Ambre M Bertholet, Ph.D.

Laboratory Address:
10833 Le Conte Avenue
10833 Le Conte Avenue, Los Angeles
Los Angeles, CA 90095

Office Address:
10833 Le Conte Avenue, Los Angeles
Los Angeles, CA 90095

Affiliations
Affiliations
Assistant Professor, Physiology
Member, Biochemistry, Biophysics & Structural Biology GPB Home Area
Research Interests

Mitochondria control cell metabolism by converting nutrients into an electrochemical gradient of protons (H+) across the inner mitochondrial membrane (IMM) to generate ATP, the currency of the cell, and heat (a process called mitochondrial thermogenesis). This energy conversion depends on H+ leaks across the IMM. A fine balance of H+ distribution between the two energy production forms, ATP and heat, defines the metabolic homeostasis of the cell. How mitochondria distribute energy between ATP and heat is certainly the most fundamental question in the field of bioenergetics, although it is still largely unknown. Our lab will explore the mechanisms of bioenergetics using the novel mitochondrial patch-clamp assay combined with modern cellular and molecular techniques with the goal of providing a fundamental understanding of how energy production and basal metabolism control physiology.

Biography:

Dr. Bertholet was trained in Toulouse, France at the Université Paul Sabatier. She earned her B.S. in Cell Biology and Physiology, M.S. in Gene Cells and Development, and her Ph.D. in Biology, Health and Biotechnologies. Subsequently, she did her postdoctoral training with Dr. Yuiry Kirichok at UC San Francisco and is currently an Assistant Research Physiologist.

Publications

A selected list of publications:

Bertholet Ambre M, Kirichok Yuriy   Patch-Clamp Analysis of the Mitochondrial H Frontiers in physiology, 2020; 11: 326.
Bertholet Ambre M   The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria Journal of visualized experiments : JoVE, 2021; (171): .
Allu Prasanna K R, Paulo Esther, Bertholet Ambre M, Situ Gavin, Lee Seung-Hwan, Wu Yixuan, Gleason Catherine E, Saha Bidisha, Chawla Ajay, Wang Biao, Pearce David   Role of mTORC2 in biphasic regulation of brown fat metabolism in response to mild and severe cold The Journal of biological chemistry, 2021; (171): 100632.
Bertholet Ambre M, Chouchani Edward T, Kazak Lawrence, Angelin Alessia, Fedorenko Andriy, Long Jonathan Z, Vidoni Sara, Garrity Ryan, Cho Joonseok, Terada Naohiro, Wallace Douglas C, Spiegelman Bruce M, Kirichok Yuriy   H Nature, 2019; 571(7766): 515-520.
Bertholet Ambre M, Kirichok Yuriy   The Mechanism FA-Dependent H Handbook of experimental pharmacology, 2019; 251(7766): 143-159.
Odegaard Justin I, Lee Min-Woo, Sogawa Yoshitaka, Bertholet Ambre M, Locksley Richard M, Weinberg David E, Kirichok Yuriy, Deo Rahul C, Chawla Ajay   Perinatal Licensing of Thermogenesis by IL-33 and ST2 Cell, 2017; 171(7): 1707.
Bertholet Ambre M, Kazak Lawrence, Chouchani Edward T, Bogaczyńska Marta G, Paranjpe Ishan, Wainwright Gabrielle L, Bétourné Alexandre, Kajimura Shingo, Spiegelman Bruce M, Kirichok Yuriy   Mitochondrial Patch Clamp of Beige Adipocytes Reveals UCP1-Positive and UCP1-Negative Cells Both Exhibiting Futile Creatine Cycling Cell metabolism, 2017; 25(4): 811-822.e4.
Bertholet Ambre M, Kirichok Yuriy   UCP1: A transporter for H Biochimie, 2017; 134(4): 28-34.
Odegaard Justin I, Lee Min-Woo, Sogawa Yoshitaka, Bertholet Ambre M, Locksley Richard M, Weinberg David E, Kirichok Yuriy, Deo Rahul C, Chawla Ajay   Perinatal Licensing of Thermogenesis by IL-33 and ST2 Cell, 2016; 166(4): 841-854.
Millet Aurélie M C, Bertholet Ambre M, Daloyau Marlène, Reynier Pascal, Galinier Anne, Devin Anne, Wissinguer Bernd, Belenguer Pascale, Davezac Noélie   Loss of functional OPA1 unbalances redox state: implications in dominant optic atrophy pathogenesis Annals of clinical and translational neurology, 2016; 3(6): 408-21.
Bertholet A M, Delerue T, Millet A M, Moulis M F, David C, Daloyau M, Arnauné-Pelloquin L, Davezac N, Mils V, Miquel M C, Rojo M, Belenguer P   Mitochondrial fusion/fission dynamics in neurodegeneration and neuronal plasticity Neurobiology of disease, 2016; 90: 3-19.
Bertholet Ambre M, Millet Aurélie M E, Guillermin Oriane, Daloyau Marlène, Davezac Noélie, Miquel Marie-Christine, Belenguer Pascale   OPA1 loss of function affects in vitro neuronal maturation Brain : a journal of neurology, 2013; 136(Pt 5): 1518-33.
Landes Thomas, Leroy Ingrid, Bertholet Ambre, Diot Alan, Khosrobakhsh Farnoosh, Daloyau Marlène, Davezac Noélie, Miquel Marie-Christine, Courilleau Delphine, Guillou Emmanuelle, Olichon Aurélien, Lenaers Guy, Arnauné-Pelloquin Laetitia, Emorine Laurent J, Belenguer Pascale   OPA1 (dys)functions Seminars in cell & developmental biology, 2010; 21(6): 593-8.
Betourne Alexandre, Bertholet Ambre M, Labroue Elodie, Halley Hélène, Sun Hong Shuo, Lorsignol Anne, Feng Zhong-Ping, French Robert J, Penicaud Luc, Lassalle Jean-Michel, Frances Bernard   Involvement of hippocampal CA3 K(ATP) channels in contextual memory Neuropharmacology, 2009; 56(3): 615-25.