla.). 2018; 293(2): 567-578.
Nagy Nadine, de la Zerda Adi, Kaber Gernot, Johnson Pamela Y, Hu Kenneth H, Kratochvil Michael J, Yadava Koshika, Zhao Wenting, Cui Yi, Navarro Guadalupe, Annes Justin P, Wight Thomas N, Heilshorn Sarah C, Bollyky Paul L, Butte Manish J Hyaluronan content governs tissue stiffness in pancreatic islet inflammation. The Journal of biological chemistry. 2018; 293(2): 567-578.
Nyberg Kendra D, Hu Kenneth H, Kleinman Sara H, Khismatullin Damir B, Butte Manish J, Rowat Amy C Quantitative Deformability Cytometry: Rapid, Calibrated Measurements of Cell Mechanical Properties. Biophysical journal. 2017; 113(7): 1574-1584.
Choi Jeff, Fernandez Rosemary, Maecker Holden T, Butte Manish J Systems approach to uncover signaling networks in primary immunodeficiency diseases. The Journal of allergy and clinical immunology. 2017; 140(3): 881-884.e8.
Kulkarni Subhash, Micci Maria-Adelaide, Leser Jenna, Shin Changsik, Tang Shiue-Cheng, Fu Ya-Yuan, Liu Liansheng, Li Qian, Saha Monalee, Li Cuiping, Enikolopov Grigori, Becker Laren, Rakhilin Nikolai, Anderson Michael, Shen Xiling, Dong Xinzhong, Butte Manish J, Song Hongjun, Southard-Smith E Michelle, Kapur Raj P, Bogunovic Milena, Pasricha Pankaj J Adult enteric nervous system in health is maintained by a dynamic balance between neuronal apoptosis and neurogenesis. Proceedings of the National Academy of Sciences of the United States of America. 2017; 114(18): E3709-E3718.
Thauland Timothy J, Hu Kenneth H, Bruce Marc A, Butte Manish J Cytoskeletal adaptivity regulates T cell receptor signaling. Science signaling. 2017; 10(469): E3709-E3718.
Balaji Swathi, Wang Xinyi, King Alice, Le Louis D, Bhattacharya Sukanta S, Moles Chad M, Butte Manish J, de Jesus Perez Vinicio A, Liechty Kenneth W, Wight Thomas N, Crombleholme Timothy M, Bollyky Paul L, Keswani Sundeep G Interleukin-10-mediated regenerative postnatal tissue repair is dependent on regulation of hyaluronan metabolism via fibroblast-specific STAT3 signaling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2017; 31(3): 868-881.
Mascharak Shamik, Benitez Patrick L, Proctor Amy C, Madl Christopher M, Hu Kenneth H, Dewi Ruby E, Butte Manish J, Heilshorn Sarah C YAP-dependent mechanotransduction is required for proliferation and migration on native-like substrate topography. Biomaterials. 2017; 115(3): 155-166.
Lee Yu Nee, Frugoni Francesco, Dobbs Kerry, Tirosh Irit, Du Likun, Ververs Francesca A, Ru Heng, Ott de Bruin Lisa, Adeli Mehdi, Bleesing Jacob H, Buchbinder David, Butte Manish J, Cancrini Caterina, Chen Karin, Choo Sharon, Elfeky Reem A, Finocchi Andrea, Fuleihan Ramsay L, Gennery Andrew R, El-Ghoneimy Dalia H, Henderson Lauren A, Al-Herz Waleed, Hossny Elham, Nelson Robert P, Pai Sung-Yun, Patel Niraj C, Reda Shereen M, Soler-Palacin Pere, Somech Raz, Palma Paolo, Wu Hao, Giliani Silvia, Walter Jolan E, Notarangelo Luigi D Characterization of T and B cell repertoire diversity in patients with RAG deficiency. Science immunology. 2016; 1(6): 155-166.
Walter Jolan E, Rosen Lindsey B, Csomos Krisztian, Rosenberg Jacob M, Mathew Divij, Keszei Marton, Ujhazi Boglarka, Chen Karin, Lee Yu Nee, Tirosh Irit, Dobbs Kerry, Al-Herz Waleed, Cowan Morton J, Puck Jennifer, Bleesing Jack J, Grimley Michael S, Malech Harry, De Ravin Suk See, Gennery Andrew R, Abraham Roshini S, Joshi Avni Y, Boyce Thomas G, Butte Manish J, Nadeau Kari C, Balboni Imelda, Sullivan Kathleen E, Akhter Javeed, Adeli Mehdi, El-Feky Reem A, El-Ghoneimy Dalia H, Dbaibo Ghassan, Wakim Rima, Azzari Chiara, Palma Paolo, Cancrini Caterina, Capuder Kelly, Condino-Neto Antonio, Costa-Carvalho Beatriz T, Oliveira Joao Bosco, Roifman Chaim, Buchbinder David, Kumanovics Attila, Franco Jose Luis, Niehues Tim, Schuetz Catharina, Kuijpers Taco, Yee Christina, Chou Janet, Masaad Michel J, Geha Raif, Uzel Gulbu, Gelman Rebecca, Holland Steven M, Recher Mike, Utz Paul J, Browne Sarah K, Notarangelo Luigi D Broad-spectrum antibodies against self-antigens and cytokines in RAG deficiency. The Journal of clinical investigation. 2016; 126(11): 4389.
Butte Manish J, Park K T, Lewis David B Treatment of CGD-associated Colitis with the IL-23 Blocker Ustekinumab. Journal of clinical immunology. 2016; 36(7): 619-20.
Gebe John A, Yadava Koshika, Ruppert Shannon M, Marshall Payton, Hill Paul, Falk Ben A, Sweere Johanna M, Han Hongwei, Kaber Gernot, Medina Carlos, Mikecz Katalin, Ziegler Steven F, Balaji Swathi, Keswani Sundeep G, de Jesus Perez Vinicio A, Butte Manish J, Nadeau Kari, Altemeier William A, Fanger Neil, Bollyky Paul L Modified High Molecular Weight Hyaluronan Promotes Allergen-Specific Immune Tolerance. American journal of respiratory cell and molecular biology. 2016; 36(7): .
Hu Kenneth H, Butte Manish J T cell activation requires force generation. The Journal of cell biology. 2016; 213(5): 535-42.
Han Li-Hsin, Conrad Bogdan, Chung Michael T, Deveza Lorenzo, Jiang Xinyi, Wang Andrew, Butte Manish J, Longaker Michael T, Wan Derrick, Yang Fan Winner of the Young Investigator Award of the Society for Biomaterials at the 10th World Biomaterials Congress, May 17-22, 2016, Montreal QC, Canada: Microribbon-based hydrogels accelerate stem cell-based bone regeneration in a mouse critical-size cranial defect model. Journal of biomedical materials research. Part A. 2016; 104(6): 1321-31.
Nam Sungmin, Hu Kenneth H, Butte Manish J, Chaudhuri Ovijit Strain-enhanced stress relaxation impacts nonlinear elasticity in collagen gels. Proceedings of the National Academy of Sciences of the United States of America. 2016; 113(20): 5492-7.
Kuipers Hedwich F, Rieck Mary, Gurevich Irina, Nagy Nadine, Butte Manish J, Negrin Robert S, Wight Thomas N, Steinman Lawrence, Bollyky Paul L Hyaluronan synthesis is necessary for autoreactive T-cell trafficking, activation, and Th1 polarization. Proceedings of the National Academy of Sciences of the United States of America. 2016; 113(5): 1339-44.
Rael Efren, Rakszawski Kevin, Koller Kristian, Bayerl Michael, Butte Manish, Zheng Hong Treatment with rituximab and brentuximab vedotin in a patient of common variable immune deficiency-associated classic Hodgkin lymphoma. Biomarker research. 2016; 4(5): 7.
Wang Andrew, Vijayraghavan Karthik, Solgaard Olav, Butte Manish J Fast Stiffness Mapping of Cells Using High-Bandwidth Atomic Force Microscopy. ACS nano. 2016; 10(1): 257-64.
Walter Jolan E, Rosen Lindsey B, Csomos Krisztian, Rosenberg Jacob M, Mathew Divij, Keszei Marton, Ujhazi Boglarka, Chen Karin, Lee Yu Nee, Tirosh Irit, Dobbs Kerry, Al-Herz Waleed, Cowan Morton J, Puck Jennifer, Bleesing Jack J, Grimley Michael S, Malech Harry, De Ravin Suk See, Gennery Andrew R, Abraham Roshini S, Joshi Avni Y, Boyce Thomas G, Butte Manish J, Nadeau Kari C, Balboni Imelda, Sullivan Kathleen E, Akhter Javeed, Adeli Mehdi, El-Feky Reem A, El-Ghoneimy Dalia H, Dbaibo Ghassan, Wakim Rima, Azzari Chiara, Palma Paolo, Cancrini Caterina, Capuder Kelly, Condino-Neto Antonio, Costa-Carvalho Beatriz T, Oliveira Joao Bosco, Roifman Chaim, Buchbinder David, Kumanovics Attila, Franco Jose Luis, Niehues Tim, Schuetz Catharina, Kuijpers Taco, Yee Christina, Chou Janet, Masaad Michel J, Geha Raif, Uzel Gulbu, Gelman Rebecca, Holland Steven M, Recher Mike, Utz Paul J, Browne Sarah K, Notarangelo Luigi D Broad-spectrum antibodies against self-antigens and cytokines in RAG deficiency. The Journal of clinical investigation. 2015; 125(11): 4135-48.
Wei Ke, Serpooshan Vahid, Hurtado Cecilia, Diez-CuÃ±ado Marta, Zhao Mingming, Maruyama Sonomi, Zhu Wenhong, Fajardo Giovanni, Noseda Michela, Nakamura Kazuto, Tian Xueying, Liu Qiaozhen, Wang Andrew, Matsuura Yuka, Bushway Paul, Cai Wenqing, Savchenko Alex, Mahmoudi Morteza, Schneider Michael D, van den Hoff Maurice J B, Butte Manish J, Yang Phillip C, Walsh Kenneth, Zhou Bin, Bernstein Daniel, Mercola Mark, Ruiz-Lozano Pilar Epicardial FSTL1 reconstitution regenerates the adult mammalian heart. Nature. 2015; 525(7570): 479-85.
Hadad Uzi, Thauland Timothy J, Martinez Olivia M, Butte Manish J, Porgador Angel, Krams Sheri M NKp46 Clusters at the Immune Synapse and Regulates NK Cell Polarization. Frontiers in immunology. 2015; 6(7570): 495.
Mathieu Anne-Laure, Verronese Estelle, Rice Gillian I, Fouyssac Fanny, Bertrand Yves, Picard Capucine, Chansel Marie, Walter Jolan E, Notarangelo Luigi D, Butte Manish J, Nadeau Kari Christine, Csomos Krisztian, Chen David J, Chen Karin, Delgado Ana, Rigal Chantal, Bardin Christine, Schuetz Catharina, Moshous Despina, Reumaux HÃ©loÃ¯se, Plenat FranÃ§ois, Phan Alice, Zabot Marie-ThÃ©rÃ¨se, Balme Brigitte, Viel SÃ©bastien, Bienvenu Jacques, Cochat Pierre, van der Burg Mirjam, Caux Christophe, Kemp E Helen, Rouvet Isabelle, Malcus Christophe, MÃ©ritet Jean-Francois, Lim Annick, Crow Yanick J, Fabien Nicole, MÃ©nÃ©trier-Caux Christine, De Villartay Jean-Pierre, Walzer Thierry, Belot Alexandre PRKDC mutations associated with immunodeficiency, granuloma, and autoimmune regulator-dependent autoimmunity. The Journal of allergy and clinical immunology. 2015; 135(6): 1578-88.e5.
Matthews Adam G W, Briggs Christine E, Yamanaka Keiichi, Small Trudy N, Mooster Jana L, Bonilla Francisco A, Oettinger Marjorie A, Butte Manish J Compound heterozygous mutation of Rag1 leading to Omenn syndrome. PloS one. 2015; 10(4): e0121489.
Kanada Masamitsu, Bachmann Michael H, Hardy Jonathan W, Frimannson Daniel Omar, Bronsart Laura, Wang Andrew, Sylvester Matthew D, Schmidt Tobi L, Kaspar Roger L, Butte Manish J, Matin A C, Contag Christopher H Differential fates of biomolecules delivered to target cells via extracellular vesicles. Proceedings of the National Academy of Sciences of the United States of America. 2015; 112(12): E1433-42.
Thauland Timothy J, Butte Manish J Taking T cell priming down a Notch: signaling through Notch receptors enhances T cell sensitivity to antigen. Immunity. 2015; 42(1): 6-8.
Wang Andrew, Butte Manish J Customized atomic force microscopy probe by focused-ion-beam-assisted tip transfer. Applied physics letters. 2014; 105(5): 053101.
Burridge Paul W, Metzler Scott A, Nakayama Karina H, Abilez Oscar J, Simmons Chelsey S, Bruce Marc A, Matsuura Yuka, Kim Paul, Wu Joseph C, Butte Manish, Huang Ngan F, Yang Phillip C Multi-cellular interactions sustain long-term contractility of human pluripotent stem cell-derived cardiomyocytes. American journal of translational research. 2014; 6(6): 724-35.
Hu Kenneth K, Bruce Marc A, Butte Manish J Spatiotemporally and mechanically controlled triggering of mast cells using atomic force microscopy. Immunologic research. 2014; 58(2-3): 211-7.
Serpooshan Vahid, Zhao Mingming, Metzler Scott A, Wei Ke, Shah Parisha B, Wang Andrew, Mahmoudi Morteza, Malkovskiy Andrey V, Rajadas Jayakumar, Butte Manish J, Bernstein Daniel, Ruiz-Lozano Pilar The effect of bioengineered acellular collagen patch on cardiac remodeling and ventricular function post myocardial infarction. Biomaterials. 2013; 34(36): 9048-55.
Serpooshan Vahid, Zhao Mingming, Metzler Scott A, Wei Ke, Shah Parisha B, Wang Andrew, Mahmoudi Morteza, Malkovskiy Andrey V, Rajadas Jayakumar, Butte Manish J, Bernstein Daniel, Ruiz-Lozano Pilar Use of bio-mimetic three-dimensional technology in therapeutics for heart disease. Bioengineered. 2013; 5(3): 193-7.
Sun Wenchao, Araci Zeynep, Inayathullah Mohammed, Manickam Sathish, Zhang Xuexiang, Bruce Marc A, Marinkovich M Peter, Lane Alfred T, Milla Carlos, Rajadas Jayakumar, Butte Manish J Polyvinylpyrrolidone microneedles enable delivery of intact proteins for diagnostic and therapeutic applications. Acta biomaterialia. 2013; 9(8): 7767-74.
Bruce Marc A, Butte Manish J Real-time GPU-based 3D Deconvolution. Optics express. 2013; 21(4): 4766-73.
Ge Xiaohu, Wang I-Ning E, Toma Ildiko, Sebastiano Vittorio, Liu Jianwei, Butte Manish J, Reijo Pera Renee A, Yang Phillip C Human amniotic mesenchymal stem cell-derived induced pluripotent stem cells may generate a universal source of cardiac cells. Stem cells and development. 2012; 21(15): 2798-808.
Liu Jianwei, Butte Manish J Single molecule labeling of an atomic force microscope cantilever tip. Applied physics letters. 2012; 101(16): 163705.
Wang I-Ning E, Wang Xiang, Ge Xiaohu, Anderson Joshua, Ho Michael, Ashley Euan, Liu Jianwei, Butte Manish J, Yazawa Masayuki, Dolmetsch Ricardo E, Quertermous Thomas, Yang Phillip C Apelin enhances directed cardiac differentiation of mouse and human embryonic stem cells. PloS one. 2012; 7(6): e38328.
Liu Jianwei, Sun Ning, Bruce Marc A, Wu Joseph C, Butte Manish J Atomic force mechanobiology of pluripotent stem cell-derived cardiomyocytes. PloS one. 2012; 7(5): e37559.
Butte Manish J, Lee Sun Jung, Jesneck Jonathan, Keir Mary E, Haining W Nicholas, Sharpe Arlene H CD28 costimulation regulates genome-wide effects on alternative splicing. PloS one. 2012; 7(6): e40032.
Sun Ning, Yazawa Masayuki, Liu Jianwei, Han Leng, Sanchez-Freire Veronica, Abilez Oscar J, Navarrete Enrique G, Hu Shijun, Wang Li, Lee Andrew, Pavlovic Aleksandra, Lin Shin, Chen Rui, Hajjar Roger J, Snyder Michael P, Dolmetsch Ricardo E, Butte Manish J, Ashley Euan A, Longaker Michael T, Robbins Robert C, Wu Joseph C Patient-specific induced pluripotent stem cells as a model for familial dilated cardiomyopathy. Science translational medicine. 2012; 4(130): 130ra47.
Garcia Daniel, Ghansah Isaac, Leblanc John, Butte Manish J Counting cells with a low-cost integrated microfluidics-waveguide sensor. Biomicrofluidics. 2012; 6(1): 14115-141154.
Mack Eric T, Snyder Phillip W, Perez-Castillejos Raquel, BilgiÃ§er BaÅar, Moustakas Demetri T, Butte Manish J, Whitesides George M Dependence of avidity on linker length for a bivalent ligand-bivalent receptor model system. Journal of the American Chemical Society. 2012; 134(1): 333-45.
Leblanc John, Mueller Andrew J, Prinz Adrian, Butte Manish J Optical planar waveguide for cell counting. Applied physics letters. 2012; 100(4): 43701-437015.
Reches Meital, Mirica Katherine A, Dasgupta Rohit, Dickey Michael D, Butte Manish J, Whitesides George M Thread as a matrix for biomedical assays. ACS applied materials & interfaces. 2010; 2(6): 1722-8.
Butte Manish J, PeÃ±a-Cruz Victor, Kim Mi-Jung, Freeman Gordon J, Sharpe Arlene H Interaction of human PD-L1 and B7-1. Molecular immunology. 2008; 45(13): 3567-72.
McGuigan Alison P, Bruzewicz Derek A, Glavan Ana, Butte Manish J, Butte Manish, Whitesides George M Cell encapsulation in sub-mm sized gel modules using replica molding. PloS one. 2008; 3(5): e2258.
Keir Mary E, Butte Manish J, Freeman Gordon J, Sharpe Arlene H PD-1 and its ligands in tolerance and immunity. Annual review of immunology. 2008; 26(1): 677-704.
Kobayashi Norimoto, Karisola Piia, PeÃ±a-Cruz Victor, Dorfman David M, Jinushi Masahisa, Umetsu Sarah E, Butte Manish J, Nagumo Haruo, Chernova Irene, Zhu Baogong, Sharpe Arlene H, Ito Susumu, Dranoff Glenn, Kaplan Gerardo G, Casasnovas Jose M, Umetsu Dale T, Dekruyff Rosemarie H, Freeman Gordon J TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells. Immunity. 2007; 27(6): 927-40.
Butte Manish J, Haines Charles, Bonilla Francisco A, Puck Jennifer IL-7 receptor deficient SCID with a unique intronic mutation and post-transplant autoimmunity due to chronic GVHD. Clinical immunology (Orlando, Fla.). 2007; 125(2): 159-64.
Grabie Nir, Gotsman Israel, DaCosta Rosa, Pang Hong, Stavrakis George, Butte Manish J, Keir Mary E, Freeman Gordon J, Sharpe Arlene H, Lichtman Andrew H Endothelial programmed death-1 ligand 1 (PD-L1) regulates CD8+ T-cell mediated injury in the heart. Circulation. 2007; 116(18): 2062-71.
Martinez Andres W, Phillips Scott T, Butte Manish J, Whitesides George M Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angewandte Chemie (International ed. in English). 2007; 46(8): 1318-20.
Butte Manish J, Keir Mary E, Phamduy Theresa B, Sharpe Arlene H, Freeman Gordon J Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses. Immunity. 2007; 27(1): 111-22.
Read D, Butte M J, Dernburg A F, Frasch M, Kornberg T B Functional studies of the BTB domain in the Drosophila GAGA and Mod(mdg4) proteins. Nucleic acids research. 2000; 28(20): 3864-70.
Butte Manish J, Dodson Brenda, Dioun Anahita Pentobarbital desensitization in a 3-month-old child. Allergy and asthma proceedings. 2000; 25(4): 225-7.
Butte M J, Nguyen B X, Hutchison T J, Wiggins J W, Ziegler J W Pediatric myocardial infarction after racemic epinephrine administration. Pediatrics. 1999; 104(1): e9.
Butte M J, Hwang P K, Mobley W C, Fletterick R J Crystal structure of neurotrophin-3 homodimer shows distinct regions are used to bind its receptors. Biochemistry. 1998; 37(48): 16846-52.
Bertram R, Butte M J, Kiemel T, Sherman A Topological and phenomenological classification of bursting oscillations. Bulletin of mathematical biology. 1995; 57(3): 413-39.